Manganese(II) adsorption and oxidation by whole cells and a membrane fraction of Pedomicrobium sp. ACM 3067

Larsen, Eloise; Sly, Lindsay I.; McEwan, Alastair G.

doi:10.1007/s002030050708

Cited by 83 publications

(70 citation statements)

References 49 publications

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“…SG-1 exosporium in the presence of pyrophosphate (196 µM) (Webb et al 2005), but much higher than that determined for other Mn(II)-oxidizing proteins from vegetative cells: 7 µM with Leptothrix discophora SS-1 (Adams and Ghiorse 1987), 10 µM with Pseudomonas putida GB-1 (Okazaki et al 1997), 26 µM with Pedomicrobium sp. ACM 3067 (Larsen et al 1999). The results of inhibition with azide, cyanide, and o-phenanthroline are relatively consistent with what has previously been reported, but do not conclusively suggest the involvement of an MCO because these compounds may also interact with Mn.…”

Section: Discussionsupporting

confidence: 58%

“…SD21. This stimulation has been shown in other Mn(II)-oxidizing bacteria (van Waasbergen et al 1996;Larsen et al 1999;Brouwers et al 2000a;Zhang et al 2002). With growing whole cells of Erythrobacter sp.…”

Section: Optimization Of the Mn(ii) Oxidase Assay And Identification mentioning

confidence: 54%

“…The absorbance spectra of the partially purified activity does not show characteristics consistent with an MCO, and copper does not affect Mn (II) oxidation by Erythrobacter sp. SD21 in vivo or in vitro as has been shown in other Mn (II)-oxidizing microorganisms (van Waasbergen et al 1996;Larsen et al 1999;Brouwers et al 2000a;Zhang et al 2002). In addition, in contrast to what has been observed with other Mn (II)-oxidizing protein preparations (Webb et al 2004), Mn(III) instead of Mn(IV) seems to be the product of Mn(II) oxidation; thus, a different catalytic mechanism may be employed.…”

Section: Discussionmentioning

confidence: 92%

“…Studies on Mn(II) oxidation in α-proteobacteria have been limited, yet the effect of copper on Mn(II) oxidation in whole cells (Larsen et al 1999), the non Mn(II) oxidizing phenotype of a MCO mutant (Ridge et al 2007), and the effect of copper chelators on Mn(II) oxidation in acrylamide gels (Francis et al 2001) suggest copper may play an important role in Mn(II) oxidation in this phylogenetic group. The marine α-proteobacterium Erythrobacter sp.…”

Section: Introductionmentioning

confidence: 99%

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In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation

Johnson

Tebo

2007

Arch Microbiol

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Manganese(II)-oxidizing bacteria play an integral role in the cycling of Mn as well as other metals and organics. Prior work with Mn(II)-oxidizing bacteria suggested that Mn(II) oxidation involves a multicopper oxidase, but whether this enzyme directly catalyzes Mn(II) oxidation is unknown. For a clearer understanding of Mn(II) oxidation, we have undertaken biochemical studies in the model marine α-proteobacterium, Erythrobacter sp. strain SD21. The optimum pH for Mn(II)-oxidizing activity was 8.0 with a specific activity of 2.5 nmol × min −1 × mg −1 and a K m = 204 µM. The activity was soluble suggesting a cytoplasmic or periplasmic protein. Mn(III) was an intermediate in the oxidation of Mn(II) and likely the primary product of enzymatic oxidation. The activity was stimulated by pyrroloquinoline quinone (PQQ), NAD + , and calcium but not by copper. In addition, PQQ rescued Pseudomonas putida MnB1 non Mn(II)-oxidizing mutants with insertions in the anthranilate synthase gene. The substrate and product of anthranilate synthase are intermediates in various quinone biosyntheses. Partially purified Mn(II) oxidase was enriched in quinones and had a UV/VIS absorption spectrum similar to a known quinone requiring enzyme but not to multicopper oxidases. These studies suggest that quinones may play an integral role in bacterial Mn(II) oxidation.

