Recent Advances in CRISPR‐Cas9 Genome Editing Technology for Biological and Biomedical Investigations

Abstract

The Type II CRISPR-Cas9 is a simple, efficient, and versatile technology for targeted genome editing in a wide range of organisms and cell types. It continues to gain more scientific interest and has established itself as an extremely powerful technology within our synthetic biology toolkit. It works upon a targeted site and generates a double strand breaks that become repaired by either the NHEJ or the HDR pathway, modifying or permanently replacing the genomic target sequences of interest. These can include viral targets, single-mutation genetic diseases, and multiple-site corrections for wide scale disease states, offering the potential to manage and cure some of mankind's most persistent biomedical menaces. Here, we present the developing progress and future potential of CRISPR-Cas9 in biological and biomedical investigations, toward numerous therapeutic, biomedical, and biotechnological applications, as well as some of the challenges within. J. Cell. Biochem. 119: 81-94, 2018.

Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells

“…These hard-to-modify cells would highly benefit from new delivery methods. Consequently, we investigated the potential of 6His-CM18-PTD4 to deliver CRISPR-Cas9 and -Cpf1 RNPs to human T-cell-derived Jurkat and primary NK cells [ 32 , 33 ].…”

Antimicrobial Resistance: Its Surveillance, Impact, and Alternative Management Strategies in Dairy Animals

“…These are adaptive immune systems native to bacteria and archaea and can be used for sequence-specific killing of target bacterial strains ( 241 ). It works on a selective site and creates a double stranded nick in the DNA, modifying or permanently replacing the target sequence ( 242 ). Few other studies have reported the successful application of CRISPR-Cas in managing antibiotic resistance and reversing the selection pressure ( 243 , 244 ).…”

“…The DNA carries CRISPR, a DNA-editing technology offer that cuts away the antibiotic-resistant genes. Recent developments in CRISPR-Cas genome editing technology and its potential application in food bacteria has been recently reviewed elsewhere ( 242 , 246 ). CRISPR-Cas technology can have potential application in controlling AMR at dairy farms, through application of developed product, spray, or liquid, etc., in dairy farm envirnonment, dairy personnel hands, etc.…”

Book Review: Quorum Sensing vs. Quorum Quenching: A Battle With No End in Sight

“…Recent breakthroughs in targeted genome engineering by CRISPR-Cas9 (Jinek et al, 2012 , 2013 ; Mali et al, 2013 ; Singh et al, 2017 , 2018 ) has opened new avenues for genome editing and regulation in a wide range of organisms and cell types. In this light, enabling the use of CRISPR for the modification of bacterial genomes (Jiang et al, 2013 ) has opened the way toward the development of sequence-specific CRISPR-based antimicrobials (Beisel et al, 2014 ; Bikard et al, 2014 ; Bikard and Barrangou, 2017 ).…”