Decreased production of reactive oxygen intermediates is an early event during in vitro apoptosis of rat thymocytes

Wang, Jifeng; Jerrells, Thomas R.; Spitzer, John J.

doi:10.1016/0891-5849(95)02085-3

Cited by 66 publications

(39 citation statements)

References 29 publications

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“…Effective signaling concentrations of ROS may be below current detection limits. This could explain the decrease 50 or a lack of change 20 ). Cells appear to adapt to the same challenge using cell-type specific mechanisms and can show multiple mechanisms of resistance, some of which appear to be concentration-dependent.…”

Section: Discussionmentioning

confidence: 99%

Thymocytes selected for resistance to hydrogen peroxide show altered antioxidant enzyme profiles and resistance to dexamethasone-induced apoptosis

Tome

Briehl

2001

Cell Death Differ

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Treatment of WEHI7.2 cells, a mouse thymoma-derived cell line, with dexamethasone, a synthetic glucocorticoid, causes the cells to undergo apoptosis. Previous work has shown that treatment of WEHI7.2 cells with dexamethasone results in a downregulation of antioxidant defense enzymes, suggesting that increased oxidative stress may play a role in glucocorticoid-induced apoptosis. To test whether resistance to oxidative stress causes resistance to dexamethasoneinduced apoptosis, WEHI7.2 cell variants selected for resistance to 50, 100 and 200 mM H 2 O 2 were developed. Resistance to H 2 O 2 is accompanied by increased antioxidant enzyme activity, resistance to other oxidants and a delayed loss of viable cells after dexamethasone treatment. In the 200 mM H 2 O 2 -resistant cell variant the delay in cell loss is correlated with delayed release of cytochrome c from the mitochondria into the cytosol. This suggests that reactive oxygen species play a role in a signaling event during steroidmediated apoptosis in lymphocytes. Cell Death and Differentiation (2001) 8, 953 ± 961.

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Section: Discussionmentioning

confidence: 99%

Thymocytes selected for resistance to hydrogen peroxide show altered antioxidant enzyme profiles and resistance to dexamethasone-induced apoptosis

Tome

Briehl

2001

Cell Death Differ

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“…However, this finding combined with our previous observation that SV infection leads to increased intracellular glutathione levels (36) suggests that SV may activate the apoptotic pathway by perturbing the redox equilibrium toward a reduced state rather than an oxidized state. Of note, a decreased production of ROS has also been shown to be an early event in dexamethasoneinduced apoptosis in thymocytes (59), suggesting a more general role for "reductive stress" in activating cell death. What are the putative targets modified by decreased levels of intracellular O 2 .…”

Section: Increasing Intracellular Superoxide By Paraquat Protects Fromentioning

confidence: 99%

Decreased Intracellular Superoxide Levels Activate Sindbis Virus-induced Apoptosis

Lin¹,

Pasinelli²,

Brown³

et al. 1999

Journal of Biological Chemistry

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“…It has been shown that after physical disruption of cell contacts, apoptosis of thymocytes is induced during in vitro cultivation [27]. The present study showed that apoptosis of thymocytes from both LEC and WKAH rats was induced during in vitro cultivation and that the proportion of apoptotic cells from LEC rats was significantly higher than that from WKAH rats at each incubation time.…”

Section: Discussionmentioning

confidence: 48%

“…The mechanism of induction of apoptosis of rat thymocytes without de novo protein synthesis during in vitro cultivation remains unclear at this time. It has been suggested that ROS is a mediator of thymocyte apoptosis induced by in vitro cultivation [27]. Although ionizing radiation also generates ROS, radiationinduced apoptosis required de novo protein synthesis in rat thymocytes, but apoptosis by in vitro cultivation did not.…”

Section: Discussionmentioning

confidence: 99%

“…Although ionizing radiation also generates ROS, radiationinduced apoptosis required de novo protein synthesis in rat thymocytes, but apoptosis by in vitro cultivation did not. In the case of in vitro cultivation, the production of ROS in thymocytes is likely to originate mainly from mitochondria [27]. On the other hand, ionizing radiation induces apoptosis via nuclear DNA damage.…”

Section: Discussionmentioning

confidence: 99%

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High Sensitivity of Thymocytes of LEC Strain Rats to Induction of Apoptosis by X-Irradiation.

Hayashi

Nagata

Endoh

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. It is known that physical disruption of cell contacts induces apoptosis of thymocytes. When thymocytes from LEC and WKAH rats were incubated in vitro at 37°C for 0-6 hr and then the proportion of apoptotic cells was determined using a flow cytometer, it was found that the percentages of apoptotic thymocytes from both LEC and WKAH rats increased with incubation time and that the proportion of apoptotic cells from LEC rats was significantly higher than that from WKAH rats at each incubation time. The fact that cycloheximide, an inhibitor of protein synthesis, did not show significant inhibitory effects on induction of apoptosis of thymocytes indicates that induction of apoptosis during in vitro cultivation did not require de novo protein synthesis. When thymocytes from LEC and WKAH rats were X-irradiated in vitro at 4 and 8 Gy, the percentages of radiation-induced apoptotic cells increased with post-incubation time after X-irradiation in both LEC and WKAH rat thymocytes and the proportions of apoptotic cells from LEC rats were significa ntly higher than those from WKAH rat cells at 2 and 4 hr post-incubation after X-irradiation. When thymocytes from LEC and WKAH rats were X-irradiated in the presence of cycloheximide, the induction of apoptosis was substantially inhibited, indicating that radiationinduced apoptosis of thymocytes from LEC and WKAH rats required de novo protein synthesis. The present results showed high sensitivities of thymocytes of LEC rats to induction of apoptosis during in vitro cultivation and by X-irradiation. KEY WORDS: apoptosis, LEC strain rat, protein synthesis, thymocyte, X-irradiation.

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Decreased production of reactive oxygen intermediates is an early event during in vitro apoptosis of rat thymocytes

Cited by 66 publications

References 29 publications

Thymocytes selected for resistance to hydrogen peroxide show altered antioxidant enzyme profiles and resistance to dexamethasone-induced apoptosis

Thymocytes selected for resistance to hydrogen peroxide show altered antioxidant enzyme profiles and resistance to dexamethasone-induced apoptosis

Decreased Intracellular Superoxide Levels Activate Sindbis Virus-induced Apoptosis

High Sensitivity of Thymocytes of LEC Strain Rats to Induction of Apoptosis by X-Irradiation.

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