Activation of Transcription by the Cyclic AMP Receptor Protein

Crombrugghe, Benoít de; Busby, Stephen; Buc, Henri

doi:10.1007/978-1-4757-4619-8_4

Cited by 48 publications

(26 citation statements)

References 94 publications

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“…Positive transcriptional activators bind to unique target DNA sequences, forming stable, biochemically detectable protein-DNA complexes which are thought to function by directing precise transcriptional initiation by RNA polymerase (8,18,29,38,40). To determine the precise site of action of NodDl and NodD3 in the nod gene regulatory regions, we used both enzymatic and chemical nuclease footprinting of restriction fragments containing the nodA, nodF, and nodH promoters.…”

Section: Resultsmentioning

confidence: 99%

DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters

Fisher

Long

1989

J Bacteriol

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Section: Resultsmentioning

confidence: 99%

DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters

Fisher

Long

1989

J Bacteriol

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“…In gal the major groove contacts that CRP makes at -35 and -37 would prevent the polymerase from interacting there. Furthermore, the segment between -34 and -38 on the lower strand contains the sequence 5'TGTGA3', the consensus sequence found in the 5' half of CRP binding sites (6). RNA polymerase must, therefore, interact differently in this region at the lac UV5 promoter and at the gal y promoter.…”

Section: Discussionmentioning

confidence: 99%

“…DNAase I footprint experiments indicate that the interaction site for cAMP-CRP on gal DNA is located between -50 and -30 (the P mRNA start site is at +1) (4). The location of this site is different from its location in the lactose operon (between -70 and -50) (5) and in ott.er Nucleic Acids Research cyclic AMP dependent genes (6). The gal CRP binding site has been further defined by chemical protection experiments (4) and by the isolation of CRP binding site mutants (3).…”

Section: Introductionmentioning

confidence: 99%

Interactions of RNA polymerase and the cyclic AMP receptor protein on DNA of theE. coligalactose operon

Taniguchi

Crombrugghe

1983

Nucl Acids Res

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We have examined the interaction site on gal DNA for the cyclic AMP receptor protein and RNA polymerase when both are present together to form a stable initiation complex at the P gal promoter. Substitution of the bases to the left of -60 by unrelated DNA sequences does not change the cyclic AMP concentration dependency for in vitro transcription at P and inhibition of E2. Although the presence of some DNA to the left of -60 appears to be needed for efficient in vitro transcription at , the gal sequence to the left of -60 does not provide any specific interactions for transcription initiation at Pl. Similarly, efficient in vitro transcription from .2 also requires non-specific DNA sequences to the left of -60. We have also examined which bases were protected by RNA polymerase and CRP together from the action of DNAase and dimethylsulfate. Some of the interactions that take place when cAMP-CRP alone interacts with gal DNA appear to be preserved in the cAMP-CRP-RNA polymerase-gal DNA complex, suggesting that CRP occupies the same site in the DNA when it is alone or together with RNA polymerase. Our results suggest that the formation of an open complex at different promoters can result from different interaction patterns between RNA polymerase and promoter DNA.

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“…A CAP consensus sequence that strongly resembles that from E. coli lacZp has been identified near hutUp (12,22), and thus we tested whether CAPK would bind to lacZp and hutUp similarly. When CAPK was mixed with an equimolar mixture of three DNA fragments, one carrying lacZp, one carrying hutUp, and one carrying no known CAP binding sites, both the lacZp-and the hutUp-containing DNA fragments showed binding at CAPK concentrations as low as 15 to 20 nM, and complete binding was observed at 30 to 40 nM (Fig.…”

Section: ----------T--g--t ----T----t--t--c---c-g -----------------C mentioning

confidence: 99%

Klebsiella aerogenes catabolite gene activator protein and the gene encoding it (crp)

Osuna

Bender

1991

J Bacteriol

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The catabolite gene activator protein from Klebsiella aerogenes (CAPK) and the corresponding protein from Escherichia coli (CAPE) were shown to be nearly identical. Both CAPK and CAPE activated transcription from the CAP-dependent promoters derived from E. coli and K. aerogenes. The crp gene from K. aerogenes (encoding CAP) is tightly linked to rpsL. The nucleotide sequence of crp predicts an amino acid sequence for CAPK that differs in only one position from that of CAPE.

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Activation of Transcription by the Cyclic AMP Receptor Protein

Cited by 48 publications

References 94 publications

DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters

DNA footprint analysis of the transcriptional activator proteins NodD1 and NodD3 on inducible nod gene promoters

Interactions of RNA polymerase and the cyclic AMP receptor protein on DNA of theE. coligalactose operon

Klebsiella aerogenes catabolite gene activator protein and the gene encoding it (crp)

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