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Fu, Jinzhong; MacCulloch, Ross D.; Murphy, Robert W.; Darevsky, Ilya S.; Tuniyev, Boris S.

doi:10.1023/a:1004096330727

Cited by 15 publications

(10 citation statements)

References 16 publications

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“…Potentially, one could explain polymorphism within the parthenogens by the aggregation of de novo mutations. However, additional evidence caused multiple authors to interpret genetic variation within the parthenogens as polyclonality, i.e., descent from different F1 hybrids [ 48 , 49 , 51 , 52 , 54 , 55 ]; but see [ 53 ]. In our study, the presence of multiple and fixed differences between D. armeniaca and D. dahli is probably not solely the result of accumulated mutation; at least such extent of divergent phenotypic evolution is unknown in vertebrate parthenogens.…”

Section: Discussionmentioning

confidence: 99%

“…To check for contamination and pipetting error, a negative control (reagents only) was used for each extraction procedure and PCR. Two different primer pairs (H15915 – L15369 and H15488 – L15153, [ 45 , 48 ]) were used for amplification of a 683 bp fragment of the cytochrome b mitochondrial gene [ 24 , 45 , 46 ]. The PCR conditions were as follows: 20 μl total volume, with 2–4 μl template DNA, 1 U of GoTaq Flexi DNA Polymerase (Promega), 1X GoTaq Green Flexi DNA Polymerase buffer, 1 mM of MgCl2, 0.2 mM of each dNTP and primer concentrations at 0.1 μM.…”

Section: Methodsmentioning

confidence: 99%

“…Parker et al [ 47 ] did not exclude the occasional presence of more than one clonal hybrid lineage within the same taxon. Later publications also indirectly suggest polyclonality: Fu et al [ 48 ] discovered allozyme variation within D. unisexualis (three genotypes), D. uzzelli, and D. bendimahiensis (two genotypes each). Polymorphisms were also recorded for D. armeniaca [ 36 , 49 , 50 ], D. dahli [ 51 , 52 ], D. unisexualis [ 37 , 48 ], D. rostombekowi [ 53 ], D. uzzelli and D. bendimahiensis [ 48 ].…”

Section: Introductionmentioning

confidence: 99%

“…Later publications also indirectly suggest polyclonality: Fu et al [ 48 ] discovered allozyme variation within D. unisexualis (three genotypes), D. uzzelli, and D. bendimahiensis (two genotypes each). Polymorphisms were also recorded for D. armeniaca [ 36 , 49 , 50 ], D. dahli [ 51 , 52 ], D. unisexualis [ 37 , 48 ], D. rostombekowi [ 53 ], D. uzzelli and D. bendimahiensis [ 48 ]. Vergun et al [ 54 ], Ryskov et al [ 53 ], and Girnyk et al [ 55 ] examined the nucleotide sequence variation in amplicons containing the microsatellite repeats, in D. dahli , D. rostombekowi , and D. armeniaca .…”

Section: Introductionmentioning

confidence: 99%

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Genotypic similarities among the parthenogenetic Darevskia rock lizards with different hybrid origins

et al. 2020

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Background The majority of parthenogenetic vertebrates derive from hybridization between sexually reproducing species, but the exact number of hybridization events ancestral to currently extant clonal lineages is difficult to determine. Usually, we do not know whether the parental species are able to contribute their genes to the parthenogenetic vertebrate lineages after the initial hybridization. In this paper, we address the hypothesis, whether some genotypes of seven phenotypically distinct parthenogenetic rock lizards (genus Darevskia) could have resulted from back-crosses of parthenogens with their presumed parental species. We also tried to identify, as precise as possible, the ancestral populations of all seven parthenogens. Results We analysed partial mtDNA sequences and microsatellite genotypes of all seven parthenogens and their presumed ansectral species, sampled across the entire geographic range of parthenogenesis in this group. Our results confirm the previous designation of the parental species, but further specify the maternal populations that are likely ancestral to different parthenogenetic lineages. Contrary to the expectation of independent hybrid origins of the unisexual taxa, we found that genotypes at multiple loci were shared frequently between different parthenogenetic species. The highest proportions of shared genotypes were detected between (i) D. sapphirina and D. bendimahiensis and (ii) D. dahli and D. armeniaca, and less often between other parthenogens. In case (ii), genotypes at the remaining loci were notably distinct. Conclusions We suggest that both observations (i-ii) can be explained by two parthenogenetic forms tracing their origin to a single initial hybridization event. In case (ii), however, occasional gene exchange between the unisexual and the parental bisexual species could have taken place after the onset of parthenogenetic reproduction. Indeed, backcrossed polyploid hybrids are relatively frequent in Darevskia, although no direct evidence of recent gene flow has been previously documented. Our results further suggest that parthenogens are losing heterozygosity as a result of allelic conversion, hence their fitness is expected to decline over time as genetic diversity declines. Backcrosses with the parental species could be a rescue mechanism which might prevent this decline, and therefore increase the persistance of unisexual forms.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotypic similarities among the parthenogenetic Darevskia rock lizards with different hybrid origins

