The level o f cross-tolerance o f triticale callus lines obtained both to salt and osmotic stress was analyzed. It was established that stability of expression o f the character in callus lines obtained was rather high -o f 50 to 76% calli survived to the end o f the sixth passage. Salt-and osmotolerant callus lines o f triticale demonstrated actively their growth on selective medium with sublethal concentrations o f mannitol and sodium chloride respectively. Triticale salt-tolerant cell lines were characterized with high content o f free proline too.
[1]. Öÿ êóëüòóðà ïîºäíóº â ñîá³ âè-ñîêèé ïîòåíö³àë óðîaeàéíîñò³ çåðíà òà çåëå-íî¿ ìàñè, êîìïëåêñíèé ³ìóí³òåò äî ãðèáíèõ çàõâîðþâàíü, âèñîêèé âì³ñò á³ëêà é ë³çèíó â çåðí³, à òàêîae îñíîâíèõ ïîaeèâíèõ ðå÷î-âèí ó çåëåí³é ìàñ³ [2, 3].Ó çâ'ÿçêó ç ïîñò³éíî çðîñòàþ÷èì ñâ³òîâèì ïîïèòîì íà ïðîäîâîëü÷å çåðíî òðèòèêàëå éîãî âèðîùóþòü ìàéaeå â óñ³õ ´ðóíòîâî-êë³-ìàòè÷íèõ çîíàõ Óêðà¿íè, äå ïîðÿä ç ³íøèìè ïðè÷èíàìè, ùî çíèaeóþòü éîãî âðîaeàéí³ñòü, çíà÷íî¿ øêîäè çàâäàþòü àá³îòè÷í³ ñòðåñîâ³ ÷èííèêè, çîêðåìà çàñîëåííÿ ´ðóíò³â [4, 5]. Øê³äëèâà ä³ÿ çàñîëåííÿ ìຠêîìïëåêñíèé õàðàêòåð ³ çóìîâëåíà ÿê ïîðóøåííÿì îñìî-òè÷íîãî áàëàíñó êë³òèíè, òàê ³ ïðÿìèì òîê-ñè÷íèì âïëèâîì íà ô³ç³îëîã³÷í³ òà á³îõ³ì³÷-í³ ïðîöåñè â êë³òèí³ [6, 7]. ßê â³äîìî [8][9][10][11], ñò³éê³ñòü ðîñëèí ïðîòè íåñïðèÿòëèâèõ ÷èííèê³â äîâê³ëëÿ º ãåíå-òè÷íî äåòåðì³íîâàíîþ ³ ïðîÿâëÿºòüñÿ íà ð³çíèõ ð³âíÿõ îðãàí³çàö³¿, çîêðåìà é íà êë³-òèííîìó. Öå äຠçìîãó âèêîðèñòîâóâàòè á³î-òåõíîëîã³÷í³ ï³äõîäè, ÿê³ áàçóþòüñÿ íà êë³-òèííèõ òåõíîëîã³ÿõ in vitro, ùî, ç îäíîãî áîêó, äຠçìîãó ðîçøèðèòè ãåíåòè÷íó ð³çíî-ìàí³òí³ñòü ðîñëèí, áåçïîñåðåäíüî