Superparamagnetic poly(methyl methacrylate) nanoparticles surface modified with folic acid presenting cell uptake mediated by endocytosis

Feuser, Paulo Emílio; Jacques, Amanda Virtuoso; Arévalo, Juan Marcelo Carpio; Rocha, Maria Eliane Merlin; Santos-Silva, Maria Cláudia; Sayer, Cláudia; Araújo, Pedro Henrique Hermes de

doi:10.1007/s11051-016-3406-1

Cited by 14 publications

(7 citation statements)

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“…The total concentration of iron oxide obtained by the coprecipitation method was 55 %, as described by Feuser and collaborates [4]. In the case of lauryl gallate loaded in superparamagnetic PMMA nanoparticles surface modified with FA the mass loss is gradual and it can be clearly observed that the polymer is completely degraded when reaching a temperature about 430 °C [4,5,17].…”

Section: Characterization Of Lauryl Gallate Loaded In Superparamagnet...mentioning

confidence: 94%

“…This study was approved by the medical ethics committee of Polydoro Ernani de São Thiago University Hospital (Florianopolis, Brazil) as described previously [17]. Citratestabilized human blood was freshly collected and used within 3 h of being drawn.…”

Section: Hemolysis Assaymentioning

confidence: 99%

“…The internalization of carrier systems labeled with 6coumarin (MNPsG12PMMA and FA-MNPsG12PMMA) were evaluated with laser scanning confocal microscopy. HeLa cells (5 × 10 4 ) were cultured on 13 mm diameter glass coverslips in 24 well plates for 72 h. Cells were then treated with 10 µg/mL (lauryl gallate concentration) of lauryl gallate loaded in superparamagnetic PMMA nanoparticles labeled with 6-coumarin [17] with and without FA (in PBS), and incubated for 1 h, at 37 °C. In order to block the endocytotic (energy-dependent) pathways of lauryl gallate loaded in superparamagnetic PMMA nanoparticles with or without FA (dissolved in PBS) were also incubated for 1 h at 4 °C.…”

Section: Hemolysis Assaymentioning

confidence: 99%

“…However, these peaks were not observed in the diffractograms when the lauryl gallate was encapsulated in superparamagnetic PMMA nanoparticles. The peaks observed at 2θ: (220), (311), (400), ( 422), (511), and (440) are characteristic of iron oxide encapsulated in PMMA nanoparticles [4,17].…”

Section: Characterization Of Lauryl Gallate Loaded In Superparamagnet...mentioning

confidence: 99%

“…The very flexible nature of miniemulsion polymerization allows the incorporation of several compounds inside the polymeric nanoparticles as well as the surface modification to improve the efficacy of targeted drug delivery [15,16]. Folic acid (FA) has been used to modify the surface of polymeric nanoparticles increasing the efficiency of targeted drug delivery in cancer treatment [17][18][19][20] as the folate receptor is significantly overexpressed on the surface of different human cancer cells [16,18,19,21,22]. Folate receptor-mediated drug delivery is based on conjugation with FA, which promotes drug uptake by folate receptormediated endocytosis [21,23,24].…”

Section: Introductionmentioning

confidence: 99%

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Increased cellular uptake of lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles due to surface modification with folic acid

Feuser

Arévalo

Lima

et al. 2016

J Mater Sci: Mater Med

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Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles surface modified with folic acid were synthesized by miniemulsion polymerization in just one step. In vitro biocompatibility and cytotoxicity assays on L929 (murine fibroblast), human red blood, and HeLa (uterine colon cancer) cells were performed. The effect of folic acid at the nanoparticles surface was evaluated through cellular uptake assays in HeLa cells. Results showed that the presence of folic acid did not affect substantially the polymer particle size (~120 nm), the superparamagnetic behavior, the encapsulation efficiency of lauryl gallate (~87 %), the Zeta potential (~38 mV) of the polymeric nanoparticles or the release profile of lauryl gallate. The release profile of lauryl gallate from superparamagnetic poly(methyl methacrylate) nanoparticles presented an initial burst effect (0-1 h) followed by a slow and sustained release, indicating a biphasic release system. Lauryl gallate loaded in superparamagnetic poly(methyl methacrylate) nanoparticles with folic acid did not present cytotoxicity effects on L929 and human red blood cells. However, free lauryl gallate presented significant cytotoxic effects on L929 and human red blood cells at all tested concentrations. The presence of folic acid increased the cytotoxicity of lauryl gallate loaded in nanoparticles on HeLa cells due to a higher cellular uptake when HeLa cells were incubated at 37 °C. On the other hand, when the nanoparticles were incubated at low temperature (4 °C) cellular uptake was not observed, suggesting that the uptake occurred by folate receptor mediated energy-dependent endocytosis. Based on presented results our work suggests that this carrier system can be an excellent alternative in targeted drug delivery by folate receptor.

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