Assessment of hospital daily cleaning practices using ATP bioluminescence in a developing country

Zambrano, Alejandra A.; Jones, Alex; Otero, Paula; Ajenjo, M. Cristina; Labarca, Jaime

doi:10.1016/j.bjid.2014.06.008

Cited by 16 publications

(19 citation statements)

References 5 publications

(18 reference statements)

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“…29 Surfaces in surgical ICUs were more likely to be contaminated with MRSA and VRE and particular attention is needed to improve cleaning at these units. 30 In partial agreement with our results Boyce et al 3 found that after cleaning of 100 high touched surfaces, 24% were still contaminated with MrSa, and 16% still yielded VRE. While Goodman et al 31 reported that environmental culture positive for MRSA or VRE were 45% before educational intervention versus 27% after educational intervention.…”

Section: Standard Precautions Compliance In Icussupporting

confidence: 92%

“…Significant improvements were obtained after educational intervention as 97.45% of surfaces became clean. These results are in agreement with those of Zambrano et al 30 who evaluated environmental cleaning of 198 hospital surfaces by aTP bioluminescence and not for citation purposes…”

supporting

confidence: 92%

“…28,29 These organisms can contaminate hands of HCWs when touching these surfaces without touching colonized or infected patient. 30,31 Strategies to reduce the rates of HaI with these pathogens should be established. 28,29 So we screened 118 frequently touched surfaces by aTP bioluminescence assay and found that most of the surfaces were contaminated with exception of incubator edges.…”

mentioning

confidence: 99%

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The fundamental role of educational intervention on improving health care workers’ knowledge, attitude and practice towards infection control precautions

Bassyouni¹,

Wegdan²,

El-Sherbiny³

2016

Int. j. infect. control

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To evaluate the role of educational intervention on health care workers' (HCWs) compliance to infection control precautions and cleaning of frequently touched surfaces at critical care units, forty-nine HCWs at 2 intensive care units (ICUs) and one neonatology unit at Fayoum University hospital were evaluated for knowledge, attitude and practice (KaP) towards standard precautions as well as obstacles affecting their compliance to standard precautions before and after a 32-hour purposed-designed infection control education program. a structured self-administrated questionnaire as well as observational checklists were used. assessment of Environmental cleaning was investigated by observational checklist, aTP bioluminescence and aerobic bacteriological culture for 118 frequently touched surfaces. Pre-intervention assessment revealed that 78.6% of HCWs were with good knowledge, 82.8% with good attitude and 80.8% had good practice. Obstacles identified by HCWs were as follow: making patient-care very technical (65.3%), deficiency of hand washing facilities (59.2%), skin irritation resulting from hand hygiene products (51%), and unavailability of PPE (38.8%). High significant improvements of knowledge, attitude and practice were detected after one month of educational intervention (P < 0.001). During the pre-interventional period only 30.5% of surfaces were considered clean versus 97.45% post intervention (P< 0.05). The highest Median ATP bioluminescence values were obtained from telephone handset, light switches and blood pressure cuffs. Staphylococcus aureus was the most commonly isolated organism followed by Enterococcus spp. and Escherichia coli (52, 38 and 19 surfaces respectively). In conclusion, training of HCWs on standard precautions should be considered a mandatory element in infection control programmes.

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Section: Standard Precautions Compliance In Icussupporting

confidence: 92%

supporting

confidence: 92%

mentioning

confidence: 99%

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The fundamental role of educational intervention on improving health care workers’ knowledge, attitude and practice towards infection control precautions

Bassyouni¹,

Wegdan²,

El-Sherbiny³

2016

Int. j. infect. control

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“…38 In recent years, several studies on the application of ATP-activity for monitoring microbial loads in environmental samples (including surface and device swabs, aqueous humidifier samples, and ready-toeat foods) were evaluated and was reported as a useful tool as it gave a good correlation with the standard methods. [39][40][41] The intervention program for reducing the microbial concentration in the air and on the surface areas in ambulances should emphasize the need for a ventilation improvement strategy, and improvement in the cleaning program of the ambulance environment, including medical instruments, seats, the floor, and air conditioning units. Additionally, the use of standard precautions, including hand hygiene and effective personal protective equipment (PPE) will prevent cross infection and save ambulance personnel's lives.…”