âïëèâàþ-÷è íà ãåíåòè÷íèé àïàðàò, ç ³íøîãî -ñòâîðè-òè ñèñòåìè ïðÿìîãî äîáîðó ñò³éêèõ ãåíîòè-ï³â [9, 10]. Ïåðåâàãè äîáîðó in vitro, ïîð³â-íþþ÷è ç òðàäèö³éíèìè ìåòîäàìè, ïîëÿãà-þòü íàñàìïåðåä â åêîíî쳿 ì³ñöÿ òà ìîaeëè- Ìåòà. Ïðîâåñòè ñêðèí³íã in vitro ð³çíèõ ãåíîòèï³â òðèòèêàëå îçèìîãî íà ñò³éê³ñòü ïðîòè çàñîëåííÿ â êóëüòóð³ àï³êàëüíèõ ìåðèñòåì ïàãîí³â. Ìåòîäè. Êóëüòóðà òêàíèí ³ îðãàí³â in vitro, ñåëåêö³ÿ in vitro, ñòàòèñòè÷íèé àíàë³ç. Ðåçóëüòàòè. Âèÿâëåíî, ùî ç³ çá³ëüøåííÿì êîíöåíòðàö³¿ õëîðèäó íàòð³þ ç 0,6 äî 1,5% ó âñ³õ ãåíîòèï³â â³äáóâàëîñÿ ïðèãí³÷åííÿ ðîñòó êàëþñíî¿ êóëüòóðè, ùî ñâ³ä÷èòü ïðî òîêñè÷íèé âïëèâ ñòðåñîâîãî ÷èííèêà. Âñòàíîâëåíî, ùî êîíöåíòðàö³ÿ 1,2% õëîðèäó íàòð³þ äຠçìîãó äèôåðåíö³þâàòè ãåíîòèïè òðèòèêàëå çà ñîëåñò³éê³ñòþ. Âèçíà÷åíî, ùî íàéá³ëüøîþ ñò³éê³ñòþ ïðîòè ñîëüîâîãî ñòðåñó õàðàêòåðèçóâàëàñÿ ë³í³ÿ '38/1296', îñê³ëüêè êàëþñè öüîãî ãåíîòèïó â ñåëåêòèâíèõ óìîâàõ â³äð³çíÿëèñÿ ï³äâèùåíèì ìîðôîãåíåòè÷íèì ïîòåíö³àëîì, ìàëè íàéá³ëüøèé ïðèð³ñò ñèðî¿ ìàñè, ³ ëèøå ç åêñïëàíò³â ö³º¿ ë³í³¿ ï³ñëÿ êóëüòèâóâàííÿ íà ñåðåäîâèù³ ç õëîðèäîì íàòð³þ êîíöåíòðàö³ºþ 1,5% áóëî îòðèìàíî ðîñëèíè-ðåãåíåðàíòè. Ñîðò 'ÀÄÌ 11' âèÿâèâñÿ íàé÷óòëèâ³øèì äî ñîëüîâîãî ñòðåñó, òîìó ùî â éîãî êàëþñàõ â ñåëåê-òèâíèõ óìîâàõ áóëî âèÿâëåíî ìàñîâèé íåêðîç òà â³äñóòí³ñòü ðåãåíåðàö³éíî¿ çäàòíîñò³. Ó âèâ÷åíèõ ôîðì çàçíà÷åíî ãåíîòèïîâó çàëåaeí³ñòü ïðîöåñ³â ìîðôîãåíåçó â êóëüòóð³ in vitro. Ç ³íäóêîâàíèõ êàëþñ³â îòðèìàíî ðîñëèíè-ðåãåíåðàíòè, îïòèì³çîâàíî ¿õ äîðîùóâàííÿ, âêîð³íåííÿ òà ïåðåâåäåííÿ â óìîâè in vivo. Âèñíîâêè. Ãåíîòèïîâà ðåàêö³ÿ íà ñîëüî-âèé ñòðåñ ó êóëüòóð³ àï³êàëüíèõ ìåðèñòåì ïàãîí³â òðèòèêàëå îçèìîãî ïðîÿâëÿëàñÿ íåîäíàêîâèì