2006
DOI: 10.1002/elan.200503418
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Abstract: Gold modified nanoporous silica based magnetic microparticles have been prepared as support for the immobilization of the enzyme horseradish peroxidase (HRP). The enzyme modified gold microparticles were retained onto the surface of a solid carbon paste electrode with the help of a permanent magnet. The analytical performances of the resulting biosensor were characterized by studying hydroquinone (HQ) and hydrogen peroxide. The former was monitored by the direct electroreduction of the biocatalytically generat… Show more

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Cited by 42 publications
(18 citation statements)
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“…This behavior permitted to suggest that the oxidation product NAPQI reacted with added GSH, following a Michael type reaction, to form the GS-APAP conjugate as illustrated in Scheme 1 [20,28,29].…”
Section: Inhibition Studiesmentioning
confidence: 96%
See 1 more Smart Citation
“…This behavior permitted to suggest that the oxidation product NAPQI reacted with added GSH, following a Michael type reaction, to form the GS-APAP conjugate as illustrated in Scheme 1 [20,28,29].…”
Section: Inhibition Studiesmentioning
confidence: 96%
“…APAP has been studied with biosensors based on polyphenol oxidase (from avocado tissue) [17], aryl acylamidase [18] and HRP [19] with amperometric detection of the generated NAPQI. Recently, we described the use of nanoporous magnetic microparticles (MMPs) for the construction of amperometric HRP immobilized biosensors [20,21]. The porous nanostructure of the microparticles permits a high enzyme loading; the beads can be attracted by a magnet and retained in close proximity to the electrode surface.…”
Section: Introductionmentioning
confidence: 99%
“…The carbon paste was packed into the hole of the electrode body and smoothed on a piece of clean paper until it had a shiny appearance. The electrode body was constructed by pressing a small rod of stainless-steel (diameter 2 mm) inside a micropipette tip (1 mL volume capacity), leaving a space at the surface tip of approximately 1 mm for housing the carbon paste; a thin wire was inserted through the opposite end to establish electrical contact [31]. The carbon paste electrode was immersed in the supporting electrolyte placed in the cell, and several sweeps were applied to obtain a low background current.…”
Section: Preparation Of the Carbon Paste Electrodementioning
confidence: 99%
“…The electrode body was constructed by pressing a small rod of stainless-steel (diameter 2 mm) inside a micropipette tip (1 ml volume capacity), leaving a depression at the surface tip approximately 1 mm for housing the carbon paste; a thin wire was inserted through the opposite end to establish electrical contact. 17 The carbon paste electrode was The anodic voltammetric behavior of tiapride hydrochloride (TiapCl) was studied at carbon paste electrodes in 0.04 M Britton-Robinson buffer pH 7.0 using cyclic and differential pulse voltammetric techniques. The oxidation of TiapCl is an irreversible diffusion-controlled process.…”
Section: Preparation Of Carbon Paste Electrodementioning
confidence: 99%