Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance

Bueno-Sancho, Vanessa; Bunting, Daniel C. E.; Yanes, Luis; Yoshida, Kentaro; Saunders, Diane G. O.

doi:10.1007/978-1-4939-7249-4_2

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…One example is ''field pathogenomics'', where RNA-sequencing data are generated from pathogen-infected leaf samples collected in the field. Phylogenetic and structural analyses based on the most variable SNPs within the pathogen population are employed to gain insight into the genetic groups present (Hubbard et al, 2015;Bueno-Sancho et al, 2017). This has been used to demonstrate population shifts in wheat yellow rust (Puccinia striiformis f. sp.…”

Section: Molecular Plantmentioning

confidence: 99%

Creation and judicious application of a wheat resistance gene atlas

et al. 2021

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Disease-resistance (R) gene cloning in wheat (Triticum aestivum) has been accelerated by the recent surge of genomic resources, facilitated by advances in sequencing technologies and bioinformatics. However, with the challenges of population growth and climate change, it is vital not only to clone and functionally characterize a few handfuls of R genes, but also to do so at a scale that would facilitate the breeding and deployment of crops that can recognize the wide range of pathogen effectors that threaten agroecosystems. Pathogen populations are continually changing, and breeders must have tools and resources available to rapidly respond to those changes if we are to safeguard our daily bread. To meet this challenge, we propose the creation of a wheat R-gene atlas by an international community of researchers and breeders. The atlas would consist of an online directory from which sources of resistance could be identified and deployed to achieve more durable resistance to the major wheat pathogens, such as wheat rusts, blotch diseases, powdery mildew, and wheat blast. We present a costed proposal detailing how the interacting molecular components governing disease resistance could be captured from both the host and the pathogen through biparental mapping, mutational genomics, and whole-genome association genetics. We explore options for the configuration and genotyping of diversity panels of hexaploid and tetraploid wheat, as well as their wild relatives and major pathogens, and discuss how the atlas could inform a dynamic, durable approach to R-gene deployment. Set against the current magnitude of wheat yield losses worldwide, recently estimated at 21%, this endeavor presents one route for bringing R genes from the lab to the field at a considerable speed and quantity.

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Section: Molecular Plantmentioning

confidence: 99%

Creation and judicious application of a wheat resistance gene atlas

et al. 2021

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“…Stewardship plans will thus need to be in place to deploy synthetic NLRs in combination and/or rotation with other resistance genes to prevent rapid resistance breakdown. Additionally, diagnostic methods such as field pathogenomics [ 79 , 80 ] or Marple [ 81 ] can identify pathogen isolates causing outbreaks and thus inform targeted engineering of new resistances.…”

Section: Discussionmentioning

confidence: 99%

Show me your ID: NLR immune receptors with integrated domains in plants

Marchal

Michalopoulou

Zou

et al. 2022

Essays in Biochemistry

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Nucleotide-binding and leucine-rich repeat receptors (NLRs) are intracellular plant immune receptors that recognize pathogen effectors secreted into the plant cell. Canonical NLRs typically contain three conserved domains including a central nucleotide binding (NB-ARC) domain, C-terminal leucine-rich repeats (LRRs) and an N-terminal domain. A subfamily of plant NLRs contain additional noncanonical domain(s) that have potentially evolved from the integration of the effector targets in the canonical NLR structure. These NLRs with extra domains are thus referred to as NLRs with integrated domains (NLR-IDs). Here, we first summarize our current understanding of NLR-ID activation upon effector binding, focusing on the NLR pairs Pik-1/Pik-2, RGA4/RGA5, and RRS1/RPS4. We speculate on their potential oligomerization into resistosomes as it was recently shown for certain canonical plant NLRs. Furthermore, we discuss how our growing understanding of the mode of action of NLR-ID continuously informs engineering approaches to design new resistance specificities in the context of rapidly evolving pathogens.

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