Rapid method for distinction of gram-negative from gram-positive bacteria

Gregersen, Troels

doi:10.1007/bf00498806

Cited by 846 publications

(483 citation statements)

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“…The purity of the isolate was checked by microscopy,by substituting thiosulfate with yeast extract (1 g/L; absence of growth) and sequencing its 16 S rRNA gene. Detection of Gramnegative type of the cell wall was performed by rapid KOH string test (Gregersen 1978). The isolate was designated ALCO 1.…”

Section: Methodsmentioning

confidence: 99%

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

et al. 2008

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Section: Methodsmentioning

confidence: 99%

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

et al. 2008

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“…The KOH test (Gregersen, 1978) was used to test the Gram reaction. Anaerobic growth was assessed on TSB/10 agar (both with and without KNO 3 ) incubation in airtight jars containing an AnaeroPack (Oxoid).…”

mentioning

confidence: 99%

Kineococcus xinjiangensis sp. nov., isolated from desert sand

Liu¹,

Peng²,

Wang³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A brown-orange-pigmented, non-spore-forming, coccus-shaped actinomycete, designated S2-20 T , was isolated from desert sand from Xinjiang Province in China. The isolate stains Grampositive, is motile and produces a brownish diffusible pigment. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2-20 T was phylogenetically affiliated to the genus Kineococcus, and the sequence similarity to the type strains of Kineococcus species was less than 96 %, making it clear that strain S2-20 T represents a species that is separate from recognized Kineococcus species. Its major fatty acid was anteiso-C 15 : 0 . The major menaquinone was MK-9(H 2 ). Whole-cell hydrolysates of strain S2-20 T contained meso-diaminopimelic acid, arabinose and galactose. The DNA G+C content was 77.8 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain S2-20 T should be classified within a novel species of the genus Kineococcus, for which the name Kineococcus xinjiangensis sp. nov. is proposed. The type strain is S2-20 T (5CCTCC AB 207179 T 5KCTC 19474 T ).Various bacterial species have the capacity to survive under conditions that are commonly considered extreme, such as cold, hot, saline and dry environments. Because of our interest in radiation-resistant bacteria and the correlation that has been made between desiccation and radiation resistance (Mattimore & Battista, 1996), we have studied bacteria that survive in the desert. Desiccation-and radiation-resistant bacteria have been observed in several genera, including Deinococcus, Rubrobacter, Hymenobacter, Methylobacterium, Kocuria and Kineococcus (Rainey et al., 2005; Phillips et al., 2002). The genus Kineococcus was first created by Yokota et al. (1993) with the species Kineococcus aurantiacus, based on a single strain isolated from soil. Two more species have been added to the genus in recent years; Kineococcus radiotolerans and Kineococcus marinus were reported in 2002 and 2006, respectively. In the present study, a strain with diffusible pigment production was characterized as a novel member of the genus Kineococcus.Strain S2-20 T was isolated from a sand sample from a desert in Xinjiang Province using tenfold-diluted tryptic soy broth (TSB/10; Difco) agar. Colonies were brownorange, circular, convex with entire edges and produced a brownish diffusible pigment but not clusters after 7 days of incubation on TSB/10 agar at 30 u C. Cell morphology was examined by light microscopy (Olympus) and by electron microscopy (Hitachi) using methods published previously (Glauert, 1991;Bozzola & Russell, 1999). Motility was observed on motility agar (TSB/10 with 0.5 % agar). The isolate could also grow on tenfold-diluted marine agar 2216 (MA/10; Difco) but not on PTYG medium (Phillips et al., 2002).Genomic DNA for amplification of the 16S rRNA gene was extracted as described by Earl et al. (2002). The 16S rRNA gene was amplified by PCR with bacterial universal primers 27F and 1492R (Lane, 1991) and the PCR products were sequenced by Invi...

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“…Kertesz, personal communication). The Gram-reaction was assayed in the KOH test [12]. Oxidase tests were carried out following standard methods [13].…”

Section: Microbiological Methodsmentioning

confidence: 99%

Bacterial cleavage of nitrogen to sulfone bonds in sulfamide and 1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide: formation of 2-nitrobenzamide by Gordonia sp.

Rein

1999

FEMS Microbiology Letters

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Rapid method for distinction of gram-negative from gram-positive bacteria

Cited by 846 publications

References 2 publications

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes

Kineococcus xinjiangensis sp. nov., isolated from desert sand

Bacterial cleavage of nitrogen to sulfone bonds in sulfamide and 1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide: formation of 2-nitrobenzamide by Gordonia sp.

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