Quantitative flow cytometric evaluation of CD200, CD123, CD43 and CD52 as a tool for the differential diagnosis of mature B-cell neoplasms

Abstract

BackgroundDistinction between mature B-cell neoplasms can be difficult due to overlapping of immunologic features and clinical manifestations. This study investigated whether quantifying mean fluorescence intensity of four monoclonal antibodies in a flow cytometry panel is useful for the differential diagnosis and characterization of these disorders.MethodsThe expressions of CD52, CD200, CD123 and CD43 were analyzed in samples from 124 patients with mature B-cell neoplasms. The quantitative estimation of these antigens was assessed by mean fluorescence intensity.ResultsThe cases included were 78 chronic lymphocytic leukemias, three atypical chronic lymphocytic leukemias, six marginal zone lymphomas, 11 splenic marginal zone lymphomas, nine lymphoplasmacytic lymphomas, six mantle cell lymphomas, two hairy cell leukemias, two hairy cell leukemias variant, five follicular lymphomas, one Burkitt lymphoma and one diffuse large B-cell lymphoma. The mean fluorescence intensity of CD200 was higher in atypical chronic lymphocytic leukemia, chronic lymphocytic leukemia and hairy cell leukemia cases. CD123 showed higher mean fluorescence intensities in hairy cell leukemia cells. Chronic lymphocytic leukemia, atypical chronic lymphocytic leukemia and mantle cell lymphoma had higher expression of CD43 and all follicular lymphoma cases had very low mean fluorescence intensity values. CD52 expression was consistently positive among all cases.ConclusionQuantitative evaluation of these markers can be a useful additional tool to better identify some types of mature B-cell neoplasms.

Superior Vena Cava Syndrome and Multiple Venous Thrombosis in a Patient with B Cell Lymphoma

“…The MBBHL is differentiated from DBBHL at the molecular level by the absence of Bcl-2. 11 On the other hand, presentation with SVCS is a rare condition in lymphomas, advanced cases may present this syndrome as seen in our patient.…”

Positive predictive value of CD200 positivity in the differential diagnosis of chronic lymphocytic leukemia

“…Forty‐three articles and meeting abstracts (Alapat et al, ; Arlindo, Marcondes, Fernandes, & Faulhaber, ; Aytan et al, ; Brunetti et al, ; Challagundla, Medeiros, Kanagal‐Shamanna, Miranda, & Jorgensen, ; D'Arena et al, ; Debord et al, ; Dorfman & Shahsafaei, ; El Desoukey, Afify, Amin, & Mohammed, ; El Din Fouad, Ibrahim, Abdel Aziz, & Ibrahim, ; Espinet et al, ; Falay et al, ; Fan et al, ; Fanoni et al, ; Favre et al, ; Hu et al, ; Köhnke et al, ; Lesesve et al, ; Mason, Pozdnyakova, Li, Dudley, & Dorfman, ; Miao et al, ; Miguet et al, ; Mongeau‐Marceau et al, ; Montesdeoca et al, ; Mora et al, ; Palumbo et al, ; Pillai et al, ; Poongodi, Varma, Naseem, Parveen, & Varma, ; Rahman et al, ; Rahman, Kumar, Gupta, Singh, & Nityanand, ; Rawstron et al, ; Rawstron et al, ; Rawstron et al, ; Sallam, Elsalakawy, El‐Sewefy, & Khattab, ; Sandes et al, ; Sorigue, Franch‐Sarto, Sarrate, & Junca, ; Sorigue, Juncà, et al, ; Sorigue, Junca, & Granada, ; Sorigue, Raya, et al, ; Spacek et al, ; Starostka et al, ; Ting et al, ; van Dongen et al, ; Ye et al, ) were read in full, of which 16 were excluded (Figure S1). Therefore, 27 studies (5,764 patients) were included.…”

Expression of neutrophil surface markers in icteric neonates before and after phototherapy

“…Flow cytometry immunophenotyping procedures were performed according to the following protocol : 50 μL of whole blood samples anticoagulated with EDTA were incubated with the fluorochrome‐conjugated monoclonal antibodies (MoAbs) described in Table for 20 min in the dark at room temperature. Red blood cells were lysed by incubation with Excellyse I, followed by incubation with distilled water.…”