Reduction of dichromate by Thiobacillus ferrooxidans

Sisti, Federico; Allegretti, Patricia Ercilia; Donati, Edgardo R.

doi:10.1007/bf00129358

Cited by 19 publications

(4 citation statements)

References 12 publications

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“…The reduction of Cr(VI) has been reported for a number of bacterial genera, including Pseudomonas (Bopp and Ehrlich 1988;Gopalan and Veeramani 1994;Bhide et al 1996;Turick and Apel 1996), Bacillus (Campos et al 1995), Enterobacter (Wang et al 1989;Rege et al 1997), Thiobacillus (Sisti et al 1996) and sulphate-reducing bacteria (Fude et al 1994). In some cases, the reduction of Cr(VI) is indirectly mediated by the production of reducing agents as metabolites; for example, Thiobacillus ferrooxidans generates sulphite and thiosulphate which can reduce Cr(VI) at pH 1·7 (Sisti et al 1996), and sulphate-reducing bacteria reduce Cr(VI) indirectly via the production of sulphide (Fude et al 1994). In other cases, the bacteria utilize sugars (e.g.…”

Section: Discussionmentioning

confidence: 97%

Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1

Myers¹,

Carstens²,

Antholine

et al. 2001

Journal of Applied Microbiology

230

157

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D J . M. MY E RS . 2000. Shewanella putrefaciens MR-1 can reduce a diverse array of compounds under anaerobic conditions, including manganese and iron oxides, fumarate, nitrate, and many other compounds. These reductive processes are apparently linked to a complex electron transport system. Chromium (Cr) is a toxic and mutagenic metal and bacteria could potentially be utilized to immobilize Cr by reducing the soluble and bioavailable state, Cr(VI), to the insoluble and less bioavailable state, Cr(III). Formate-dependent Cr(VI) reductase activity was detected in anaerobically grown cells of S. putrefaciens MR-1, with highest specific activity in the cytoplasmic membrane. Both formate and NADH served as electron donors for Cr(VI) reductase, whereas L-lactate or NADPH did not support any activity. The addition of 10 mmol l −1 FMN markedly stimulated formate-dependent Cr(VI) reductase, and the activity was almost completely inhibited by diphenyliodonium chloride, an inhibitor of flavoproteins. Cr(VI) reductase activity was also inhibited by pchloromercuriphenylsulphonate, azide, 2-heptyl-4-hydroxyquinolone-N-oxide, and antimycin A, suggesting involvement of a multi-component electron transport chain which could include cytochromes and quinones. Cr(V) was detected by electron paramagnetic resonance (EPR) spectroscopy, suggesting a one-electron reduction as the first step.

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Section: Discussionmentioning

confidence: 97%

Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1

Myers¹,

Carstens²,

Antholine

et al. 2001

Journal of Applied Microbiology

230

157

View full text Add to dashboard Cite

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“…Cr(VI) species can be readily reduced to Cr(III) in the presence of naturally occurring organic matter [5,6,34], ferrous [Fe (II)] iron [11,19,31] and sulfide [31], these constituents act as reducing agent in aquifer sediments [4]. Cr(VI) can also be reduced by Thiobacillus ferrooxidans [28,33], by using trimanganese tetroxide, and by manganese (III) oxide [30].…”

Section: Introductionmentioning

confidence: 99%

Impact assessment of chromite mining on groundwater through simulation modeling study in Sukinda chromite mining area, Orissa, India

Dhakate

Singh

Hodlur

2008

Journal of Hazardous Materials

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“…Generally, biotechnology is a powerful and versatile alternative to chemical and physical methods for resolving many problems of environmental pollution because of low demand of energy and materials, and low generation of waste and emissions. Recently, biological chromium (VI) reduction has been developed with some advantages due to the lower costs and the significant smaller quantities of the produced sludge (Sisti et al 1996;Ganguli and Tripathi 1999;Konovalova et al 2003;Liu et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Bacterial removal of chromium (VI) and (III) in a continuous system

et al. 2006

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The capacity of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans to reduce different concentrations of hexavalent chromium in shake flask cultures has been investigated. A. ferrooxidans reduces 100% of chromium (VI) at concentrations of 1, 2.5 and 5 ppm, but in the presence of 10 ppm only 42.9% of chromium (VI) was reduced after 11 days of incubation. A. thiooxidans showed a lower capacity to reduce this ion and total reduction of chromium (VI) was only obtained for concentrations of 1 and 2.5 ppm, whereas 64.7% and 30.5% was reached for 5 and 10 ppm, respectively, after 11 days. A continuous flow mode system was subsequently investigated, in which A. thiooxidans was immobilized on elemental sulphur and the acidic medium obtained was employed to solubilize chromium (III) and to reduce chromium (VI) present in a real electroplating waste [30% of chromium (III) and 0.1% of chromium (VI)]. The system enabled the reduction of 92.7% of hexavalent chromium and represents a promising way to treat this type of waste in the industry.

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Reduction of dichromate by Thiobacillus ferrooxidans

Cited by 19 publications

References 12 publications

Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1

Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR-1

Impact assessment of chromite mining on groundwater through simulation modeling study in Sukinda chromite mining area, Orissa, India

Bacterial removal of chromium (VI) and (III) in a continuous system

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