Development of mlo-based resistance in tetraploid wheat against wheat powdery mildew

Ingvardsen, Christina; Massange-Sánchez, Julio A.; Borum, Finn; Uauy, Cristóbal; Gregersen, Per L.

doi:10.1007/s00122-019-03402-4

Cited by 17 publications

(11 citation statements)

References 32 publications

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“…This set of plants is, therefore, well suited to study the effect of Tamlo mutations on the outcome of interactions with other pathogens and the occurrence and strength of putative pleiotropic phenotypes. A similar panel of TILLING-based mlo mutants covering a broad range of powdery mildew susceptibility/resistance was recently described for tetraploid durum wheat (T. turgidum; [19]).…”

Section: Discussionmentioning

confidence: 98%

“…By contrast, the non-transgenic approach, achieved via targeted induced local lesions in genomes (TILLING) technology resulting in missense mutations in the TaMlo homoeologs, yielded mutants with residual activity and accordingly partial Bgt resistance [18]. Recently, TILLING-derived mlo mutants were also reported in tetraploid durum wheat (Triticum turgidum [19]).…”

Section: Introductionmentioning

confidence: 99%

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Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum

Grüner

Esser

Acevedo‐Garcia

et al. 2020

Genes

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Barley mlo mutants are well known for their profound resistance against powdery mildew disease. Recently, mlo mutant plants were generated in hexaploid bread wheat (Triticum aestivum) with the help of transgenic (transcription-activator-like nuclease, TALEN) and non-transgenic (targeted induced local lesions in genomes, TILLING) biotechnological approaches. While full-gene knockouts in the three wheat Mlo (TaMlo) homoeologs, created via TALEN, confer full resistance to the wheat powdery mildew pathogen (Blumeria graminis f.sp. tritici), the currently available TILLING-derived Tamlo missense mutants provide only partial protection against powdery mildew attack. Here, we studied the infection phenotypes of TALEN- and TILLING-derived Tamlo plants to the two hemibiotrophic pathogens Zymoseptoria tritici, causing Septoria leaf blotch in wheat, and Magnaporthe oryzae pv. Triticum (MoT), the causal agent of wheat blast disease. While Tamlo plants showed unaltered outcomes upon challenge with Z. tritici, we found evidence for allele-specific levels of enhanced susceptibility to MoT, with stronger powdery mildew resistance correlated with more invasive growth by the blast pathogen. Surprisingly, unlike barley mlo mutants, young wheat mlo mutant plants do not show undesired pleiotropic phenotypes such as spontaneous callose deposits in leaf mesophyll cells or signs of early leaf senescence. In conclusion, our study provides evidence for allele-specific levels of enhanced susceptibility of Tamlo plants to the hemibiotrophic wheat pathogen MoT.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum

Grüner

Esser

Acevedo‐Garcia

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

“…This set of plants is therefore well suited to study the effect of Tamlo mutations on the outcome of interactions with other pathogens and the occurrence and strength of putative pleiotropic phenotypes. A similar panel of TILLING-based mlo mutants covering a broad range of powdery mildew susceptibility/resistance was recently described for tetraploid durum wheat (T. turgidum; (Ingvardsen et al, 2019)).…”

Section: Discussionmentioning

confidence: 98%

“…By contrast, the non-transgenic approach, achieved via targeted induced local lesions in genomes (TILLING) technology resulting in missense mutations in the TaMlo homoeologs, yielded mutants with residual activity and accordingly partial Bgt resistance (Acevedo-Garcia et al, 2017b). Recently, TILLING-derived mlo mutants were also reported in tetraploid durum wheat (Triticum turgidum (Ingvardsen et al, 2019)).…”

Section: Introductionmentioning

confidence: 99%

Wheat mlo Mutants Show Allele-Specific Levels of Enhanced Susceptibility to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum

Grüner¹,

Esser²,

Acevedo‐Garcia³

et al. 2020

Preprint

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Barley mlo mutants are well known for their profound resistance against powdery mildew disease. Recently, mlo mutant plants were generated in hexaploid bread wheat (Triticum aestivum) with the help of transgenic (transcription-activator-like nuclease, TALEN) and non-transgenic (targeted induced local lesions in genomes, TILLING) biotechnological approaches. While full gene knockouts in the three wheat Mlo (TaMlo) homoeologs, created via TALEN, confer full resistance to the wheat powdery mildew pathogen (Blumeria graminis f.sp. tritici), the currently available TILLING-derived Tamlo missense mutants provide only partial protection against powdery mildew attack. Here we studied the infection phenotypes of TALEN- and TILLING-derived Tamlo plants to the two hemibiotrophic pathogens Zymoseptoria tritici, causing Septoria leaf blotch in wheat, and Magnaporthe oryzae pv. Triticum (MoT), the causal agent of wheat blast disease. While Tamlo plants showed unaltered outcomes upon challenge with Z. tritici, we found allele-specific levels of enhanced susceptibility to MoT, with stronger powdery mildew resistance correlated with more invasive growth by the blast pathogen. Surprisingly, unlike barley mlo mutants, young wheat mlo mutant plants do not show undesired pleiotropic phenotypes such as spontaneous callose deposits in leaf mesophyll cells or signs of early leaf senescence. In conclusion, our study provides evidence for allele-specific levels of enhanced susceptibility of Tamlo plants to the hemibiotrophic wheat pathogen MoT.

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“…Common wheat (Triticum aestivum L.) is one of the three major grain crops worldwide, and its high and stable yield plays an important role in food security. However, various diseases, including powdery mildew, rusts, and fusarium head blight, can have devastating impacts on yield (Huang and Pang, 2017;Ingvardsen et al, 2019;Li et al, 2019b). Powdery mildew caused by Blumeria graminis f. sp.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection

Jia

Liu

et al. 2020

Front. Genet.

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Development of mlo-based resistance in tetraploid wheat against wheat powdery mildew

Cited by 17 publications

References 32 publications

Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum

Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum

Wheat <em>mlo</em> Mutants Show Allele-Specific Levels of Enhanced Susceptibility to the Hemibiotrophic Fungal Pathogen <em>Magnaporthe oryzae</em> pv. <em>Triticum</em>

Characterization of the Powdery Mildew Resistance Gene in the Elite Wheat Cultivar Jimai 23 and Its Application in Marker-Assisted Selection

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