Electrochemically deposited chitosan hydrogel for horseradish peroxidase immobilization through gold nanoparticles self-assembly

Liu, Xiang; Xu, Jing‐Juan; Zhang, Qing; Yang, Gongjun; Chen, Hong‐Yuan

doi:10.1016/j.bios.2004.07.029

Cited by 259 publications

(78 citation statements)

References 39 publications

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“…Because of its pH-responsive properties, chitosan electrodeposits onto a negatively biased electrode surface where local high pH is created. [10][11][12][13][14][15][16] Second, tyrosinase activates accessible tyrosine residues of the C-terminal pentatyrosine pro-tag into reactive o-quinones, which then covalently link to the nucleophilic amine groups of chitosan to form the protein-chitosan conjugate. [16][17][18][19][20] Chitosan confers its pH-responsive properties to the protein upon covalent conjugation.…”

Section: Introductionmentioning

confidence: 99%

Towards area‐based in vitro metabolic engineering: Assembly of Pfs enzyme onto patterned microfabricated chips

Lewandowski

Bentley

et al. 2008

Biotechnology Progress

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in Wiley InterScience (www.interscience.wiley.com).We report an approach for spatially selective assembly of an enzyme onto selected patterns of microfabricated chips. Our approach is based on electrodeposition of the aminopolysaccharide chitosan onto selected electrode patterns and covalent conjugation of a target enzyme to chitosan upon biochemical activation of a genetically fused ''pro-tag.'' We report assembly of S-adenosylhomocysteine nucleosidase (Pfs) fused with a C-terminal pentatyrosine pro-tag. Pfs is a member of the bacterial autoinducer-2 biosynthesis pathway, catalyzing the irreversible cleavage of S-adenosylhomocysteine. The assembled Pfs retains its catalytic activity and structure, as demonstrated by retained antibody recognition. Assembly is controlled by the electrode area, resulting in reproducible rates of catalytic conversion for a given area, and thus allowing for area-based manipulation of catalysis and small molecule biosynthesis. Our approach enables optimization of small molecule biosynthesis in 1-step as well as multistep enzymatic reactions, including entire metabolic pathways, and we envision a wide variety of potential applications.

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Section: Introductionmentioning

confidence: 99%

Towards area‐based in vitro metabolic engineering: Assembly of Pfs enzyme onto patterned microfabricated chips

Lewandowski

Bentley

et al. 2008

Biotechnology Progress

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“…The sensor was fabricated by immobilization of genetically engineered periplasmic glucose receptors to the GNPs, and showed selective detection of glucose in the micromolar concentration range, with a detection limit of 0.18 μM [82]. Many similar studies have been reported for the construction of biosensors based on the immobilization of different proteins with GNPs, such as horseradish peroxidase [62,83], microperoxidase-11 [84], tyrosinase [85] and human serum albumin [86]. Some biosensing elements are directly linked to the nanoparticles by the use of sulfur atoms within the bioentity [82], some are attached to the gold surface via thiol linkers [87,88], and some are immobilized through covalent bonds [89], or amine groups [90].…”

Section: Gnps Act As Immobilization Platformmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

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“…Yapısındaki -OH ve -NH 2 gibi aktif gruplar nedeniyle metallerle etkileşimi yüksek olan kitosanın soy metallerle ve geçiş metalleriyle kompozitleri elde edilmektedir [18][19][20].…”

Section: Introductionunclassified

Kitosan Destekli Au Nanopartiküller ile Modifiye Edilmiş Alüminyum Elektrotta Hidrojen Gazı Üretimi

Kayan¹,

Ilhan²,

Koçak³

2017

Çukurova Üniversitesi Mühendislik-Mimarlık Fakültesi Dergisi

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ÖzGünümüzün en büyük problemlerinden biri, enerji gereksiniminin karşılanmasında çok büyük oranda fosil yakıtların kullanılmasıdır. Artan enerji ihtiyacının fosil yakıtlardan sağlanması, çevresel problemlere ve enerji maliyetinin artmasına neden olmaktadır. Bu problemlerin çözümü ancak sürdürülebilir enerji kaynakları kullanımıyla gerçekleştirilebilecektir. Bu yüzden bol miktarda bulunan hidrojen, temiz ve ideal bir yakıt olarak geleceğin enerji kaynağı olarak düşünülmektedir. Hidrojen gazı üretiminin en basit yolu suyun elektrolizidir. Suyun elektrolizi ile hidrojen üretiminde karşılaşılan en büyük problem, elektrokimyasal sistemde oluşan aşırı gerilim nedeniyle maliyet artışıdır. Bu çalışmada suyun elektrolizi ile hidrojen elde etmek amacıyla ucuz bir materyal olarak seçilen alüminyum elektrodun yüzeyi, önce polianilin (PAni)-Kitosan kompozit yapısıyla modifiye edilmiş, daha sonra elde edilen bu kompozit yapı üzerine hidrojen oluşumunda yüksek katalitik aktivite gösteren altın (Au) nanopartiküller ayrıştırılmıştır. Elde edilen tüm yapıların yüzey özellikleri incelenmiş ve elektrokimyasal hidrojen üretimindeki katalitik aktiviteleri çeşitli elektrokimyasal yöntemler kullanılarak karşılaştırılmıştır.Anahtar Kelimeler: Hidrojen enerjisi, Elektrokataliz, İletken polimer, Kitosan, Au nanopartikül Hydrogen Gas Production on Aluminium Electrode Modified by ChitosanSupported with Au Nanoparticles AbstractOne of the major problems of today"s world is in its heavy dependency on fossil fuels for energy requirements. The increase in energy requirements causes environmental problems and leads to an increase in energy costs. It has already been obvious that the solution to these problems is to use of sustainable energy resources. Since hydrogen, the most abundant element is the cleanest and ideal fuel, has been considered as the fuel of the future. The simplest way of hydrogen production is the electrolysis of water. The major problem in the electrolysis of water for the hydrogen evolution is the increase in costs due to overvoltage of the electrochemical system. In this study, first the surface of the aluminium electrode as a cheap material, coated with polyanilin (PAni)-Chitosan composite film, then the gold (Au) nanoparticles electrodeposited on this composite surface which show high catalytic activity toward hydrogen production. The surface properties of the all obtained structures are investigated and the catalytic activities of the structures are compared with various electrochemical techniques for the electrocatalytic hydrogen production.

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Electrochemically deposited chitosan hydrogel for horseradish peroxidase immobilization through gold nanoparticles self-assembly

Cited by 259 publications

References 39 publications

Towards area‐based in vitro metabolic engineering: Assembly of Pfs enzyme onto patterned microfabricated chips

Towards area‐based in vitro metabolic engineering: Assembly of Pfs enzyme onto patterned microfabricated chips

Gold nanoparticle-based biosensors

Kitosan Destekli Au Nanopartiküller ile Modifiye Edilmiş Alüminyum Elektrotta Hidrojen Gazı Üretimi

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