Molecular and Field Analysis of Somaclonal Variation in Transgenic Plants

Sala, F.; Arencibia, Ariel D.; Castiglione, Stefano; Christou, Paul; Zheng, Youliang; Han, Yongxiang

doi:10.1007/978-94-011-4661-6_61

Cited by 12 publications

(8 citation statements)

References 9 publications

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“…However, increasing the number of subculturing cycles during tissue culture may result in genetic variation. It has been previously reported that the use of potential plant growth regulators like TDZ in tissue culture could induce alteration(s) in sensitive regions of the plant genome (Sala et al, 1999). ISSR markers are considered suitable to determine somaclonal variations among plants produced by micropropagation (Joshi and Dhawan, 2007;Leroy et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation and Detection of Somaclonal Variation in Phalaenopsis gigantea as Affected by Chitosan and Thidiazuron Combinations

Samarfard

Kadir²,

Kadzimin³

et al. 2014

horts

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Protocorm-like bodies (PLBs) multiplication is one of the most preferable in vitro methods to increase the number of orchids that produce very few seeds or seeds that are not able to germinate. In the present study the effects of chitosan and thidiazuron (TDZ) combinations on multiplication, differentiation, and genetic stability of Phalaenopsis gigantea PLBs were investigated using different media. Initial PLBs were cultured in solid New Dogashima (ND) medium and Vacin and Went (VW) medium supplemented with different concentrations of chitosan (0, 5, 10, 15, 20, and 25 mg·L–1) and TDZ (0, 0.1, and 0.5 mg·L–1). The highest mean number of PLBs (353 PLBs) was observed in ND medium with 10 mg·L–1 chitosan and 0.1 mg·L–1 TDZ combination after 20 weeks of culture. Some PLBs differentiated into mature PLBs with a profusion of leaves on the apical region, and tiny plantlets started to develop after 10 weeks of culture. The highest mean number of shoots was observed in VW supplemented with 10 mg·L–1 chitosan and 0.5 mg·L–1 TDZ (16 shoots). Intersimple sequence repeat (ISSR) markers were used to determine the genetic fidelity among mother plant and PLBs obtained from each subculture stage of solid ND medium supplemented with 10 mg·L–1 chitosan and 0.1 mg·L–1 TDZ (the optimal treatment for PLB proliferation). Dissimilarity of 5% occurred between the mother plant and PLBs obtained after 16 weeks of culture. The range in the similarity coefficient varied from 0.80 to 1.0, and only 20% dissimilarity occurred between mother plant and PLBs after 20 weeks of culture.

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Section: Discussionmentioning

confidence: 99%

In Vitro Propagation and Detection of Somaclonal Variation in Phalaenopsis gigantea as Affected by Chitosan and Thidiazuron Combinations

Samarfard

Kadir²,

Kadzimin³

et al. 2014

horts

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“…Extensive field testing is essential. Stable inheritance of a transgene in field trials has been reported in transgenic sugarcane previously (Gallo‐Meagher and Irvine, 1996; Arencibia et al, 1999; Sala et al, 1999), as well as in other graminaceous monocotyledons such as barley (Horvath et al, 2001) and rice (Jiang et al, 2000). However, the importance of thorough screening of transgenic plants that are propagated vegetatively was stressed in a paper on transgenic tall fescue (Bettany et al, 1998).…”

mentioning

confidence: 92%

“…Underlining the need for field data is the fact that the integrity and stability of the plant genome as a whole may be affected by the tissue culture and transformation process. Genomic change resulting from transgenesis has been documented in various plants including rice (Jiang et al, 2000; Labra et al, 2001), barley (Bregitzer et al, 1998; Choi et al, 2000) and sugarcane (Arencibia et al, 1999, 2000; Sala et al, 1999).…”

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confidence: 99%

Stability of Gene Expression and Agronomic Performance of a Transgenic Herbicide-Resistant Sugarcane Line in South Africa

