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Cited by 8 publications
(5 citation statements)
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“…Immunocytochemical methods. The hCG-like immunoreactive material was detected at the light microscope level by use of the indirect fluorescein-labeled and the indirect peroxidase-labeled reactions by procedures previously described in detail (2). For examination, 1 drop of sterile water was placed in each of the circles of a Fluoroslide (Curtin Matheson Scientific, Inc., Cleveland, Ohio), and then the surface(s) of one or several colonies of the respective microorganisms was gently touched with a bacteriological loop which was mixed in the drop of water in each circle.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Immunocytochemical methods. The hCG-like immunoreactive material was detected at the light microscope level by use of the indirect fluorescein-labeled and the indirect peroxidase-labeled reactions by procedures previously described in detail (2). For examination, 1 drop of sterile water was placed in each of the circles of a Fluoroslide (Curtin Matheson Scientific, Inc., Cleveland, Ohio), and then the surface(s) of one or several colonies of the respective microorganisms was gently touched with a bacteriological loop which was mixed in the drop of water in each circle.…”
Section: Methodsmentioning
confidence: 99%
“…Our immunocytochemical studies showed that the expression of the hCG-like material appeared to be a strain, not a species, characteristic. Moreover, the synthesis of the hCG-like material occurs in bacterial species belonging to the indigenous flora of humans (2,5,7). Of the limited number of bacteria studied, several expressing the hCG-like antigen are coagulase-negative staphylococci.…”
mentioning
confidence: 99%
“…The specificity of the antisera to hCGP that we have used has been well established, not only by all the usual controls for immunocytochemistry, but also by our demonstration of the absence of cross-reactivity between the antisera and cholera toxin (Acevedo et al, 1980), and by our demonstration of the lack of immunological analogy between the P-subunits of cholera toxin and hCGP in spite of their structural relatedness, the common CAGY segment (Acevedo & Kellen, 1985).…”
Section: Discussionmentioning
confidence: 86%
“…Using sensitive and specific assay methods a systematic search has revealed the presence of CG-like material in neoplastic cells of all experimental animal tumors that have been tested in vitro and in vivo [4], in a wide variety of cultured cells, sections and/or touch imprints of human malignant neoplasms [8,12,16], in spermatozoa [1,5] and in several strains of some species of bacteria isolated from cancer patients [2,3,11].…”
Section: Introductionmentioning
confidence: 99%