et al. 2020

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“…At present, it has been found that parthenogenesis in reptiles is known in less than 0.46% of species ( Fujita and Moritz 2009 ). In general, cytological, genetic, morphological and ecological studies have shown that parthenogenetic lizards of the genus Darevskia , namely D. dahli (Darevsky, 1957), D. rostombekowi (Darevsky, 1957), D. uzzelli (Darevsky et Danielyan 1977), D. armeniaca (Méhely, 1909), D. unisexualis (Darevsky, 1966), D. sapphirina (Schmidtler et al, 1994) and D. bendimahiensis (Schmidtler et al, 1994) have originated as a result of natural hybridisation between bisexual parental species ( Darevsky 1958 , Darevsky 1967 , Uzzell and Darevsky 1975 , Macculloch et al 1997 , Fu et al 1999 , Fu et al 2000a , Fu et al 2000b , Murphy et al 2000 , Kupriyanova 1999 , Martirosian et al 2002 , Martirosian et al 2003 , Malysheva et al 2007a , Malysheva et al 2007b ).…”

Section: Introductionmentioning

confidence: 99%

New records and geographic distribution of the sympatric zones of unisexual and bisexual rock lizards of the genus Darevskia in Armenia and adjacent territories

Petrosyan¹,

Osipov²,

Бобров³

et al. 2020

BDJ

785

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Caucasian rock lizards of the genus is a unique taxa, including both bisexual and parthenogenetic species. The parthenogenetic species have originated as a result of natural hybridisation between females and males of different bisexual species. The species involved in interspecific hybridisation are called parental. However, sympatric zones (SZ) of unisexual and bisexual rock lizards of the Caucasus are still poorly studied, although they are very important for understanding the role of hybrid individuals of different origin in reticulate evolution. This paper presents the location of the SZs of parthenogenetic and their parental bisexual rock lizards of the genus Darevskia in Armenia and adjacent territories of Georgia and Nagorno-Karabakh. We summarised the locations of the SZs identified from 1957 to the present, based on our field survey data gathered in 2018-2019 and records from publications and museum collections. This dataset includes 39 SZs of three types: SZ of parental bisexual species, SZ of parental species with unisexual species and SZ of the parthenogenetic species. For each zone, species composition, geographical and altitudinal distribution are presented. New records expand our knowledge of the geographical and altitudinal distribution of SZs in these species and provide additional data for understanding the mechanisms of reticulate evolution and hybridogeneous speciation in the past, present and future. The new records, including geographical and altitudinal distributions of three types of SZs, are presented, which expand the previously-known list to 39 locations of contact zones for parthenogenetic and its bisexual parental species of rock lizards of the genus Darevskia in Armenia and the adjacent territories of Georgia and Nagorno-Karabakh.

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Molecular characterization of allelic variants of (GATA)n microsatellite loci in parthenogenetic lizards Darevskia unisexualis (Lacertidae)

Korchagin

Badaeva

Tokarskaya

et al. 2007

Gene

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Cited by 15 publications

References 16 publications

Genotypic similarities among the parthenogenetic Darevskia rock lizards with different hybrid origins

Genotypic similarities among the parthenogenetic Darevskia rock lizards with different hybrid origins

New records and geographic distribution of the sympatric zones of unisexual and bisexual rock lizards of the genus Darevskia in Armenia and adjacent territories

Molecular characterization of allelic variants of (GATA)n microsatellite loci in parthenogenetic lizards Darevskia unisexualis (Lacertidae)

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