Evaluation of a new transport medium for the preservation of oral streptococci

Rundell, Barbara B.; Thomson, L. A.; Loesche, Walter J.; Stiles, H. M.

doi:10.1016/0003-9969(73)90057-5

Cited by 26 publications

(22 citation statements)

References 13 publications

(12 reference statements)

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“…Half of the floss was passed between the premo lars and the other half between the molars. In this manner four floss samples con taining plaque removed from 16 approximal surfaces were collected and placed in 10 ml of a reduced transport fluid (RTF) [Rundell et al, 1973;Svf.d and Loesche, 1972]. Occlusal fissure samples were obtained from the second premolars and sec ond molars of each quadrant by means of a pointed wire.…”

Section: Methodsmentioning

confidence: 99%

Effect of Topical Acidulated Phosphate Fluoride on Percentage of Streptococcus mutans and Streptococcus sanguis in Plaque

Loesche¹,

Syed²,

Murray³

et al. 1975

Caries Res

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The percentage of Streptococcus mutans and Streptococcus sanguis in pooled occlusal plaque and in pooled approximal plaque following 8–10 daily applications of either an acidulated phosphate fluoride (APF) gel or a placebo gel was determined. S. mutans averaged about 9% of the colony forming units (CFU) in the occlusal plaque and 1% of the CFU in the approximal plaque prior to treatment. S. sanguis accounted for about 5% of the approximal isolates and 8% of the occlusal isolates prior to treatment. The APF treatment caused a persistent 45–75% reduction in the percentage of S. mutans in the occlusal samples but did not have an effect on the percentage of S. mutans in the approximal plaque. Great individual variability in the percentages of S. mutans prevented the demonstration of significant differences between the APF and placebo group when parametric statistical tests were employed. When ranking procedures were used, i.e. Kruskal Wallis, Willcoxon tests, the APF was found to have a specific antimicrobial effect against S. mutans in the occlusal plaque, but not in the approximal plaque. This effect was still apparent 12 weeks after the last treatment. There was no effect of the APF on the percentages of S. sanguis in either sample, except at one week after treatment, where the approximal samples showed a significant increase. The plaque and gingivitis scores in the APF group were significantly less than in the placebo group.

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Section: Methodsmentioning

confidence: 99%

Effect of Topical Acidulated Phosphate Fluoride on Percentage of Streptococcus mutans and Streptococcus sanguis in Plaque

Loesche¹,

Syed²,

Murray³

et al. 1975

Caries Res

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“…Sampling and cultivation.-Plaque was collected from the gingival margins and sulci of the indicated sites with a sterile curette under nitrogen flow, placed in reduced transport fluid (RTF) (Rundell et al, 1973), and immediately introduced into a nitrogen-containing glove-box (Plas-Labs., Plastics Manufacturing and Supply, Inc., Lansing, MI). The individual samples were dispersed on a Vortex Genie for ten s (Scientific Instruments, Inc., Bohemia, IL) and diluted serially three times with ten-fold dilutions of RTF.…”

Section: Methodsmentioning

confidence: 99%

Comparative Microbiological and Immunological Studies of Subgingival Dental Plaque from Man and Baboons

et al. 1990

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Baboons may be useful as animal models for the study of human oral diseases and infections. They are closely related to man anatomically, physiologically, and phylogenetically. Plaque and gingival indices were relatively low in 18 baboons (Papio anubis). The mean scores ranged between 0.62 +/- 0.29 and 0.37 +/- 0.20, respectively. Gram-positive and Gram-negative cocci comprised 27.0 +/- 32.4 and 3.1 +/- 7.5% of the total viable counts in the dental plaque samples. Black-pigmented Bacteroides formed about 1.9 +/- 5.9% of the bacterial population in the samples. Anaerobic Gram-negative bacilli were found in 73.2% of the samples and averaged 19.2 +/- 26.3% of the total recovered flora. Species of the oral Actinomyces and other Gram-positive rods found in humans were not isolated. The composition of the oral flora in baboons appeared to be significantly different from that of man. Isolates of F. nucleatum, L. buccalis, and B. intermedius from the two mammals were biochemically similar, but were distinguishable by analysis with antibody, both by precipitin lines and/or differences between homologous and heterologous titers.

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“…These strains are, amongst others, considered to be responsible for the development of caries and CLINICAL EFFECTS OF MOUTHRINSES 213 gingivitis. The plaque sample was suspended in a sterile transport fluid (1 ml) (RTF, Rundell et al, 1973) and within 2 h dispersed by sonication for 10 s at 70 W using a microprobe (Braun Labsonic 1510) and subsequently serially diluted. Appropriate dilutions were placed on the following selective and non selective media: MSB agar (Difco) for the enumeration of S. mutans, Rogosa agar (Difco) for lactobacilli, veillonella agar, consisting of trypton, yeast extract, agar supplemented with salts, 5% defibrinated sheep blood, 1 % lactic acid and 7-5 ug-ml~~ ' vancomycin, and CFAT agar and ETSA agar (Syed et al, 1980) were used for total anaerobic counts.…”

Section: Determination Of the Microbio! Composition Of The Plaquementioning

confidence: 99%

Clinical effects of commercially available mouthrinses on the development of plaque, gingivitis and enamel surface free energy

1991

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In this study the clinical effects of 6 commercially available mouthrinses on the development of plaque and gingivitis and on the tooth surface free energy were evaluated in vivo. The following rinses were used: Hibident ® (containing 0-2% chlorhexidine), Prodent ® (containing 005% sodium fluoride) Meridol ® (containing 125 ppm aminefluoride, 125 ppm stannous fluoride), Merocet ® (containing 0.05% cetylpyridinium chloride), Veadent ® (containing 0.03% sanguinarine) and Listerine ® (containing several phenolic compounds). Sixty test persons were selected and requested to employ the same, non-fluoridated toothpaste over 14 days. At days 0 and 14, the plaque index (PI), gingival index (GI), planimetric plaque index (PP) and the tooth surface free energy were assessed. After this preparatory phase, all oral hygiene was stopped for 6 days and only a rinse twice a day (30 s, 10 ml was used). After this period, the above parameters were measured again. In addition, the microbial composition of the plaque was determined at the beginning (day 0) and the end of the study (day 20). Hibident ® (incorporated in this study as a positive control) demonstrated the lowest PI after 6 days use, whereas Meridol ® demonstrated the lowest GI. PI and GI scores were highest after the use of Prodent ® (incorporated as a negative control). None of the products were able to alter the tooth surface free energy markedly or to cause great shifts in microbial composition of the plaque. Since in this study, 6 products were evaluated in an identical way, the results provide a rigorous basis for comparing their clinical efficacies.

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Evaluation of a new transport medium for the preservation of oral streptococci

Cited by 26 publications

References 13 publications

Effect of Topical Acidulated Phosphate Fluoride on Percentage of Streptococcus mutans and Streptococcus sanguis in Plaque

Effect of Topical Acidulated Phosphate Fluoride on Percentage of Streptococcus mutans and Streptococcus sanguis in Plaque

Comparative Microbiological and Immunological Studies of Subgingival Dental Plaque from Man and Baboons

Clinical effects of commercially available mouthrinses on the development of plaque, gingivitis and enamel surface free energy

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