DNA probe functionalized QCM biosensor based on gold nanoparticle amplification for Bacillus anthracis detection

Hao, Rongzhang; Song, Hongbin; Zuo, Guomin; Yang, Ruifu; Wei, Hongping; Wang, Dianbing; Cui, Zongqiang; Zhang, Zhiping; Cheng, Zhenxing; Zhang, Xian-En

doi:10.1016/j.bios.2011.01.010

Cited by 120 publications

(52 citation statements)

References 25 publications

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“…The benefit of increased sensitivity was also reported by Hao et al 24 in detection of Bacillus anthracis, which is the cause of serious bacterial infection, anthrax, 25 using quartz crystal microbalance (QCM). Thiolated DNA probe was immobilized onto the gold surface of QCM via self-assembly.…”

mentioning

confidence: 66%

Nanomaterial-based biosensors for biological detections

Su¹,

Li²,

Jin³

et al. 2017

AHCT

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Nanomaterial-based biosensors have become one of the major topics in the field of diagnostics. With the growing demand on devices with improved sensitivity and selectivity, rapid response time, and low cost, four categories of nanomaterials have become popular in biosensor research: gold nanoparticles, graphene, carbon nanotubes, and photonic crystals. The ongoing research has brought new designs of biosensors based on nanomaterials, which have greatly improved the potential of field-deployable microfabricated devices. This review describes the recent technologies employing the aforementioned nanomaterials for electrochemical detection of biomolecules, including glucose, DNA, protein, toxins, and so on. We envisage that miniaturized lab-on-a-chip devices employing these nanomaterials will soon be an essential part of our daily life.

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mentioning

confidence: 66%

Nanomaterial-based biosensors for biological detections

Su¹,

Li²,

Jin³

et al. 2017

AHCT

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“…This scheme was capable of detecting bacteria without sample enrichment showing an LOD of 2.0 Â 10 3 CFU/ mL. Recently, Hao et al developed this method to detect Bacillus anthracis based on the recognition of a 168-bp fragment of the Ba813 gene and the 340-bp fragment of the pag gene in plasmid pXO1 [74]. A thiol DNA probe was immobilized onto the QCM gold surface to hybridize to the target ssDNA obtained by asymmetric PCR.…”

Section: Piezoelectric Sensors Using Aunps For Dna/rna Recognitionmentioning

confidence: 98%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their molecular characterization, genomic sequences associated with medical conditions (including cancer), mutation and polymorphism identification, and the quantification of gene expression.

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“…Both electrochemical, gravimetric and optical sensors have been developed employing FNAs as receptors [130,[140][141][142][143][144][145][146][147].…”

Section: Dna Nanobiosensorsmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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DNA probe functionalized QCM biosensor based on gold nanoparticle amplification for Bacillus anthracis detection

Cited by 120 publications

References 25 publications

Nanomaterial-based biosensors for biological detections

Nanomaterial-based biosensors for biological detections

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Electrospinning-Based Nanobiosensors

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