Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells

Srinivas, Priya; Gopinath, Gokul; Banerji, Asoke; Dinakar, Abhilash; Srinivas, G

doi:10.1002/mc.20031

Cited by 166 publications

(125 citation statements)

References 29 publications

(42 reference statements)

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“…ROS may change the redox state of cells leading to cell apoptosis through multiple pathways (41,49). ROS may also adjust Bax expression, a phenomenon that was evident in our study.…”

Section: Discussionsupporting

confidence: 64%

“…The induction of apoptosis by PL in a human cervical cancer cell line was also correlated with ROS generation (41). We assumed that PL induces apoptosis in osteosarcoma cell lines by generating ROS.…”

Section: Pl Increases the Generation Of Rosmentioning

confidence: 99%

“…PL was found to induce ROS generation in various types of cancer cells, such as human promyelocytic leukemia (40), human prostate cancer (18) and human cervical cancer cells (41).…”

Section: Introductionmentioning

confidence: 99%

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Plumbagin induces apoptosis via the p53 pathway and generation of reactive oxygen species in human osteosarcoma cells

et al. 2011

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Abstract. Osteosarcoma, which is the most common primary bone tumor, occurs most frequently in adolescents. A number of studies have indicated that plumbagin (PL) (5-hydroxy-2-methyl-1, 4-naphthoquinone), a compound found in the plants of the Plumbaginaceae and Droseraceae families, possesses anticancer activity. However, its anticancer effects and mechanisms against osteosarcoma have not been explored. To determine the anticancer effect of PL on osteosarcoma cell lines MG-63 and U2OS, cell viability, apoptosis, cell cycle distribution, caspase-3 and caspase-9 activity and intracellular reactive oxygen species (ROS) generation were measured, and Western blot analyses were performed. PL significantly inhibited the growth of osteosarcoma cells, particularly U2OS cells. PL up-regulated the expression of p53 in U2OS cells and p21 in the two osteosarcoma cell lines causing cell cycle arrest by decreasing the expression of murine double minute 2 (MDM2)/ cyclin B1 and cyclin D1. Furthermore, PL altered the ratio of Bax/Bcl-2, and may have triggered the mitochondrial apoptotic pathway, resulting in caspase-3 and caspase-9 activation. We also found that PL induced the generation of ROS in osteosarcoma cell lines. To conclude, PL exerted anticancer activity on osteosarcoma cells by inducing pro-apoptotic signaling and modulating the intracellular ROS that causes induction of apoptosis. These effects may relate to the p53 status.

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“…ROS may change the redox state of cells leading to cell apoptosis through multiple pathways (41,49). ROS may also adjust Bax expression, a phenomenon that was evident in our study.…”

Section: Discussionsupporting

confidence: 64%

Section: Pl Increases the Generation Of Rosmentioning

confidence: 99%

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Plumbagin induces apoptosis via the p53 pathway and generation of reactive oxygen species in human osteosarcoma cells

et al. 2011

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“…17 The antitumor effects of plumbagin have been shown in several cancers including squamous cell carcinoma, 18 leukemia 19 and myeloma, 15 as well as breast, 17,20 ovarian, 21 pancreatic, 22 lung, 23 liver 24 and cervical cancers. 25 In bone, breast cancer cells increase bone resorption by producing factors such as the parathyroid hormone-related protein (PTHrP) and interleukins that stimulate the osteoblasts and increase the production of the receptor activator of NF-kB (RANK) ligand (RANKL). RANKL interacting with its receptor RANK in osteoclast precursors activates the NF-kB signaling pathway inducing osteoclastogenesis.…”

Section: Plumbaginmentioning

confidence: 99%

Plant-derived anticancer agents: a promising treatment for bone metastasis

Juàrez¹

2014

BoneKEy Reports

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Bone metastasis is a very frequent complication of advanced cancer, and it remains an incurable disease. Current therapies that have been approved for the treatment of bone metastases delay the occurrence of skeletal-related events and can extend the patient's lifespan by a few years. However, they will not cure or cause the regression of established bone metastases, and new side effects are emerging after prolonged treatment. Thus, new therapies are severely needed. There are compelling evidences from in vitro and in vivo preclinical studies that support the use of compounds derived from plants to treat several forms of cancers including bone metastasis. More than 25% of the drugs used during the past 20 years were directly derived from plants, whereas another 25% are chemically altered natural products. Still, only 5-15% of the B250 000 higher plants have ever been investigated for bioactive compounds. There is a growing interest for the study of anticancer drugs with relatively low side effects that target specific key signaling pathways that control the establishment and progression of the cancer metastasis. Therefore, further studies are needed to identify new natural compounds with high efficiency in cancer prevention and treatment. Extensive reviews about plant-derived agents and their use in cancer have been published, but none when it comes to the treatment of bone metastases. Only a few of these compounds have been evaluated for the treatment of bone metastasis; here we describe some of the most prominent ones that are having the potential to reach the clinic soon.

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“…5 mg /kg body weight in albino rats. The Plumbagin, a quinonoid constituent isolated from the root of PZ has been shown to exert anti carcinogenic effect (Srinivas et al, 2004). PZ exhibits an inhibitory effect on carcinogenesis in the intestines, causes cytogenetic and cell cycle changes in mouse and it is also effective in Ehrlich ascites carcinoma, and possesses proliferation activity in human cervical cancer cells (Srinivas et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Effect of Plumbago zeylanica administration on brain neurotransmitter level in Wistar albino rats

Baskaran¹,

Shanmugam²,

Raman³

2015

J App Pharm Sci

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According to WHO about 80% of the world's population relies on traditional medicine for their primary health care. Plumbago zeylanica L. is a medicinal plant, belong to the family of Plumbaginaceae and the root of P. zeylanica contains several bioactive constituent like L-dopa, Plumbagin (naphthoquinone), droseron, chitranone, triterpenoid, anthraquinone. This study is designed to evaluate the effect of P. zeylanica and naphthoquinone on the level of various amine neurotransmitters namely epinephrine, norepinephrine, serotonin, 5-hydroxyindole 3-acetic acid (5-HIAA), and dopamine on the discrete regions of the brain tissues. Wistar male albino rats were treated separately with ethanoloic extract of P. zeylannica (root) and the commercially purchased phytochemical naphthoquinone at the dose of 2mg/kg b. wt with different experimental groups. The brain tissue homogenates (cerebral cortex, cerebellum, hypothalamus, pons-medulla, midbrain, and corpus striatum) were analyzed to quantify the aforementioned neurotransmitters by high performance liquid chromatography (HPLC). The results showed that, administration of P. zeylanica and NQ does not alter the many of the studied neurotransmitter at significant level; however, there is a change in the neurotransmitter profile in few specific regions of Wistar rat brain and striatum was found to be affected more.

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Plumbagin induces reactive oxygen species, which mediate apoptosis in human cervical cancer cells

Cited by 166 publications

References 29 publications

Plumbagin induces apoptosis via the p53 pathway and generation of reactive oxygen species in human osteosarcoma cells

Plumbagin induces apoptosis via the p53 pathway and generation of reactive oxygen species in human osteosarcoma cells

Plant-derived anticancer agents: a promising treatment for bone metastasis

Effect of Plumbago zeylanica administration on brain neurotransmitter level in Wistar albino rats

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