Separation and characterization of stroma and grana membranes — evidence for heterogeneity in antenna size of both Photosystem I and Photosystem II

Andreasson, Eva; Svensson, Per H.; Weibull, Claes; Albertsson, Per‐Åke

doi:10.1016/0005-2728(88)90010-2

Cited by 101 publications

(53 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When PhQ, two molecules of which exist per PS I, is taken as an index for the amount of PS I, the Chl a′/PhQ ratio of 0.58 supports the stoichiometry of one molecule of Chl a′ per PS I. However, comparisons of the Chl a′/Chl a ratio with the P700/Chl a ratios determined previously by several workers, 0.26% 30 to 0.34%, 31 lead to the stoichiometry of 1.32 to 1.74 molecules of Chl a′ per PS I. To reach a final conclusion as to the Chl a′/PS I stoichiometry, we need to conduct detailed pigment composition analysis of intact PS I preparations and to compare the results of spectrophotometric determination of P700.…”

Section: Pigment Composition Analysis Of Thylakoid Membranes and Lighmentioning

confidence: 73%

Separation and Determination of Minor Photosynthetic Pigments by Reversed-phase HPLC with Minimal Alteration of Chlorophylls

Nakamura

Watanabe

2001

ANAL. SCI.

View full text Add to dashboard Cite

Reversed-phase HPLC conditions for separation of chlorophyll (Chl) a, Chl a′ (the C13 2 -epimer of Chl a), pheophytin (Pheo) a (the primary electron acceptor of photosystem (PS) II), and phylloquinone (PhQ) (the secondary electron acceptor of PS I), have been developed. Pigment extraction conditions were optimized in terms of pigment alteration and extraction efficiency. Pigment composition analysis of light-harvesting complex II, which would not contain Chl a′ nor Pheo a, showed the Chl a′/Chl a ratio of 3 -4 × 10 -4 and the Pheo a/Chl a ratio of 4 -5 × 10 -4 , showing that the conditions developed here were sufficiently inert for Chl analysis. Preliminary analysis of thylakoid membranes with this analytical system gave the PhQ/Chl a′ ratio of 0.58 ± 0.03 (n = 4), in line with the stoichiometry of one molecule of Chl a′ per PS I.

show abstract

Section: Pigment Composition Analysis Of Thylakoid Membranes and Lighmentioning

confidence: 73%

Separation and Determination of Minor Photosynthetic Pigments by Reversed-phase HPLC with Minimal Alteration of Chlorophylls

Nakamura

Watanabe

2001

ANAL. SCI.

View full text Add to dashboard Cite

show abstract

“…To decide this alternative we have carried out phase partitioning of thylakoids (Andreasson et al, 1988) and isolation of photosystem I by Triton-X-100 treatment of the membranes (Wynn and Malkin, 1988). To locate ELIP in one or both of the photosystems, the leaves had to contain both photosystems and ELIP.…”

Section: Discussionmentioning

confidence: 99%

“…The separation of pea thylakoids in grana-enriched and stroma-enriched membrane fractions was carried out by phase partitioning of sonicated thylakoids, as described by Andreasson et al (1988). The pea seedlings used were grown for 6 days in the dark and illuminated subsequently for 2 days prior to harvesting.…”

Section: Separation Of Gruna and Stromn Thy1akoidsmentioning

confidence: 99%

“…4. For the D1 protein and photosystem I subunit I, there is a distribution in the two fractions as described elsewhere (Andreasson et al, 1988;Akerlund and Anderson, 1983) with an increased amount of the photosystem I protein in the T3 fraction and an increased amount of the photosystem I1 protein in the B3 fraction in relation to unfractionated thylakoids. ELlP is not distributed uniformly in the thylakoid membranes but is found in an increased amount in the stroma-thylakoid-enriched T3 fraction, as is the photosystem I proteins.…”

Section: Fractionation Q F' Thy Lrrko Idsmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of the early light‐inducible protein in the thylakoids of developing pea chloroplasts

