We studied the effect of a donor of peroxynitrite, SIN-1, on the morphological characteristics of interweaved rat С6 glioma cells, on menadione-induced production of superoxide anion radicals, and on the concentration of Са 2+ in these cells. In concentrations of 1.25·10 -4 to 2.5·10 -7 М, SIN-1 demonstrated cytotoxic and antimitogenic effects. This donor of peroxynitrite caused abnormal modifications of the size of С6 cells and the structure of cellular organelles, intensified in a dose-dependent manner the release of Са 2+ from cellular stores into the cytoplasm, and suppressed menadione-induced production of superoxide anion radicals. Therefore, it should be believed that peroxynitrite exerts a modifying effect on the processes of mitotic division and induces apoptosis; it is also involved in the processes of intracellular signalling providing an increase in the concentration of cytosolic Са 2+ and a decrease in the redox activity of cells.
Previously we have characterized a new oncogene CHI3L1, overexpressed in the glioblastoma and the malignant 293_CHI3L1 cells, stably producing the CHI3L1 angiogenic oncoprotein. The 293_CHI3L1 cells proliferated faster and acquired a higher ability for anchorage-independent growth. Here, we report the atomic force microscopy data and functional characteristics of these cells. The constitutive CHI3L1 expression leads to the increased resistance to the damages by oxidative substances and promotes the chromosome instability in the 293 cells. According to the data of the last clinical investigations, anti-cancer therapy should be targeted not at the individual genes, but at the pathological effects they caused. We propose a complex treatment of gliomas including multi-target inhibitors, which can be delivered to the brain tumor by a specific nanoparticle vector.
The synthesis of a new nanostructured supramolecular europium complex supported by graphene oxide (GO) as a structure‐forming site is herein presented. The bonding of Eu ions to the GO surface is proven by energy dispersive X‐ray spectroscopy and photoluminescence mapping, as well as scanning electron microscopy (SEM), spectroscopic, Fourier transform infrared spectroscopy, Raman, and luminescence measurements. Though GO is one of the ligands and absorbs light strongly, the energy transfer from GO to Eu ion is weak. The splitting of 5D0→7F2 peak and three‐component luminescence decay means that there are several emitting centers in this composite. The obtained data are the first proof of concept for a stable, highly luminescent, and electrically conductive hybrid nanocomposite based on polystyrene added with 2% (w/w) of the obtained Eu–GO complex. The simple and reliable proposed procedure is prone to be easily scaled up and open the route for the commercialization of this nanomaterial.
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