Fine mapping of Aegilops peregrina co-segregating leaf and stripe rust resistance genes to distal-most end of 5DS

Narang, Deepika; Kaur, Satinder; Steuernagel, Burkhard; Ghosh, Sreya; Dhillon, Roopan; Bansal, Mitaly; Uauy, Cristóbal; Wulff, Brande B. H.; Chhuneja, Parveen

doi:10.1007/s00122-019-03293-5

Cited by 8 publications

(3 citation statements)

References 49 publications

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“…Roughly half of these genes are from wild relatives of wheat, while the remainder are from cultivated wheat ( Marais et al, 2005 ; Naik et al, 2015 ; Rani et al, 2020 ). Wild relatives of wheat provide a huge gene pool of agronomy utility, including genes for rust resistance ( Narang et al, 2019 ). The D genome donor of wheat, Aegilops tauschii , has been a rich source of resistance genes ( Gill et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping of the Leaf Rust Resistance Gene Lr65 in Spelt Wheat ‘Altgold’

et al. 2021

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Wheat leaf rust (also known as brown rust), caused by the fungal pathogen Puccinia triticina Erikss. (Pt), is one by far the most troublesome wheat disease worldwide. The exploitation of resistance genes has long been considered as the most effective and sustainable method to control leaf rust in wheat production. Previously the leaf rust resistance gene Lr65 has been mapped to the distal end of chromosome arm 2AS linked to molecular marker Xbarc212. In this study, Lr65 was delimited to a 0.8 cM interval between flanking markers Alt-64 and AltID-11, by employing two larger segregating populations obtained from crosses of the resistant parent Altgold Rotkorn (ARK) with the susceptible parents Xuezao and Chinese Spring (CS), respectively. 24 individuals from 622 F2 plants of crosses between ARK and CS were obtained that showed the recombination between Lr65 gene and the flanking markers Alt-64 and AltID-11. With the aid of the CS reference genome sequence (IWGSC RefSeq v1.0), one SSR marker was developed between the interval matched to the Lr65-flanking marker and a high-resolution genetic linkage map was constructed. The Lr65 was finally located to a region corresponding to 60.11 Kb of the CS reference genome. The high-resolution genetic linkage map founded a solid foundation for the map-based cloning of Lr65 and the co-segregating marker will facilitate the marker-assisted selection (MAS) of the target gene.

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Section: Introductionmentioning

confidence: 99%

Fine Mapping of the Leaf Rust Resistance Gene Lr65 in Spelt Wheat ‘Altgold’

et al. 2021

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“…Compared with other kinds of markers, kompetitive allele-specific PCR (KASP) assays accelerate the conversion of DNA variations into available gene-linked markers. Taking advantage of such whole genomic sequences, Wu et al (2018b) mapped wheat yellow rust resistance gene Yr26 on a 0.003-cM interval on chromosome 1B near the centromere, Narang et al (2019) defined wheat leaf rust resistance gene LrP and yellow rust resistance gene YrP on a 15.71 Mb region on 5DS in the CS RefSeq v1.0 genome assembly, and Wu et al (2019) localized the Pm52 locus within a 5.6 Mb interval on the long arm of chromosome 2B (2BL).…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping of the Wheat Leaf Rust Resistance Gene LrLC10 (Lr13) and Validation of Its Co-segregation Markers

Qiu

Wang

et al. 2020

Front. Plant Sci.

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Wheat leaf rust, caused by the fungus Puccinia triticina Eriks. (Pt), is a destructive disease found throughout common wheat production areas worldwide. At its adult stage, wheat cultivar Liaochun10 is resistant to leaf rust and the gene for that resistance has been mapped on chromosome 2BS. It was designated LrLC10 and is the same gene as cataloged gene Lr13 by pedigree analysis and allelism test. We fine-mapped it using recessive class analysis (RCA) of the homozygous susceptible F 2 plants derived from crosses using Liaochun10 as the resistant, male parent. Taking advantage of the re-sequencing data of Liaochun10 and its counterpart susceptible parent, we converted nucleotide polymorphisms in the LrLC10 interval between the resistant and susceptible parents into molecular markers to saturate the LrLC10 genetic linkage map. Four indel markers were added in the 1.65 cM map of LrLC10 flanked by markers CAUT163 and Lseq22. Thirty-two recombinants were identified by those two markers from the 984 F 2 homozygous susceptible plants and were further genotyped with additional ten markers. LrLC10 was finally placed in a 314.3 kb region on the Chinese Spring reference sequence (RefSeq v1.0) that contains three high confidence genes: TraesCS2B01G182800, TraesCS2B01G182900, and TraesCS2B01G183000. Sequence analysis showed several variations in TraesCS2B01G182800 and TraesCS2B01G183000 between resistant and susceptible parents. One KASP marker and an indel marker were designed based on the differences in those two genes, respectively, and were validated to be diagnostic co-segregating markers for LrLC10. Our results both improve markerassisted selection and help with the map-based cloning of LrLC10.

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“…Recently, a QTL genetic marker was co-localized for yellow rust on chromosome 6BL in wheat (Zeng et al 2019). Similarly, the leaf and yellow rust genes have been fine mapped to 5D chromosome of Aegilops peregrine, a wild relative of common wheat (Narang et al 2019). MAS is therefore, a helpful tool to identify cultivars harboring alleles for the traits of interest at specific loci, and develop varieties of improved yield and high rust resistance in wheat.…”

Section: Introductionmentioning

confidence: 99%

DUAL OPTIMIZATION OF MEDIUM COMPONENTS USING WEIGHTED STANDARDIZATION FOR Antibacterial SPECTRUM AND CELL YIELD OF Lactobacillus plantarum SK1305 Isolated from Pickled Pepper

2020

THE JAPS

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Fine mapping of Aegilops peregrina co-segregating leaf and stripe rust resistance genes to distal-most end of 5DS

Cited by 8 publications

References 49 publications

Fine Mapping of the Leaf Rust Resistance Gene Lr65 in Spelt Wheat ‘Altgold’

Fine Mapping of the Leaf Rust Resistance Gene Lr65 in Spelt Wheat ‘Altgold’

Fine Mapping of the Wheat Leaf Rust Resistance Gene LrLC10 (Lr13) and Validation of Its Co-segregation Markers

DUAL OPTIMIZATION OF MEDIUM COMPONENTS USING WEIGHTED STANDARDIZATION FOR Antibacterial SPECTRUM AND CELL YIELD OF Lactobacillus plantarum SK1305 Isolated from Pickled Pepper

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