Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat

Baker, Louise; Grewal, Surbhi; Yang, Caiyun; Hubbart-Edwards, Stella; Scholefield, Duncan; Ashling, Stephen; Burridge, Amanda J.; Przewieslik-Allen, Alexandra M.; Wilkinson, Paul; King, Ian P.; King, Julie

doi:10.1007/s00122-020-03591-3

Cited by 27 publications

(13 citation statements)

References 60 publications

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“…Discovery of the Ph1 locus led to the possibility of recombining non-homologous genomes with those of wheat. The same approach is now being followed in many other cereals [143,[185][186][187] and has been extended to include a wider range of CWR in wheat [188]. Genomic analyses can reveal the translocations and rearrangements that have been introduced, allowing for more structured and efficient screening of the huge array of novel recombinations that can be generated.…”

Section: Status Of Pgr Use For Wheat Improvementmentioning

confidence: 99%

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Sharma¹,

Schulthess

Bassi³

et al. 2021

Biology

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Wheat (Triticum sp.) is one of the world’s most important crops, and constantly increasing its productivity is crucial to the livelihoods of millions of people. However, more than a century of intensive breeding and selection processes have eroded genetic diversity in the elite genepool, making new genetic gains difficult. Therefore, the need to introduce novel genetic diversity into modern wheat has become increasingly important. This review provides an overview of the plant genetic resources (PGR) available for wheat. We describe the most important taxonomic and phylogenetic relationships of these PGR to guide their use in wheat breeding. In addition, we present the status of the use of some of these resources in wheat breeding programs. We propose several introgression schemes that allow the transfer of qualitative and quantitative alleles from PGR into elite germplasm. With this in mind, we propose the use of a stage-gate approach to align the pre-breeding with main breeding programs to meet the needs of breeders, farmers, and end-users. Overall, this review provides a clear starting point to guide the introgression of useful alleles over the next decade.

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Section: Status Of Pgr Use For Wheat Improvementmentioning

confidence: 99%

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Sharma¹,

Schulthess

Bassi³

et al. 2021

Biology

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“…The use of the new technologies at the Nottingham BBSRC Wheat Research Centre (WRC) has allowed the transfer of hundreds of introgressions from a variety of wild relatives into wheat, e.g., Amblyopyrum muticum ( King et al, 2017 ), Thinopyrum bessarabicum ( Grewal et al, 2018b ), Ae. speltoides ( King et al, 2018 ), T. urartu ( Grewal et al, 2018a ), Triticum timopheevii ( Devi et al, 2019 ), Thinopyrum elongatum ( Baker et al, 2020 ), and Aegilops caudata ( Grewal et al, 2020b ). However, without identifying what genetic variation is carried by the introgressions, the lines produced will remain quite simply as ‘seeds in a packet’ of unknown agronomic potential.…”

Section: Introductionmentioning

confidence: 99%

Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers

et al. 2021

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Wheat is one of the most important food and protein sources in the world and although, in recent years wheat breeders have achieved yield gains, they are not sufficient to meet the demands of an ever-growing population. Development of high yielding wheat varieties, resilient to abiotic and biotic stress resulting from climate change, has been limited by wheat’s narrow genetic base. In contrast to wheat, the wild relatives of wheat provide a vast reservoir of genetic variation for most, if not all, agronomic traits. Previous studies by the authors have shown the transfer of genetic variation from T. urartu into bread wheat. However, before the introgression lines can be exploited for trait analysis, they are required to have stable transmission of the introgressions to the next generation. In this work, we describe the generation of 86 doubled haploid (DH) wheat-T. urartu introgression lines that carry homozygous introgressions which are stably inherited. The DH lines were characterised using the Axiom® Wheat Relative Genotyping Array and 151 KASP markers to identify 65 unique T. urartu introgressions in a bread wheat background. DH production has helped accelerate the breeding process and facilitated the early release of homozygous wheat-T. urartu introgression lines. Together with the KASP markers, this valuable resource could greatly advance identification of beneficial alleles that can be used in wheat improvement.

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“…Genotyping of introgression lines is more efficient when using wild-relative genomespecific SNPs. The Axiom ® Wheat-Relative Genotyping SNP Array was developed (King et al 2017) and used to detect introgressions from various wild species in a wheat background (Grewal et al 2018a;Grewal et al 2018b;King et al 2018;Cseh et al 2019a;Devi et al 2019;Baker et al 2020). Even though these genotyping arrays can be ultrahigh-throughput and efficient, these SNPs cannot distinguish between homozygous and heterozygous individuals which limits their widespread use in crop breeding.…”

Section: Introductionmentioning

confidence: 99%

A novel approach to develop wheat chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in doubled haploid introgression lines

Grewal

Coombes

Joynson

et al. 2021

Preprint

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Many wild relative species are being used in pre-breeding programmes to increase the genetic diversity of wheat. Genotyping tools such as single nucleotide polymorphism (SNP)-based arrays and molecular markers have been widely used to characterise wheat-wild relative introgression lines. However, due to the polyploid nature of the recipient wheat genome, it is difficult to develop SNP-based KASP markers that are codominant to track the introgressions from the wild species. Previous attempts to develop KASP markers have involved both exome- and PCR-amplicon-based sequencing of the wild species. But chromosome-specific KASPs assays have been hindered by homoeologous SNPs within the wheat genome. This study involved whole genome sequencing of the diploid wheat wild relative Amblyopyrum muticum and development of a SNP discovery pipeline that generated ~38,000 SNPs in single-copy wheat genome sequences. New assays were designed to increase the density of Am. muticum polymorphic KASP markers. With a goal of one marker per 60 Mbp, 335 new KASP assays were validated as functional. Together with assays validated in previous studies, 498 well distributed chromosome-specific markers were used to recharacterize previously genotyped wheat-Am. muticum doubled haploid (DH) introgression lines. The chromosome specific nature of the KASP markers allowed clarification of which wheat chromosomes were involved with recombination events or substituted with Am. muticum chromosomes and the higher density of markers allowed detection of new small introgressions in these DH lines.

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Exploiting the genome of Thinopyrum elongatum to expand the gene pool of hexaploid wheat

Cited by 27 publications

References 60 publications

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Introducing Beneficial Alleles from Plant Genetic Resources into the Wheat Germplasm

Generation of Doubled Haploid Wheat-Triticum urartu Introgression Lines and Their Characterisation Using Chromosome-Specific KASP Markers

A novel approach to develop wheat chromosome-specific KASP markers for detecting Amblyopyrum muticum segments in doubled haploid introgression lines

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