Isolation and characterization of thermophilic bacterial strains from Soldhar (Tapovan) hot spring in Central Himalayan Region, India

Arya, Mamta; Joshi, Gopal; Gupta, Atul; Kumar, Anil; Raturi, Aparna

doi:10.1007/s13213-014-0984-y

Cited by 21 publications

(12 citation statements)

References 24 publications

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“…The number of thermophilic bacterial populations recovered was dependent on the media composition. Nutrient agar and Tryptic soy medium were employed for isolating a large proportion of the available diversity mainly the thermotolerant isolates; both media support the growth of bacteria from hot springs when incubated at high temperature [39]. Furthermore, Thermus medium, ATCC medium 697, and Thermus 162 medium were suitable for isolation of thermophilic bacteria, including novel species [63].…”

Section: Discussionmentioning

confidence: 99%

“…Two techniques, liquid enrichment and dilution plating, were used to isolate the thermophilic bacteria on the following six different culture media: Thermus medium [35], Castenholz medium D, Thermus 162 medium [36], ATCC medium 697 [37], Nutrient medium (Liofilchem) [38], and Tryptic soy medium (BD, Difco) [39]. Solid media were obtained by adding 3% (w/v) of agar to liquid media.…”

Section: Sample Collection and Isolation Of Thermophilic Bacteriamentioning

confidence: 99%

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Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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This study aims to determine the diversity of culturable thermophilic bacteria isolated from eight terrestrial hot springs in Northeastern of Algeria using the conventional methods, SDS-PAGE fingerprinting of whole-cell proteins and 16S rRNA gene sequencing. In addition, their hydrolytic enzyme activities were also investigated. A total of 293 strains were isolated from the hot springs' water and sediment using different culture media. Overall, five distinct bacterial groups were characterized by whole-cell protein pattern analysis. Based on the 16S rRNA gene sequencing of 100 selected strains, the isolates were assigned to the following three major phyla: Firmicutes (93%), Deinococcus-Thermus (5%), and Actinobacteria (2%), which included 27 distinct species belonging to 12 different phylotypes, Aeribacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Geobacillus, Laceyella, Meiothermus, Saccharomonospora, Thermoactinomyces, Thermobifida, and Thermus. The screening for nine extracellular enzymes showed that 65.87% of the isolates presented at least five types of enzyme activities, and 6.48% of strains combined all tested enzymes (amylase, cellulase, pectinase, esculinase, protease, gelatinase, lipase, lecithinase, and nuclease). It was found that Bacillus, Anoxybacillus, Aeribacillus, and Aneurinibacillus were the genera showing the highest activities. Likewise, the study showed an abundant and diverse thermophilic community with novel taxa presenting a promising source of thermozymes with important biotechnological applications. This study showed that a combined identification method using SDS-PAGE profiles of whole-cell proteins and subsequent 16S rRNA gene sequence analysis could successfully differentiate thermophilic bacteria from Algerian hot springs.

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Section: Discussionmentioning

confidence: 99%

Section: Sample Collection and Isolation Of Thermophilic Bacteriamentioning

confidence: 99%

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

et al. 2020

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“…The growing interest in thermophilic microorganisms and their potential biotechnological applications explains the increasing number of studies in extremophilic microorganisms around the world (Aanniz et al 2015;Adiguzel et al 2009;Arya et al 2015;Baltaci et al 2017;Kublanov et al 2009). The order Bacillales includes thermophilic members of the genera Geobacillus, Alicyclobacillus, Aneurinibacillus, Anoxybacillus, Bacillus, Brevibacillus, Caldalkalibacillus, Sulfobacillus, Thermobacillus, Ureibacillus and Vulcanibacillus and is responsible for the degradation of organic matter in hot environments (Logan and Allan 2008;Thebti et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Around the world, biodiversity and bioprospection of thermophilic microorganisms are topics of great interest (Arya et al 2015;Baltaci et al 2017;Kublanov et al 2009). However, in Argentinaespecially in relation to Copahue and Domuyo geothermal areasonly the microbial biodiversity of the acidic ponds and biofilms in the Copahue geothermal field has been studied (Chiacchiarini et al 2010;Urbieta et al 2014;Urbieta et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of Keratinase and Other Hydrolytic Enzymes in Thermophilic Bacteria Isolated from Geothermal Areas in Patagonia Argentina

Cavello

Urbieta

Segretin

et al. 2017

Geomicrobiology Journal

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Thermophilic aerobic bacteria were isolated from two geothermal areas in Neuqu en province using two different enrichment methods and a total of 30 isolates were obtained. From chicken feather enrichment cultures, strains affiliated to Bacillus cytotoxicus and Bacillus licheniformis were isolated and all of them demonstrated the capability to degrade completely chicken feather. A preliminary research on biotechnological enzymes' potential demonstrated that all the isolates displayed at least one of the extracellular hydrolytic enzymes tested. Most of the isolates showed protease, inulinase and/or pectinase activities, while cellulase and xylanase activities were less common. In light of these findings, geothermal areas of Argentina may be considered as a potential source of thermophilic bacteria able to produce many industrially relevant enzymes.

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“…Hot springs, namely Soldhar and Ringigad located in Chamoli district of Uttarakhand state in IHR, have been a focus for understanding the basic and applied aspects of the microbial diversity of thermophiles. These thermophilic organisms are important in view of their bioprospecting including their diversity, applications and conservation [6][7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

Dhyani

Gururani

Selim

et al. 2017

RIB

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Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.

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Isolation and characterization of thermophilic bacterial strains from Soldhar (Tapovan) hot spring in Central Himalayan Region, India

Cited by 21 publications

References 24 publications

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

Diversity and enzymatic potential of thermophilic bacteria associated with terrestrial hot springs in Algeria

Assessment of Keratinase and Other Hydrolytic Enzymes in Thermophilic Bacteria Isolated from Geothermal Areas in Patagonia Argentina

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

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