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Section: Discussionsupporting

confidence: 58%

Section: Optimization Of the Mn(ii) Oxidase Assay And Identification mentioning

confidence: 54%

Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation

Johnson

Tebo

2007

Arch Microbiol

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“…Although there has been some prior remetal residence time and phase distribution in most aquatic search on the mechanisms of enzymatic catalysis of Mn(II) systems. Particulate organic material and Fe/Mn oxides have oxidation (Larsen et al, 1999;Tebo et al, 1997), no compre been identified as prominent solid phases controlling transition hensive rate equation has been proposed for microbially medi metal adsorption (Vuceta and Morgan, 1978;Luoma and ated Mn(II) oxidation kinetics. Bryan, 1981;Tessier et al, 1996;Nelson et al, 1999a;Nelson et al, 1999b;Dong et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Mn(II) oxidation by Leptothrix discophora SS1

Zhang

Lion

Nelson

et al. 2002

Geochimica et Cosmochimica Acta

103

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Abstract-The kinetics of Mn(II) oxidation by the bacterium Leptothrix discophora SS1 was investigated in this research. Cells were grown in a minimal mineral salts medium in which chemical speciation was well defined. Mn(II) oxidation was observed in a bioreactor under controlled conditions with pH, O 2 , and temperature regulation. Mn(II) oxidation experiments were performed at cell concentrations between 24 mg/L and 35 mg/L, over a pH range from 6 to 8.5, between temperatures of 10°C and 40°C, over a dissolved oxygen range of 0 to 8.05 mg/L, and with L. discophora SS1 cells that were grown in the presence of Cu concentrations ranging from zero to 0.1 �M. Mn(II) oxidation rates were determined when the cultures grew to stationary phase and were found to be directly proportional to O 2 and cell concentrations over the ranges investigated. The optimum pH for Mn(II) oxidation was approximately 7.5, and the optimum temperature was 30°C. A Cu level as low as 0.02 �M was found to inhibit the growth rate and yield of L. discophora SS1 observed in shake flasks, while Cu levels between 0.02 and 0.1 �M stimulated the Mn(II) oxidation rate observed in bioreactors. An overall rate law for Mn(II) oxidation by L. discophora as a function of pH, temperature, dissolved oxygen concentration (D.O.), and Cu concentration is proposed. At circumneutral pH, the rate of biologically mediated Mn(II) oxidation is likely to exceed homogeneous abiotic Mn(II) oxidation at relatively low (��g/L) concentrations of Mn oxidizing bacteria.

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Seasonal impacts on biofiltration acclimation dynamics and performance

et al. 2021

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The City of Denton, Texas, upgraded the Lake Lewisville Water Treatment Plant (WTP) to add ozone and convert the existing chlorinated anthracite/sand filters to granular activated carbon (GAC) biofilters. Biofilter contaminant removal, operational performance, and biomass were monitored following startup on two filters, one brought online in winter and one brought online in summer, to understand seasonal impacts. In addition, the city also monitored anthracite biofilters following a winter shutdown at another WTP. Key conclusions include (1) acclimation (i.e., the time required to achieve steady‐state biomass, total organic carbon [TOC] removal, or total manganese removal, considered individually) was two to three times longer for filters brought online in the winter versus the summer, (2) no acclimation period was observed for turbidity or total iron removal, and (3) the relationship between TOC removal and water temperature was more significant for the WTP with anthracite than GAC biofilters.

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Manganese(II) adsorption and oxidation by whole cells and a membrane fraction of Pedomicrobium sp. ACM 3067

Cited by 83 publications

References 49 publications

In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation

In vitro studies indicate a quinone is involved in bacterial Mn(II) oxidation

Kinetics of Mn(II) oxidation by Leptothrix discophora SS1

Seasonal impacts on biofiltration acclimation dynamics and performance

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