Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers

Tibo, Luiz Henrique Soares; Bertól, Jéssica Wildgrube; Bernedo-Navarro, Robert Alvin; Yano, Tomomasa

doi:10.1016/j.bjid.2016.01.003

Cited by 3 publications

(1 citation statement)

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“…Vascular injury is one of the basic pathological changes in patients with sepsis, including destruction of vascular endothelial structure, abnormal vasoconstriction, and dysfunction of vascular endothelial cells, and it plays important roles in tissue and organ injuries induced by mild and severe sepsis [ 14 ]. Studies show that substances released to the blood by pathogens such as endotoxins can directly kill vascular endothelial cells, cause destruction of the vascular endothelial barrier, and lead to disorder of secretory function of endothelial cells, aggravating inflammation, and causing ischemic injury in tissues and organs [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Cells Activate Peripheral Natural Killer T Cells and Participate in Regulation of Downstream Immune Cascades in Patients with Sepsis

et al. 2018

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BackgroundThis study investigated the effect of supernatant of endothelial cells stimulated by peripheral blood serum from sepsis patients on phenotype and function of peripheral NKT cells.Material/MethodsTwenty-one patients with sepsis and 21 healthy subjects were included. Peripheral blood (5 ml) was collected from all patients and healthy subjects. To isolate peripheral blood mononuclear cells (PBMCs), Ficoll lymphocyte separation solution was used. Flow cytometry was carried out to determine NKT cell ratio, activity, and cytokine secretion. Human umbilical vein endothelial cells were cultured with serum from sepsis patients for 48 h before changing to fresh medium, and supernatant was collected. The supernatant was used to co-culture PBMCs before analyzing NKT activity and cytokines.ResultsThe ratios of CD3-CD56+NK cells and CD3+CD56+NKT cells were increased in peripheral blood from sepsis patients. Surface receptors p30, G2D, and p44 of CD3+CD56+NKT cells were elevated, while inhibitory receptors NKG2A and 158b were decreased. CD4+ NKT cells in peripheral blood from sepsis patients were enhanced. GranB, IFN-γ, IL-4, and IL-17 in NKT cells from sepsis patients were up-regulated. After co-culture with vascular endothelial cells treated with sepsis serum, expression of p30 and G2D in NKT cells was upregulated, and number of TCRVα24-positive cells was increased. In addition, ratio of CD4+NKT cells was increased, and intracellular expression of IL-4 and IFN-γ was elevated.ConclusionsThe study demonstrates that the level of NKT cells in peripheral blood from sepsis patients is increased, and their activity is enhanced. In addition, vascular endothelial cells from sepsis patients can regulate the activity of NKT cells.

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Section: Introductionmentioning

confidence: 99%

Vascular Endothelial Cells Activate Peripheral Natural Killer T Cells and Participate in Regulation of Downstream Immune Cascades in Patients with Sepsis

et al. 2018

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Antibacterial, proangiogenic, and osteopromotive nanoglass paste coordinates regenerative process following bacterial infection in hard tissue

et al. 2021

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Etiological structure of pig colibacillosis on the territory of the Russian Federation

Yakimova

Matyash

Belyaeva

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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In the course of the study, the results were obtained proving that on the territory of pig breeding enterprises in some regions of the Russian Federation, at least 29 E. Coli serogroups are circulating, both intestinal (enterotoxigenic - ETEC, enteropathogenic - EPEC, enterohemorragic - EHEC, enteroaggregative - EAEC, diffusely adherent - DAEC), and extraintestinal (uropathogenic - UPEC, neonatal meningitis - NMEC, avian pathogenic - APEC). The O2 serogroup has the largest share in this structure - 10.13%, then comes O4 – 9.13%, O20 – 6.98%, O41 – 6.95%, O35 – 5.74%, O55 – 5.62%, O33 – 5.38%, O26 – 5.02%, O119 – 4.14%, O111 – 3.93%, O18 – 3.23%, O103 – 2.99%, O8 – 2.96%, O1 and O126 - 2.93% each, O127 – 2.54%, O141 – 2.39%, O15 – 2.27%, O78 – 2.21%, O117 – 2.12%, O139 – 1.06%, O101 – 2.00%, O115 – 1.36%, O86 – 1.33%, O138 – 1.12%, O142 and O9 - 0.85 %, O149 – 0.67% and O3 – 0.18% each. Most of the E. Coli isolates were not serotyped due to the limited capabilities of the diagnostic kit. Most of the identified Escherichia serogroups (28 out of 29) were isolated from adult sows on a commercial reproducer, which suggests that this physiological group of animals is the main source of E. coli. At the same time, it was recorded that in addition to the vertical path of transmission of the pathogen (from sows to young animals), the horizontal path of transmission (for example, from people, with food, with water, with rodents, etc.) has an important role in the spread of colibacillosis. The results obtained can be useful for practicing veterinary specialists when planning therapeutic measures for pig colibacillosis.

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Cytotoxic factor secreted by Escherichia coli associated with sepsis facilitates transcytosis through human umbilical vein endothelial cell monolayers

Cited by 3 publications

References 20 publications

Vascular Endothelial Cells Activate Peripheral Natural Killer T Cells and Participate in Regulation of Downstream Immune Cascades in Patients with Sepsis

Vascular Endothelial Cells Activate Peripheral Natural Killer T Cells and Participate in Regulation of Downstream Immune Cascades in Patients with Sepsis

Antibacterial, proangiogenic, and osteopromotive nanoglass paste coordinates regenerative process following bacterial infection in hard tissue

Etiological structure of pig colibacillosis on the territory of the Russian Federation

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