Advancement of Genetic Engineering in Rhamnolipid(s) Production

Kumar, Rajesh; Das, Amar Jyoti

doi:10.1007/978-981-13-1289-2_4

Cited by 2 publications

(3 citation statements)

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“…46,47 Some microorganisms generate biosurfactants, such as rhamnolipids and polysaccharides, to emulsify immiscible substrates and enhance substrate accessibility. 32,48 direct contact between substrate droplets and motile cells to access substrates with limited aqueous phase solubility (i.e., decane, DFD), similar to what has been observed during hexadecane utilization by various Pseudomonas spp. 46,49 Proteins of the OprD family form channels to facilitate the uptake of a variety of substrates in Pseudomonas aeruginosa.…”

Section: And Biogenesis [J] Transcription [K] Signal Transduction Mec...mentioning

confidence: 53%

“…In decane- and DFD-grown cells, genes associated with the assembly of the flagellar motor ( fliEF , flgF , and flhA ) were upregulated, presumably in response to growth with water-immiscible substrates (Figure ). Motility allows the cells to actively move towards insoluble hydrocarbon droplets to access the substrate. , Some microorganisms generate biosurfactants, such as rhamnolipids and polysaccharides, to emulsify immiscible substrates and enhance substrate accessibility. , However, the genomic and transcriptomic analyses provided no evidence for the production of biosurfactants, suggesting strain 273 relies on direct contact between substrate droplets and motile cells to access substrates with limited aqueous phase solubility (i.e., decane, DFD), similar to what has been observed during hexadecane utilization by various Pseudomonas spp. , …”

Section: Discussionmentioning

confidence: 91%

“…Some microorganisms generate biosurfactants, such as rhamnolipids and polysaccharides, to emulsify immiscible substrates and enhance substrate accessibility.32,48 However, the genomic and transcriptomic analyses provided no evidence for the production of biosurfactants, suggesting strain 273 relies on Environ. Sci.…”

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confidence: 99%

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Genome-Wide Expression Analysis Unravels Fluoroalkane Metabolism in Pseudomonas sp. Strain 273

Xie,

Ramirez,

Chen

et al. 2023

Environ. Sci. Technol.

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Pseudomonas sp. strain 273 grows with medium-chain terminally fluorinated alkanes under oxic conditions, releases fluoride, and synthesizes long-chain fluorofatty acids. To shed light on the genes involved in fluoroalkane metabolism, genome, and transcriptome sequencing of strain 273 grown with 1,10-difluorodecane (DFD), decane, and acetate were performed. Strain 273 harbors three genes encoding putative alkane monooxygenases (AlkB), key enzymes for initiating alkane degradation. Transcripts of alkB-2 were significantly more abundant in both decane- and DFD-grown cells compared to acetate-grown cells, suggesting AlkB-2 catalyzes the attack on terminal CH3 and CH2F groups. Coordinately expressed with alkB-2 was an adjacent gene encoding a fused ferredoxin–ferredoxin reductase (Fd–Fdr). Phylogenetic analysis distinguished AlkB that couples with fused Fd–Fdr reductases from AlkB with alternate architectures. A gene cluster containing an (S)-2-haloacid dehalogenase (had) gene was up-regulated in cells grown with DFD, suggesting a possible role in the removal of the ω-fluorine. Genes involved in long-chain fatty acid biosynthesis were not differentially expressed during growth with acetate, decane, or DFD, suggesting the bacterium’s biosynthetic machinery does not discriminate against monofluoro-fatty acid intermediates. The analysis sheds first light on genes and catalysts involved in the microbial metabolism of fluoroalkanes.

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