Section: Discussionmentioning

confidence: 99%

Microbial Air Quality and Bacterial Surface Contamination in Ambulances During Patient Services

Luksamijarulkul¹,

Pipitsangjan²

2015

Oman Med J

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This study revealed high microbial contamination (bacterial and fungal) in ambulance air during services and higher bacterial contamination on medical instrument surfaces and allocated areas after ambulance services compared to the start of ambulance runs. Additionally, bacterial and fungal counts in ambulance air showed a significantly positive correlation with the bacterial surface contamination on medical instruments and allocated areas. Further studies should be conducted to determine the optimal intervention to reduce microbial contamination in the ambulance environment.

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“…In addition, it is known that, after C&D procedures, many more surfaces considered as dirty by ACC are seen as clean by visual inspection when compared to another non-microbiological method (ATP-bioluminescence, fluorescent marker) (4,13) . Studies suggest that visual assessment is not sufficient to ensure the quality of the process, and it is less efficient than quantitative methods in raising awareness about the need to improve sanitation practices (20) . The use of ATP-bioluminescence to assess and have feedback of results leads to greater awareness, collaboration, communication and education of cleaning and nursing staffs, and therefore, it improves the efficiency of practices (21)(22) .…”

Section: Discussionmentioning

confidence: 99%

Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods

et al. 2017

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Approval: 02-02-2017 ABSTRACT Objective: to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit. Method: a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2.5 colony forming units per cm² were found in culture; when ≥5 relative light units per cm² were found at the ATP-bioluminescence assay. Results: 720 analyses were performed, 240 per method. The overall rates of clean surfaces per visual inspection, culture and ATP-bioluminescence assay were 8.3%, 20.8% and 44.2% before C&D, and 92.5%, 50% and 84.2% after C&D, respectively (p<0.001). There were only occasional statistically significant relationships between methods. Conclusion: the methods did not present a good correlation, neither quantitative nor qualitatively. Descriptors: Equipment Contamination; Housekeeping; Nursing Audit; Health Facility Environment; Infectious Disease Transmission. RESUMO Objetivo: avaliar a correlação do teste de ATP-bioluminescência com inspeção visual e cultura microbiológica na monitorização da eficiência da limpeza e desinfecção (L&D) de superfícies clínicas altamente tocadas (SCAT) em unidade de pronto atendimento. Métodos: estudo comparativo, prospectivo, conduzido de março a junho de 2015, de forma que cinco SCAT foram amostradas antes e depois da L&D de rotina pelos três métodos. As SCAT foram consideradas sujas quando apresentaram: na inspeção visual, poeira, dejetos, umidade e manchas; na cultura, ≥2,5 unidades formadoras de colônias por cm 2 e; no ATP-bioluminescência, ≥5 Unidades Relativas de Luz por cm 2 . Resultados: foram realizadas 720 avaliações, sendo 240 por método. A taxa global de superfícies limpas por inspeção visual, cultura e ATP-bioluminescência foi, respectivamente, de 8,3%, 20,8% e 44,2% antes da L&D e de 92,5%, 50% e 84,2% após (p<0,001). Houve apenas associações pontuais estatisticamente significativas entre os métodos. Conclusão: os métodos nem apresentaram boa correlação quantitativa, nem, qualitativa. Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods Frota OP, et al.

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Assessment of hospital daily cleaning practices using ATP bioluminescence in a developing country

Abstract: This study suggests that visual assessment is not enough to ensure quality of the process and it is necessary to document the level of cleanliness by quantitative methods.

Cited by 16 publications

References 5 publications

The fundamental role of educational intervention on improving health care workers’ knowledge, attitude and practice towards infection control precautions

The fundamental role of educational intervention on improving health care workers’ knowledge, attitude and practice towards infection control precautions

Microbial Air Quality and Bacterial Surface Contamination in Ambulances During Patient Services

Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods

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