Functionalized fluorescent carbon nanostructures for targeted imaging of cancer cells: a review

Pirsaheb, Meghdad; Mohammadi, Somayeh; Salimi, Abdollah; Payandeh, Mehrdad

doi:10.1007/s00604-019-3338-4

Cited by 83 publications

(33 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carbon dots (CDs) have received extensive attention in recent years due to their stable optical properties [18], good biocompatibility [19], and low toxicity [20]. CDs have shown great potential in the study of bioimaging [21,22], photodynamic therapy [23], biosensing [24,25], drug carriers [26,27], and disease treatment [28]. In recent years, various CD-based nanoprobe strategies for intracellular pH sensing have emerged.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, various CD-based nanoprobe strategies for intracellular pH sensing have emerged. Most of the strategies can be divided into two types: One type is based on the increase or decrease of fluorescence intensity [21,29,30]. Wu et al reported nitrogen-doped graphene quantum dots as a quantitative pH sensor, and the increase of the fluorescence intensity was observed with the increase of the pH values [31].…”

Section: Introductionmentioning

confidence: 99%

“…Wu et al reported nitrogen-doped graphene quantum dots as a quantitative pH sensor, and the increase of the fluorescence intensity was observed with the increase of the pH values [31]. Chandra et al synthesized pH-sensitive CDs, the fluorescence intensity of which increased as the pH was changed from 7.4 to 5.0 [21]. Recently, Wang et al reported that the fluorescence intensity of CDs depends on the pH value in the range from 6.0 to 10.0 and showed excellent stability against the influence of metal ions and amino acids [32].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

et al. 2020

View full text Add to dashboard Cite

The monitoring of intracellular pH is of great importance for understanding intracellular trafficking and functions. It has various limitations for biosensing based on the fluorescence intensity or spectra study. In this research, pH-sensitive carbon dots (CDs) were employed for intracellular pH sensing with fluorescence lifetime imaging microscopy (FLIM) for the first time. FLIM is a highly sensitive method that is used to detect a microenvironment and it can overcome the limitations of biosensing methods based on fluorescence intensity. The different groups on the CDs surfaces changing with pH environments led to different fluorescence lifetime values. The CDs aqueous solution had a gradual change from 1.6 ns to 3.7 ns in the fluorescence lifetime with a pH range of 2.6–8.6. Similar fluorescence lifetime changes were found in pH buffer-treated living cells. The detection of lysosomes, cytoplasm, and nuclei in living cells was achieved by measuring the fluorescence lifetime of CDs. In particular, a phasor FLIM analysis was used to improve the pH imaging. Moreover, the effects of the coenzymes, amino acids, and proteins on the fluorescence lifetime of CDs were examined in order to mimic the complex microenvironment inside the cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

et al. 2020

View full text Add to dashboard Cite

show abstract

“…They can be obtained from various organic objects and degrade in a natural environment to CO 2 . Due to their strong photoluminescence, non-toxic nature, good chemical stability, and biocompatibility CDs have great potential in energy conversion and storage [1], water disinfection [2], food safety [3] analytical chemistry [4][5][6] for development of chemical-and bio-sensors [6][7][8], biotechnology and nanomedicine [9][10][11] in cell imaging and biolabeling [12][13][14][15], for drug release [16], theranostics [9,17], and photodynamic therapy [18], etc.…”

Section: Introductionmentioning

confidence: 99%

Benefit of porous silica nanoreactor in preparation of fluorescence carbon dots from citric acid

et al. 2020

View full text Add to dashboard Cite

A facile and robust synthesis of carbon dots (CDs) emitting blue-light in water without activation and stabilization has been developed by pyrolysis of citric acid (CA) adsorbed in silica gel (SiO 2 ) pores. Effect of the host pore size on luminescent properties of SiO 2 @CDs nanocomposite as well as water suspension of CDs has been studied. The synthesis conditions such as concentration of the precursor, duration of synthesis also have been investigated. It has been demonstrated that upon the thermal treatment of silica gels saturated with CA (60% of maximum loading) at 170°C for 5-600 min, luminescent CDs are shaped inside the nanoreactor pores. These SiO 2 @CDs emit photoluminescence centered at 450 nm. Silica-immobilized CDs can be separated from the source molecules and sideproducts by centrifugation, which allows avoiding the dialysis of the resulted mixture and so improve the scaling of the synthesis. The CDs can be easily released from SiO 2 @CDs by washing it with water. Water-eluted CDs demonstrate photoluminescence at 447 nm. The smaller pore size of the host and longer time of thermal treatment promote the formation of the CDs with better photoluminescent peak symmetry and higher quantum yield up to 10.1%.

show abstract

“…Coming to the applications in biomedicine, some studies have pointed out the anti-inflammatory properties of treated CNSs [14] although mechanisms behind this behavior has still not fully understood. More defined is the role of CNSs in fighting cancer diseases [15] as fluorescent markers for tumor tissue detection [16][17][18], sensors for early cancer detection [19][20][21], drug carriers [22,23], cancer phototherapy [24] and theranostics in general [25,26]. Recently, CNSs have been used to directly influence the biological activities of living matter.…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Carbon Nanomaterials for Biomedical Applications

Liu

Speranza

2019

View full text Add to dashboard Cite

Over the past decade, carbon nanostructures (CNSs) have been widely used in a variety of biomedical applications. Examples are the use of CNSs for drug and protein delivery or in tools to locally dispense nucleic acids to fight tumor affections. CNSs were successfully utilized in diagnostics and in noninvasive and highly sensitive imaging devices thanks to their optical properties in the near infrared region. However, biomedical applications require a complete biocompatibility to avoid adverse reactions of the immune system and CNSs potentials for biodegradability. Water is one of the main constituents of the living matter. Unfortunately, one of the disadvantages of CNSs is their poor solubility. Surface functionalization of CNSs is commonly utilized as an efficient solution to both tune the surface wettability of CNSs and impart biocompatible properties. Grafting functional groups onto the CNSs surface consists in bonding the desired chemical species on the carbon nanoparticles via wet or dry processes leading to the formation of a stable interaction. This latter may be of different nature as the van Der Waals, the electrostatic or the covalent, the π-π interaction, the hydrogen bond etc. depending on the process and on the functional molecule at play. Grafting is utilized for multiple purposes including bonding mimetic agents such as polyethylene glycol, drug/protein adsorption, attaching nanostructures to increase the CNSs opacity to selected wavelengths or provide magnetic properties. This makes the CNSs a very versatile tool for a broad selection of applications as medicinal biochips, new high-performance platforms for magnetic resonance (MR), photothermal therapy, molecular imaging, tissue engineering, and neuroscience. The scope of this work is to highlight up-to-date using of the functionalized carbon materials such as graphene, carbon fibers, carbon nanotubes, fullerene and nanodiamonds in biomedical applications.

show abstract

Functionalized fluorescent carbon nanostructures for targeted imaging of cancer cells: a review

Cited by 83 publications

References 72 publications

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Carbon Dots for Intracellular pH Sensing with Fluorescence Lifetime Imaging Microscopy

Benefit of porous silica nanoreactor in preparation of fluorescence carbon dots from citric acid

Functionalization of Carbon Nanomaterials for Biomedical Applications

Contact Info

Product

Resources

About