In situ Hybridization Histochemistry as a Tool for the Study of Brain Function

Baldino, Frank; Roberts-Lewis, Jill M.; Lewis, Michael

doi:10.1007/978-1-349-12275-2_1

Cited by 2 publications

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“…ISH histochemistry with synthetic oligonu cleotide and cRNA probes has greatly ad vanced the study of gene regulation in the CNS [Baldino et al, 1993b], The nonradioac tive methods described here are of sufficient sensitivity and resolution to detect both abun dant and relatively rare transcripts within in dividual cells of the CNS. cRNA probes are advantageous for detecting low-abundance mRNAs because they can be labeled to a much greater extent, while synthetic oligonu cleotide probes have the advantage of not requiring molecular biological expertise to ob tain and label.…”

Section: Discussionmentioning

confidence: 99%

Enzyme Histochemical Detection of Specific Neuronal mRNAs in Brain

Robbins¹,

Baldino²,

Roberts-Lewis³

et al. 1993

Cell Physiol Biochem

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The study of gene expression has been greatly facilitated by many recent advances in the technology of in situ hybridization histochemistry. The purpose of this report is to familiarize investigators with the basic principles of this technique and illustrate its potential with chosen examples in the field of neurobiology. A further goal is to provide insight into the specific options available in the selection of probes for use in non-radioactive (e.g. enzyme histochemical) detection of messenger RNA. Since many species differences should be apparent at the level of gene expression, this technology has considerable potential to aid in comparative studies of the nervous system.

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Section: Discussionmentioning

confidence: 99%