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Cited by 859 publications
(600 citation statements)
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“…The total DNA was extracted from leaf samples using CTAB method [29,32]. The details of primers used for amplification of different DNA genomic components of BBTV were given in the Table 1.…”
Section: Methodsmentioning
confidence: 99%
“…The total DNA was extracted from leaf samples using CTAB method [29,32]. The details of primers used for amplification of different DNA genomic components of BBTV were given in the Table 1.…”
Section: Methodsmentioning
confidence: 99%
“…Para extração do DNA genômico, foram utilizadas folhas jovens de alface-d'água de cada acesso, seguindo a metodologia descrita por Lodhi et al (1994). Na análise das diversidades genéticas entre as plantas foi empregada a téc-nica RAPD, do protocolo original de Williams et al (1990), utilizando 37 primers de seqüên-cias arbitrárias de 10 bases, provenientes da coleç ão da University of Brit ish Colum bia, Nucleic Acid Protein Service Unit (Canadá) e os produzidos pela Operon Technologies.…”
Section: Methodsunclassified
“…This may be due to the presence of inhibitors that are present in wine. Plant materials, such as polysaccharides, plant lipids and polyphenols are known to inhibit PCR reactions, which ultimately will also influence the outcome of the DGGE detection limit results (Lodhi et al, 1994). The plant material and inhibitory substances that were extracted during DNA isolation could also have had an influence on the PCR amplification of the DNA template, and could cause a decrease in the sensitivity of this detection method (Prakitchaiwattana et al, 2004).…”
Section: Pediococcus Pentosaceusmentioning
confidence: 99%