IONP‐PLL: a novel non‐viral vector for efficient gene delivery

Xiang, Juanjuan; Tang, Jingqun; Zhu, Shuchai; Nie, Xin; Lü, Hongbin; Shen, Shourong; Li, Xiaoling; Tang, Ke; Zhou, Ming; Li, Guiyuan

doi:10.1002/jgm.419

Cited by 85 publications

(72 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Characteristics of IONP-PLL and IONP-PLL/DNA complexes IONP-PLL were prepared according to Xiang et al 17,18,35 The structure and dimensions of IONP-PLL/DNA complexes are shown in Figure 1. IONP-PLL/DNA complexes are 48 ± 14 nm in diameter.…”

Section: Resultsmentioning

confidence: 99%

“…Exogenous gene distribution in tumor metastatic nodules in a pulmonary metastasis model In a previous study, 18 we defined the organ distribution of the gene expression by using flow cytometry after intravenous injection of IONP-PLL/EGFP-C2 complexes to mice. Single-cell suspensions of organs were prepared and the percentage of GFP-positive cells was assessed.…”

Section: Resultsmentioning

confidence: 99%

“…In earlier studies, 17,18,35 we have developed a non-viral vector, IONP-PLL, that could bind and protect DNA when incubated with DNase-I or serum. These particles showed high transfection efficiency in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon resulted not only from the ability of IONP-PLL to prevent enzymatic degradation of plasmid DNA in vivo but also from the long circulation time of the particles, which creates a longer exposure of the tumor. 18 It is noteworthy that the combination treatment of cyclophosphamide and the NM23-H1-GFP gene loaded IONP-PLL induced a more efficient suppression of metastasis and longer survival time of mice. Other researchers also have shown improved antitumoral effects of gene therapy in combination with chemotherapy in animal models.…”

Section: To Investigate the Distribution And Expression Of The Nm23-hmentioning

confidence: 96%

“…[13][14][15][16] We have developed a non-viral nanovector, IONP-PLL (poly-L-lysine-modified iron oxide nanoparticle), 17 which consists of iron oxide nanoparticles conjugated to poly-L-lysine. An earlier study 18 has shown that IONP-PLL nanoparticles have a diameter of about 58 nm, bind and protect DNA, show high transfection efficiency in vitro and in vivo and are highly biocompatible. IONP-PLL/DNA complexes intraveneously injected into mice are distributed into organs, including the brain, spleen and kidneys, with the most efficient delivery to the lungs.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Xiang

Zhang

et al. 2008

Cancer Gene Ther

View full text Add to dashboard Cite

Gene therapy provides a promising approach for cancer treatment. Earlier studies suggested that poly-L-lysine-modified iron oxide nanoparticles (IONP-PLL) might be a promising gene delivery system that can transfect DNA efficiently in vitro and in vivo. In this study we used IONP-PLL as gene carriers to deliver the NM23-H1 gene, the first suppressor gene of cancer metastasis, to tumor cells in vivo. The intravenous injection of IONP-PLL carrying NM23-H1-GFP plasmid DNA significantly extended the survival time of an experimental pulmonary metastasis mouse model. In the IONP-PLL/NM23-H1-GFP-treated group, metastasis was clearly suppressed compared with the group treated with free NM23-H1-GFP plasmid. Furthermore, this gene therapy combined with cyclophosphamide treatment resulted in longer survival times and greater suppression of metastasis growth. In conclusion, treatment with IONP-PLL nanoparticles incorporating the NM23-H1gene is an efficient gene therapy method, and it is even more effective in combination with chemotherapy. This approach appears to be a promising strategy for treatment of metastatic tumors.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: To Investigate the Distribution And Expression Of The Nm23-hmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Xiang

Zhang

et al. 2008

Cancer Gene Ther

View full text Add to dashboard Cite

show abstract

Catalytic Transfer of Chiral Information from an Organic Compound to a Coordination Complex

Fontecave

2010

ChemCatChem

View full text Add to dashboard Cite

show abstract

Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity

Valdiglesias

Kılıç

Costa

et al. 2014

Environ and Mol Mutagen

131

View full text Add to dashboard Cite

Iron oxide nanoparticles (ION) with superparamagnetic properties hold great promise for use in various biomedical applications; specific examples include use as contrast agents for magnetic resonance imaging, in targeted drug delivery, and for induced hyperthermia cancer treatments. Increasing potential applications raise concerns over their potential effects on human health. Nevertheless, very little is currently known about the toxicity associated with exposure to these nanoparticles at different levels of biological organization. This article provides an overview of recent studies evaluating ION cytotoxicity, genotoxicity, developmental toxicity and neurotoxicity. Although the results of these studies are sometimes controversial, they generally indicate that surface coatings and particle size seem to be crucial for the observed ION-induced effects, as they are critical determinants of cellular responses and intensity of effects, and influence potential mechanisms of toxicity. The studies also suggest that some ION are safe for certain biomedical applications, while other uses need to be considered more carefully. Overall, the available studies provide insufficient evidence to fully assess the potential risks for human health related to ION exposure. Additional research in this area is required including studies on potential long-term effects.

show abstract

IONP‐PLL: a novel non‐viral vector for efficient gene delivery

Abstract: IONP-PLL, a low-toxicity vector, appears to have potential for fundamental research and genetic therapy in vitro and in vivo, especially for gene therapy of CNS disease.

Cited by 85 publications

References 34 publications

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Nanoparticle delivery of anti-metastatic NM23-H1 gene improves chemotherapy in a mouse tumor model

Catalytic Transfer of Chiral Information from an Organic Compound to a Coordination Complex

Effects of iron oxide nanoparticles: Cytotoxicity, genotoxicity, developmental toxicity, and neurotoxicity

Contact Info

Product

Resources

About