Cronshagen

Herzfeld

1990

European Journal of Biochemistry

View full text Add to dashboard Cite

The distribution of the early light-inducible protein (ELIP) of pea (Pisum sativurn) between grana and stroma thylakoids was studied. An antibody raised against a bacterial-expressed fusion protein containing ELIP sequences was used.Illumination of dark-grown pea seedlings causes an accumulation of the ELIP in the thylakoid membranes with a maximum level at 16 h. During continuous illumination exceeding 16 h the level decreases again. The fractionation of thylakoid membranes of 48-h-illuminated pea seedlings in grana and stroma thylakoids reveals that there is no uniform distribution of ELIP in the thylakoids. Rather 60 -70% of ELIP was found in the stroma thylakoids and 30-40% in the grana thylakoids. This distribution is in accordance with that of photosystem 1 but not with that of photosystem 11. After Triton-X-100 solubilization almost all ELIP is found in the photosystem-I-containing fraction. This also supports an association of ELIP with photosystem I.

show abstract

“…The fractions f and a were prepared as described in ref. 3. The f nonappressed fraction has a Chl a/b ratio of 4.8, and the a appressed membrane fraction has a Chl a/b ratio of 2.4.…”

Section: Apoproteins Of Cp29 and Cp26 In Spinach Thylakoidsmentioning

confidence: 99%

Biochemical Characterization of Photosystem II Antenna Polypeptides in Grana and Stroma Membranes of Spinach

Allen¹,

Staehelin²

1992

Plant Physiol.

View full text Add to dashboard Cite

The photosystem (PS) II antenna system comprises several biochemically and spectroscopically distinct complexes, including light-harvesting complex 11 (LHCII), chlorophyll-protein complex (CP) 29, CP26, and CP24. LHCII, the most abundant of these, is both structurally and functionally diverse. The photosynthetic apparatus is laterally segregated within the thylakoid membrane into PSI-rich and PSII-rich domains, and the distribution of antenna complexes between these domains has implications for antenna function. We report a detailed analysis of the differences in the polypeptide composition of LHCII, CP29, and CP26 complexes associated with grana and stroma thylakoid fractions from spinach (Spinacia oleracea L.), making use of a very high-resolution denaturing gel system, coupled with immunoblots using monospecific antibodies to identify specific antenna components. We first show that the polypeptide composition of the PSII antenna system is more complex than previously thought. We resolved at least five type I LHCII apoproteins and two to three type 11 LHCII apoproteins. We also resolved at least two apoproteins each for CP29 and CP26. In state 1-adapted grana and stroma thylakoid membranes, the spectrum of LHCII apoproteins is surprisingly similar. However, in addition to overall quantitative differences, we saw subtle but reproducible qualitative differences in the spectrum of LHCII apoproteins in grana and stroma membrane domains, including two forms of the major type 11 apoprotein. The implications of these findings for models of PSII antenna function in spinach are discussed.Most of the Chl in the thylakoid membrane of higher plants and green algae is bound by specialized antenna complexes associated with either PSI or PSII (5,15,33 Chl. The CP29 complex has a Chl a/b ratio of 2.2 to 2.5 (5, 27) and has an apoprotein several kD larger than those of the LHCII complex. CP26 also has a higher Chl a/b ratio than LHCII but generally comigrates with LHCII in both native and fully denaturing SDS-PAGE systems (4, 27). Coupled with the difficulty in separating CP29 from the LHCII apoproteins in some species (particularly in spinach [16,17]), this has contributed to confusion in the literature as to the identities of these two complexes. This issue has recently been settled by the comparison of partial protein sequence data for CP29 and CP26 in barley (Hordeum vulgare) with a full-length gene sequence for CP29 (26), confirming that CP29 and CP26 are distinct complexes, both from each other and from LHCII.In this paper we will use the terms CP26 and CP29 as defined in ref. 4. Little is known for certain about the physical arrangement of the PSII antenna system, but the isolation of 02-evolving complexes lacking LHCII but still containing CP29 suggests that CP29 is more closely associated with the reaction center (9). The gel systems used in ref. 9 did not distinguish between CP29 and CP26; therefore, no conclusions could be drawn about the relationship of CP26 to the reaction center.

show abstract

Separation and characterization of stroma and grana membranes — evidence for heterogeneity in antenna size of both Photosystem I and Photosystem II

Cited by 101 publications

References 36 publications

Separation and Determination of Minor Photosynthetic Pigments by Reversed-phase HPLC with Minimal Alteration of Chlorophylls

Separation and Determination of Minor Photosynthetic Pigments by Reversed-phase HPLC with Minimal Alteration of Chlorophylls

Distribution of the early light‐inducible protein in the thylakoids of developing pea chloroplasts

Biochemical Characterization of Photosystem II Antenna Polypeptides in Grana and Stroma Membranes of Spinach

Contact Info

Product

Resources

About