Oxidative damage induced by copper in testis of the red swamp crayfish Procambarus clarkii and its underlying mechanisms

Zhao, Dan; Zhang, Xiaona; Li, Xuefu; Ru, Shaoguo; Wang, Yanwen; Yin, Jinbin; Liu, Dasheng

doi:10.1016/j.aquatox.2018.12.006

Cited by 34 publications

(18 citation statements)

References 60 publications

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“…However, in this study, we found a significant (negative) relationship only for Cu. Although high Cu concentrations were found to be detrimental in P. clarkii (Bini and Chelazzi 2006;Zhao et al 2019a), muscle has a slow Cu uptake rate compared to other tissues (Soedarini et al 2012) and Cu elimination seems to be quite efficient in this species (Zhao et al 2019b), resulting in low accumulation after exposure (Maranhão et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Metal accumulation in relation to size and body condition in an all-alien species community

Balzani

Kouba

Tricarico

et al. 2021

Environ Sci Pollut Res

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Metal pollution is one of the main environmental threats in freshwater ecosystems. Aquatic animals can accumulate these substances and transfer them across the food web, posing risks for both predators and humans. Accumulation patterns strongly vary depending on the location, species, and size (which in fish and crayfish is related to age) of individuals. Moreover, high metal concentrations can negatively affect animals’ health. To assess the intraspecific relationship between metal accumulation and size and health (proxied by the body condition) of individuals, the concentration of 14 metals (Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Ni, Pb, Se, Zn) was analyzed in six alien species from the highly anthropogenically altered Arno River (Central Italy): five fish (Alburnus alburnus, Pseudorasbora parva, Lepomis gibbosus, Ictalurus punctatus, and Silurus glanis) and one crayfish (Procambarus clarkii). We found that in P. clarkii, Cu was negatively related to size, as well as Al in L. gibbosus and Mg for adult I. punctatus. Positive size-dependent relationships were found for Hg in L. gibbosus, Fe in S. glanis, and Cr in juvenile I. punctatus. Only Co and Mg in S. glanis were found to negatively correlate with individual health. Since metal concentrations in animal tissue depend on trade-offs between uptake and excretion, the few significant results suggest different types of trade-offs across different species and age classes. However, only predatory fish species (L. gibbosus, I. punctatus, and S. glanis) presented significant relationships, suggesting that feeding habits are one of the primary drivers of metal accumulation.

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Section: Discussionmentioning

confidence: 99%

Metal accumulation in relation to size and body condition in an all-alien species community

Balzani

Kouba

Tricarico

et al. 2021

Environ Sci Pollut Res

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“…The testis serves as the site of spermatogenesis but is vulnerable to oxidative damage due to the presence of large amount of highly unsaturated fatty acids and the presence of ROS Aitken and Roman ( 2008 ). Some studies indicated that the Cu could cause decrease in the antioxidant capacity and testicular spermatogenesis (Zhao et al 2019 ; Zebral et al 2019 ; Lin et al 2020 ). Furthermore, Cu exposure upregulates the expression of genes of oxidative phosphorylation pathway (such as Cytc, SOD1, and GST) in the testis to induce ROS production, which is related with the toxicity of Cu in the testis (Zhao et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

“…Some studies indicated that the Cu could cause decrease in the antioxidant capacity and testicular spermatogenesis (Zhao et al 2019 ; Zebral et al 2019 ; Lin et al 2020 ). Furthermore, Cu exposure upregulates the expression of genes of oxidative phosphorylation pathway (such as Cytc, SOD1, and GST) in the testis to induce ROS production, which is related with the toxicity of Cu in the testis (Zhao et al 2019 ). These results reflect the disruption of balance between antioxidant and oxidant after Cu exposure, and it causes oxidative damage in the testis.…”

