2012
DOI: 10.1007/978-1-62703-275-9_9
|View full text |Cite
|
Sign up to set email alerts
|

Abstract: Fourier transform infrared (FTIR) spectroscopy is widely used in structural characterization of proteins or peptides. While the method does not have the capability of providing the precise, atomic-resolution molecular structure, it is exquisitely sensitive to conformational changes occurring in proteins upon functional transitions or upon intermolecular interactions. Sensitivity of vibrational frequencies to atomic masses has led to development of "isotope-edited" FTIR spectroscopy, where structural effects in… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

5
69
0

Year Published

2013
2013
2022
2022

Publication Types

Select...
3
2
2

Relationship

0
7

Authors

Journals

citations
Cited by 71 publications
(74 citation statements)
references
References 115 publications
5
69
0
Order By: Relevance
“…A positive ⌬ATR profile in the amide IЈ region and a negative ⌬ATR profile in the amide IIЈ band indicate that the helix axis is oriented parallel to the normal of the ATR plate and hence adopts a transmembrane orientation; on the contrary, a negative ⌬ATR amide IЈ band and a positive ⌬ATR amide IIЈ band indicate that the helix axis adopts an orientation close to the plane of the ATR plate and hence adopts an in-plane orientation on the membrane surface (43)(44)(45)(46).…”
Section: R Atrmentioning
confidence: 99%
See 2 more Smart Citations
“…A positive ⌬ATR profile in the amide IЈ region and a negative ⌬ATR profile in the amide IIЈ band indicate that the helix axis is oriented parallel to the normal of the ATR plate and hence adopts a transmembrane orientation; on the contrary, a negative ⌬ATR amide IЈ band and a positive ⌬ATR amide IIЈ band indicate that the helix axis adopts an orientation close to the plane of the ATR plate and hence adopts an in-plane orientation on the membrane surface (43)(44)(45)(46).…”
Section: R Atrmentioning
confidence: 99%
“…As a complementary procedure, we quantitatively interpreted the dichroic ratios to determine the angle of the secondary structure axis with respect to the normal of the membrane plane from the orientational order parameter, S, and the dichroic ratio, R ATR , at the wave numbers of the helix (43,44,(47)(48)(49). The measured order parameter S is expressed as a set of uniaxial S distributions,…”
Section: R Atrmentioning
confidence: 99%
See 1 more Smart Citation
“…In order to understand, at the molecular level, the adsorption process of proteins the application of surface characterizing analytical techniques sensitive to the protein structure in situ at the solid|liquid interface is required. Indeed, IRS is a superb technique allowing studies of the secondary structure of proteins and its changes [14,15,163,164]. PM IRRAS is sensitive to the structure of species adsorbed at a solid surface and it has been used to study the adsorption of proteins at stainless steel|air [162] and gold|air interfaces [165,166].…”
Section: Application Of In Situ Pm Irras For the Determination Of Thementioning
confidence: 99%
“…Determination of the structure of molecules adsorbed on electrode surfaces requires a use of spectroscopic and/or microscopic methods combined with electrochemical techniques. Among spectroscopic techniques, infrared spectroscopy (IRS) belongs to one of the most powerful bioanalytical techniques for studies of the molecular structure of biomolecules [12][13][14][15]. It gives the unique opportunity to analyze simultaneously each kind of organic species present in an assembly adsorbed on the electrode surface.…”
Section: Introductionmentioning
confidence: 99%