Leibbrandt¹,

Snyman

2003

Crop Science

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Sugarcane (Saccharum hybrid) cultivar NCo310 was transformed with the pat gene, which confers resistance to the herbicide Buster (glufosinate ammonium; Bayer CropScience, Monheim am Rhein, Germany). The effect of the expression of this gene on the agronomic performance of a successful transgenic line, 22.2, was investigated. Field testing showed that the pat gene was stably expressed during three rounds of vegetative propagation. Morphological and agronomic characters such as stalk height, diameter, population, fiber, disease resistance, and yield, measured in the first ratoon, were not significantly different in the transgenic line and its untransformed counterpart. Of four weed treatments applied to transformed and untransformed plots, there were two scenarios in which significantly higher yields were observed: (i) untransformed cane treated using the conventional (non‐Buster) herbicide protocol, widely used for weed control in the South African sugar industry, and (ii) transformed cane treated with a conventional preemergence cocktail, followed by two 5‐L ha−1 Buster applications. However, the most economical weed control treatment is dependent on the cost of the herbicide to which resistance has been engineered. Buster currently costs (in South African Rand) ZAR150 (≈US $14.01) L−1 For Buster treatments to be cost effective for commercial growers, the price would have to be considerably lower. However, since hand‐hoeing is deleterious to the cane, small‐scale growers currently controlling weeds manually would find herbicide use an advantage. If they were to adopt transgenic sugarcane, Buster costs would need to be reduced only slightly for them to increase their returns.

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“…Use of synthetic plant growth regulators (PGRs) at suband supra-optimal levels was found to induce somaclonal variations in a number of crops (Martins et al 2004) as because, the presence of PGRs could induce alteration(s) in sensitive regions of the plant genome (Sala et al 1999) which can be attributed to DNA methylation, amplification or the activation of transposable elements (Brar and Jain 1998). It is, thus, necessary to establish the genetic stability of the regenerated plantlets under the influence of strong cytokinin, like TDZ, using molecular markers, viz., random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), etc.…”

Section: Introductionmentioning

confidence: 98%

Induction of multiple shoots in a monopodial orchid hybrid (Aerides vandarum Reichb.f × Vanda stangeana Reichb.f) using thidiazuron and analysis of their genetic stability

Kishor

Devi

2009

Plant Cell Tiss Organ Cult

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The effect of thidiazuron (TDZ) on direct multiple shoot induction in axenic seedlings of a monopodial orchid hybrid Aerides vandarum 9 Vanda stangeana, using a dual phase culture medium was studied. The culture system consisted of a basal agar solidified halfstrength Murashige and Skoog medium overlaid by a liquid fraction of the same composition. Highest regeneration of multiple shoots (15.8 shoots per seedling) was obtained in the medium containing 2% sucrose (w/v) supplemented with 2 mgl -1 TDZ. Genetic stability of the regenerated shoots was assessed using random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), and restriction fragment length polymorphism of the PCR amplified (PCR-RFLP) nrITS region, as well as those of the coding (matK) and non-coding (trnL-F) regions of the cpDNA. Across the randomly selected mother plant and nine of its regenerated shoots, 2,680 bands were generated by 19 RAPD and 12 ISSR primers, exhibiting monomorphic banding profiles. Homogenous PCR-RFLP profiles were generated for nrITS using four restriction enzymes (REs), matK using five REs, and trnL-F using six REs. These molecular analyses showed no genomic alterations in all regenerated shoots obtained on medium containing 2 mgl -1 TDZ.

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Molecular and Field Analysis of Somaclonal Variation in Transgenic Plants

Cited by 12 publications

References 9 publications

In Vitro Propagation and Detection of Somaclonal Variation in Phalaenopsis gigantea as Affected by Chitosan and Thidiazuron Combinations

In Vitro Propagation and Detection of Somaclonal Variation in Phalaenopsis gigantea as Affected by Chitosan and Thidiazuron Combinations

Stability of Gene Expression and Agronomic Performance of a Transgenic Herbicide-Resistant Sugarcane Line in South Africa

Induction of multiple shoots in a monopodial orchid hybrid (Aerides vandarum Reichb.f × Vanda stangeana Reichb.f) using thidiazuron and analysis of their genetic stability

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