Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis

Wang, Qiangwei; Liang, Kang; Liu, Jingfu; Yang, Lihua; Guo, Yongyong; Liu, Chunsheng; Zhou, Bingsheng

doi:10.1016/j.aquatox.2012.11.009

Cited by 247 publications

(167 citation statements)

References 38 publications

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“…The result of the TDCPP agonistic activity towards the TR was consistent with those of several previously reported in vivo studies, which demonstrated that exposure to TDCPP could up-regulate the transcription of TR mediated genes in zebrafish embryos/larvae (Liu et al, 2013a;Wang et al, 2013). However, our results were different from those of a previously reported in vitro study, which showed that several OPEs, including TDCPP, had no TR activity (Kojima et al, 2013).…”

Section: Discussioncontrasting

confidence: 56%

“…Moreover, the results of numerous animal experiments have indicated the disruptive effect of OPEs on the thyroid endocrine system. Wang et al (2013Wang et al ( , 2015aWang et al ( , 2015b found that TDCPP changed TH levels and altered the transcription of genes involved in the hypothalamic-pituitary-thyroid (HPT) axis in zebrafish embryos/larvae. Liu et al (2013a) evaluated the effects of TDCPP and TPP on six receptor-associated expression of mRNA in zebrafish embryos/ larvae, and the results showed that TH receptor-centered gene networks were altered by OPEs.…”

Section: J O U R N a L O F E N V I R O N M E N T A L S C I E N C E Smentioning

confidence: 99%

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In vitro assessment of thyroid hormone receptor activity of four organophosphate esters

Ren

Cao

Yang

et al. 2016

Journal of Environmental Sciences

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Previous animal experiments have implied that organophosphate esters (OPEs) have a disruption effect on the thyroid endocrine system. However, knowledge of the toxicological mechanism remains limited. In this study, the activities of four OPEs have been characterized against the thyroid hormone (TH) nuclear receptor (TR) using two in vitro models, with the aim of evaluating their toxicity mechanisms towards the TR. The results of a TH-dependent cell proliferation assay showed that tris(2-chloro-1-(chloromethyl)ethyl)phosphate (TDCPP) could induce cell growth, while the other three OPEs had no effect. The results of a luciferase reporter gene assay revealed that all four of the OPEs tested in the current study showed agonistic activity towards TRβ, with TDCPP being the most potent one. Moreover, molecular docking revealed that all the tested OPEs could fit into the ligand binding pocket of TRβ, with TDCPP binding more effectively than the other three OPEs. Taken together, these data suggest that OPEs might disrupt the thyroid endocrine system via a mechanism involving the activation of TR.

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Section: Discussioncontrasting

confidence: 56%

Section: J O U R N a L O F E N V I R O N M E N T A L S C I E N C E Smentioning

confidence: 99%

In vitro assessment of thyroid hormone receptor activity of four organophosphate esters

Ren

Cao

Yang

et al. 2016

Journal of Environmental Sciences

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“…In vertebrates, expression of slc5a5 and tg is regulated by nkx2.1 (Zoeller et al, 2007). Many cases show that upregulated nkx2.1 level is associated with an increase of slc5a5 and tg (Yu et al, 2010;Wang et al, 2013b). However, in this study, there was a general up-regulation of nkx2.1and tg following BUVSs exposure, while slc5a5 was down-regulated after UV-234 exposure.…”

Section: Discussioncontrasting

confidence: 53%

Benzotriazole ultraviolet stabilizers alter the expression of the thyroid hormone pathway in zebrafish (Danio rerio) embryos

Liang

Martyniuk

et al. 2017

Chemosphere

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h i g h l i g h t sBUVSs decreased heart rate of zebrafish in all treatments by 6.9%e21.4%. BUVSs affected transcriptional levels of HPT axis-related genes. Disruption of thyroid-related transcripts was dependent on the structure of BUVSs. Gene networks and pathways were predicted using IMP. a r t i c l e i n f o a b s t r a c tBenzotriazole ultraviolet stabilizers (BUVSs) are widely used in industrial products as well as personalhygiene products to protect the material or skin from harmful UV-radiation. Due to their persistence and bioaccumulation, BUVSs have been ubiquitously detected in aquatic environments. Although the toxicological effects of BUVSs in aquatic organisms have been previously examined, the effects of BUVSs on the thyroid system have not been adequately addressed. In this study, we assessed putative thyroid disrupting effects of BUVSs (UV-234, UV-326, UV-329 and UV-P) in zebrafish embryos at 1, 10 and 100 mg/L for 96 h. The heart rate was assessed in zebrafish and was observed to be decreased by 6.9% e21.4% in exposure of tested BUVSs. We also observed that the transcript levels of HPT axis-related genes were affected by the 4 BUVSs tested in different ways. Specifically, mRNA levels of thyroid hormone receptors (thraa and thrb) in zebrafish embryos were differentially expressed and the direction of change in these transcripts was isoform and BUVSs dependent. Pathway analysis of the targeted genes measured indicated that cellular processes putatively affected by BUVSs included response to organic substance, regulation of transcription from RNA polymerase II promoter, intracellular receptor signaling pathway, and hypothyroidism. Upon expansion of the network, novel genes involved in this predicted gene network may provide insight into the mechanisms of thyroid disrupting mechanisms of BUVSs. Taken together, our results indicate that BUVSs can potentially impact the thyroid system, and that this is dependent upon the type or structure of BUVSs.

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“…Previous studies have demonstrated that several OP esters cause developmental toxicity and endocrine disruption in vivo and in vitro (Crump et al, 2012;Dishaw et al, 2012;Farhat et al, 2013;Fu et al, 2013;Liu et al, 2012bLiu et al, , 2013McGee et al, 2012McGee et al, , 2013Wang et al, 2013). For example, exposure of zebrafish embryos to tris(1,3-dichloro-2-propyl) phosphate (TDCPP) disrupted embryogenesis and caused larval malformation, with median lethal concentrations (LC 50 ) ranging from 3.7 to 7.0 mg/L (Fu et al, 2013;Liu et al, 2013;McGee et al, 2012;Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…For example, exposure of zebrafish embryos to tris(1,3-dichloro-2-propyl) phosphate (TDCPP) disrupted embryogenesis and caused larval malformation, with median lethal concentrations (LC 50 ) ranging from 3.7 to 7.0 mg/L (Fu et al, 2013;Liu et al, 2013;McGee et al, 2012;Wang et al, 2013). In chicken embryos, Farhat et al (2013) reported that injection of TDCPP or tris(1-chloro-2-propyl) phosphate (TCPP) delayed pipping, reduced tarsus length, and changed hormone concentrations and mRNA levels included in xenobiotic metabolism and thyroid hormone pathways.…”

Section: Introductionmentioning

confidence: 99%

Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate

Han

Wang

et al. 2014

Aquatic Toxicology

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Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis

Cited by 247 publications

References 38 publications

In vitro assessment of thyroid hormone receptor activity of four organophosphate esters

In vitro assessment of thyroid hormone receptor activity of four organophosphate esters

Benzotriazole ultraviolet stabilizers alter the expression of the thyroid hormone pathway in zebrafish (Danio rerio) embryos

Multiple bio-analytical methods to reveal possible molecular mechanisms of developmental toxicity in zebrafish embryos/larvae exposed to tris(2-butoxyethyl) phosphate

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