Improved in vitro shoot multiplication and rooting of Dendrocalamus hamiltonii Nees et Arn. Ex Munro: production of genetically uniform plants and field evaluation

Abstract

An efficient in vitro regeneration protocol and field performance of a multipurpose bamboo species Dendrocalamus hamiltonii Nees et Arn. Ex Munro has been demonstrated using single node cuttings taken from the lateral branches of a 20-year-old bush. Axillary buds on the nodal explant sprouted within 10 days of culture on Murashige and Skoog (MS) medium without any plant growth substance. High-frequency proliferation was induced on the propagules (small clusters with 3-5 multiple shoots and rhizomatous portions). Subsequent removal of the shoots (about 1.5 cm) from the rhizomatous portion of propagules (shoot cut) influenced the plantlet formation capacity. A multiplication of about 20-folds was achieved on MS medium supplemented with 8 lM BAP and 1 lM NAA. Rooting efficiency was also markedly enhanced ([90%) when the propagules, following shoot cut, were placed on to MS medium supplemented with 100 lM IBA for 10 days and then transferred to IBA-free medium. This is the first report from this species where 20-fold increment in multiplication was observed at the end of second subculture followed by [90% rooting. The hardened plants, established in the field, exhibited normal growth; their physiological performance has been monitored at 6-month intervals. The rate of photosynthesis increased from 3.55 lmol CO 2 m -2 s -1 (hardened, ready for field transfer) to 5.44 lmol m -2 s -1 (6 months of field transfer); following a year of plantation net photosynthesis recorded was 14.0 lmol CO 2 m -2 s -1 while after 1.5 years it was 12.76 lmol CO 2 m -2 s -1 . These values were compared with those observed for the mother bush. Genetic fidelity of these regenerants was established by RAPD analysis advocating clonal propagation of this species through nodal segment culture and its commercial cultivation.

Analysis of genetic stability of in vitro propagated potato microtubers using DNA markers

“…The present data are similar to reports of others. There are a number of findings in the literature which report similar results for detection of genetic fidelity using molecular markers in different crops such as Solanum aculeatissimum (Ghimire et al 2012), Olea europaea (Leva and Petruccelli 2012), Saccharum officinarum (Tawar et al 2008), Dendrocalamus hamiltonii (Agnihotri et al 2009), Capparis deciduas (Tyagi et al 2010), Vanilla spp. (Sreedhar et al 2007), Dioscorea bulbifera (Narula et al 2007) and Musa spp.…”

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

“…Likewise, Sood et al (1992); Bag et al (2000); Agnihotri et al (2009) and Nadha et al (2013) in other bamboo species, Arya et al (2012) in Dendrocalamus hamiltonii.…”

Testing of genetic homogeneity of elite eucalyptus clones using DNA marker

“…This also establishes the usefulness of ISSR marker system in ascertaining the genetic purity of clones. Earlier also, ISSR markers were successfully employed in bamboo species (Agnihotri et al 2009, Singh et al 2013, date palm (Kumar et al 2010), apple (Gupta et al 2009), jalamdasa (Chandrika & Rai 2009), acacia (Nanda et al 2004), stevia (Lata et al 2013), albizia (Mohammad et al 2016) and by many others.…”

“…Several strategies have been adopted for genetic purity testing. In past, morphological descriptions, physiological traits, cytological studies, isozymes (Gupta & Varshney 1999, Devarumath et al 2002, Agnihotri et al 2009, Singh et al 2012a, 2012b and many other techniques have been deployed to assess the genetic purity of clonally mass propagated plants. However, expression of the morphological and physiological traits may changes in response to the prevalent environmental conditions (Singh et al 2013).…”