Electron microscopy cytochemistry and three-dimensional reconstruction of labeled structures in Trypanosoma cruzi

Differentiating Trypanosoma cruzi in a Host Mammalian Cell Imaged in Aqueous Liquid by Atmospheric Scanning Electron Microscopy

“…On the other hand, TEM has an advantage in investigating detailed subcellular structures, but acquired images are not very intuitive due to the two dimensionality of ultra-thin-sliced specimen. In recent years, 3D reconstruction of serial thin-section TEM images and FIB-SEM tomography overcame those disadvantages, yielding high resolution 3D visuals of intracellular T. cruzi ( 6 , 7 ). Few drawbacks of these techniques are that image acquisition and processing can be time-consuming and that original sample is unrecoverable due to sectioning or beam milling.…”