Section: Introductionmentioning

confidence: 99%

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis

Chen

Liao

et al. 2021

Environ Sci Pollut Res

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Copper (Cu) is a heavy metal which is being used widely in the industry and agriculture. However, the overuse of Cu makes it a common environmental pollutant. In order to investigate the testicular toxicity of Cu, the pigs were divided into three groups and were given Cu at 10 (control), 125, and 250 mg/kg body weight, respectively. The feeding period was 80 days. Serum hormone results showed that Cu exposure decreased the concentrations of follicular stimulating hormone (FSH) and luteinizing hormone (LH) and increased the concentration of thyroxine (T4). Meanwhile, Cu exposure upregulated the expression of Cu transporter mRNA (Slc31a1, ATP7A, and ATP7B) in the testis, leading to increase in testicular Cu and led to spermatogenesis disorder. The Cu exposure led to an increased expression of antioxidant-related mRNA (Gpx4, TRX, HO-1, SOD1, SOD2, SOD3, CAT), along with increase in the MDA concentration in the testis. In LG group, the ROS in the testis was significantly increased. Furthermore, the apoptotic-related mRNA (Caspase3, Caspase8, Caspase9, Bax, Cytc, Bak1, APAF1, p53) and protein (Active Caspase3) and the autophagy-related mRNA (Beclin1, ATG5, LC3, and LC3B) expression increased after Cu exposure. The mitochondrial membrane potential in the testicular tissue decreased, while the number of apoptotic cells increased, as a result of oxidative stress. Overall, our study indicated that the Cu exposure promotes testicular apoptosis and autophagy by mediating oxidative stress, which is considered as the key mechanism causing testicular degeneration as well as dysfunction.

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“…Furthermore, the price of crayfish is cheaper than that of other shrimp since it can be cultured in rice-crayfishbased coculture breeding modes, which reduces the cost of breeding. Therefore, crayfish have been increasingly favored by consumers [12]. In recent years, Hubei has become the largest crayfish-producing province in China, especially in the middle reaches of the Yangtze River [13].…”

Section: Introductionmentioning

confidence: 99%

Investigation of Bioaccumulation and Human Health Risk Assessment of Heavy Metals in Crayfish (Procambarus clarkii) Farming with a Rice-Crayfish-Based Coculture Breeding Modes

Peng

Gong

et al. 2022

Foods

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Due to the rapid development of the crayfish (Procambarus clarkii) industry in Chinese catering, people are paying more attention to the bioaccumulation of heavy metals in crayfish. To evaluate the health risks associated with the consumption of crayfish, nine types of heavy metals in both crayfish and abdominal muscles of crayfish were investigated. Crayfish samples were collected from rice-crayfish-based coculture breeding modes from different areas located in the middle and lower reaches of the Yangtze River. The average concentrations of heavy metals in the whole crayfish were much higher than the abdominal muscle of crayfish. The estimated daily intake (EDI) of heavy metals in the abdomen of crayfish was calculated to assess the noncarcinogenic risk and the overall noncarcinogenic risk including the target hazard quotient (THQ), the hazard index (HI) and carcinogenic risk (CR). The results of the present study showed that the consumption of crayfish may not present an obvious health risk to human associated with heavy metals. However, the THQ values of As in the abdominal muscles of crayfish for adults in EnShi (ES) and children in JiaYu (JY) should be of concern due to the higher contribution to the potential health risks of crayfish compared to other metals. Through X-ray photoelectron spectroscopy (XPS) detection of heavy metal As, it is found that As in crayfish culture environment mainly exists in the form of As3+.Therefore, the quality and quantity of crayfish consumption should be moderated to prevent the bioaccumulation of As. The results indicate that crayfish cultured in different areas may have similar pollution levels and/or emissions from the same pollution sources.

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Oxidative damage induced by copper in testis of the red swamp crayfish Procambarus clarkii and its underlying mechanisms

Cited by 34 publications

References 60 publications

Metal accumulation in relation to size and body condition in an all-alien species community

Metal accumulation in relation to size and body condition in an all-alien species community

Long-term copper exposure promotes apoptosis and autophagy by inducing oxidative stress in pig testis

Investigation of Bioaccumulation and Human Health Risk Assessment of Heavy Metals in Crayfish (Procambarus clarkii) Farming with a Rice-Crayfish-Based Coculture Breeding Modes

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