ïðèðîñòîì ñèðî¿ ìàñè òà ð³çíèì ìîðôîãåíåòè÷íèì ïîòåíö³àëîì. ˳í³ÿ '38/1296' ìîaeå áóòè âèêîðèñòàíà ÿê ö³ííèé ìàòåð³àë äëÿ ïîäàëüøî¿ ñåëåêö³¿ òðèòèêàëå îçèìîãî. Êóëüòóðó àï³êàëüíèõ ìåðèñòåì ïàãîí³â ðåêîìåíäîâàíî çàñòîñîâóâàòè ÿê òåñò-ñèñòåìó äëÿ ïðîâåäåííÿ ñêðèí³íãó ãåíîòèï³â òðèòèêàëå íà ñò³éê³ñòü ïðîòè ñîëüîâîãî ñòðåñó.Êëþ÷îâ³ ñëîâà: òðèò...
ÂñòóïÇíà÷íèì äîñÿãíåííÿì ñó÷àñíî¿ ãåíåòèêè ³ ñåëåêö³¿ ðîñëèí º ñòâîðåííÿ òðèòèêàëå (×Triticosecale spp. Wittmack ex A.Camus 1927), ñîðòè ÿêîãî óñï³øíî âïðîâàäaeóþòüñÿ â ñ³ëüñüêîãîñïîäàðñüêå âèðîáíèöòâî [1]. Ðà-çîì ³ç òèì, íåçâàaeàþ÷è íà âèñîê³ ïîòåíö³éí³ ìîaeëèâîñò³, öÿ åâîëþö³éíî ìîëîäà êóëüòóðà íà öåé ÷àñ íå îòðèìàëà øèðîêîãî âèðîáíè-÷îãî âèêîðèñòàííÿ [2]. Íåäîñòàòíüî âèñîêà ïëàñòè÷í³ñòü ñîðò³â ³ ñåëåêö³éíèõ ôîðì òðè-òèêàëå, ïîâ'ÿçàíà ç îáìåaeåíîþ ãåíåòè÷íîþ ð³çíîìàí³òí³ñòþ âèõ³äíîãî ìàòåð³àëó, ïîòðå-áóº ïîë³ïøåííÿ øëÿõîì çáàãà÷åííÿ ãåíî-ôîíäó ö³º¿ êóëüòóðè é ï³äâèùåííÿ åôåêòèâ-íîñò³ ¿¿ ñåëåêö³¿ ð³çíèìè ìåòîäàìè [3].Âàaeëèâå çíà÷åííÿ äëÿ ñåëåêö³éíîãî ïî-ë³ïøåííÿ òðèòèêàëå ìຠéîãî ñò³éê³ñòü äî Ìåòà. Ïðîàíàë³çóâàòè ð³âåíü ïåðåõðåñíî¿ ñò³éêîñò³ îòðèìàíèõ ñîëå-òà îñìîñò³éêèõ êë³òèííèõ ë³í³é ³ ðîñëèí-ðåãåíåðàíò³â òðèòèêàëå îçèìîãî äî îñìîòè÷íîãî òà ñîëüîâîãî ñòðåñ³â. Ìåòîäè. Êóëüòóðè òêàíèí ³ îðãàí³â in vitro, ñåëåêö³¿ in vitro, á³îõ³ì³÷í³, ñòàòèñòè÷íèé àíàë³ç. Ðåçóëüòàòè. Âñòàíîâëåíî, ùî ñòàá³ëüí³ñòü ïðîÿâó îçíàêè ïåðåõðåñíî¿ ñò³éêîñò³ ÿê äî ñîëüîâîãî, òàê ³ äî îñìîòè÷íîãî ñòðåñ³â ó îòðèìàíèõ êë³òèííèõ ë³í³é òðèòèêàëå îçèìîãî áóëà íà äîñèòü âèñîêîìó ð³âí³ -äî ê³íöÿ øîñòîãî ïàñàaeó âèaeèâàëî â³ä 50 äî 76% êàëþñ³â. Ïîêàçàíî, ùî, íåçâàaeàþ÷è íà íàÿâí³ñòü ó ñåëåêòèâíîìó ñåðåäîâèù³ ñóáëåòàëüíî¿ êîíöåíòðàö³¿ ñòðåñ-ôàêòîðà (ìàí³òó/õëîðèäó íàòð³þ), ñò³éê³ êë³òèíí³ ë³í³¿ òðèòèêàëå àêòèâíî ïðîäîâaeóâàëè ðîñòè é íàêîïè÷óâàòè á³îìàñó. Âèÿâëåíî, ùî ó ë³í³¿ '38/1296' íàéá³ëüø ñò³éêèìè äî îñìîòè÷íîãî òà ñîëüîâîãî ñòðåñ³â áóëè êë³òèíí³ ë³í³¿ 5Ë/ñë òà 5Ë/îñ â³äïîâ³äíî, à â ñîðòó 'Îáð³é' -1Ñ/ñë òà 1Ñ/îñ â³äïîâ³äíî, îñê³ëüêè âîíè ìàëè íàéâèùó ÷àñòêó aeèâèõ êàëþñ³â òà ïðèð³ñò á³îìàñè çà ñåëåêòèâíèõ óìîâ, à ðîñëèíè-ðå-ãåíåðàíòè ç íèõ -íàéâèùèé ð³âåíü âèaeèâàííÿ ï³ñëÿ 䳿 êîìïëåêñó àá³îòè÷íèõ ñòðåñîð³â. Ñîëåñò³éê³ êë³òèíí³ ë³í³¿ îáîõ ãåíîòèï³â òðèòèêàëå îçèìîãî ïîð³âíÿíî ç êîíòðîëåì õàðàêòåðèçóâàëèñü òàêîae äîñòîâ³ðíî âèùèì âì³ñòîì â³ëüíîãî ïðîë³íó çà 䳿 ñåëåêòèâíèõ ÷èííèê³â. Îäåðaeàí³ ðåçóëüòàòè ìîaeóòü ñâ³ä÷èòè ïðî òå, ùî êë³òèíí³ ë³í³¿ òà ðîñëèíè-ðåãå-íåðàíòè òðèòèêàëå ìàþòü ãåíåòè÷íî îáóìîâëåíó îçíàêó ñò³éêîñò³ äî ñòðåñîâèõ ôàêòîð³â. Âèñíîâêè. Ïåðåâ³ðêà îçíàê ñò³éêîñò³ äî àá³îòè÷íèõ ñòðåñîð³â çàñâ³ä÷èëà äîñèòü âèñîêèé ð³âåíü ïåðåõðåñíî¿ òîëåðàíòíîñò³ îòðèìàíèõ êë³òèííèõ ë³í³é îáîõ ãåíîòèï³â òðèòèêàëå ÿê äî ñîëüîâîãî, òàê ³ äî îñìîòè÷íîãî ñòðåñ³â. Ñò³éê³ñòü äî ñîëüîâîãî òà îñìîòè÷íîãî ñòðåñ³â âèä³ëåíèõ in vitro êë³òèí çáåðåãëàñü ó ³íäóêîâàíèõ ðîñëèíàõ ³ íà îðãàí³çìîâîìó ð³âí³ çàáåçïå÷èëà ï³äâèùåííÿ òîëåðàíòíîñò³ äî àá³îòè÷íèõ ôàêòîð³â ñåðåäîâèùà. Ïîêàçàíî, ùî çàâäÿêè çàãàëüíèì íåñïåöèô³÷íèì ìåõàí³çìàì ñò³éêîñò³ ðåçèñòåíòí³ñòü êàëþñíèõ êóëüòóð òðèòèêàëå äî îäíîãî àá³îòè÷íîãî ñòðåñîðà ìîaeå ïðèçâîäèòè äî ï³äâèùåííÿ òîëåðàíòíîñò³ é äî ³íøîãî.Êëþ÷îâ³ ñëîâà : òðèòèêàëå îçèìå, îñìîòè÷íèé ñòðåñ, ñîëüîâèé ñòðåñ, ñò³éê³ñòü, êë³òèíí³ ë³í³¿, ðîñëèíè-ðåãåíå-ðàíòè. àá³îòè÷íèõ ñòðåñîâèõ ÷èííèê³â äîâê³ëëÿ, çîêðåìà äî ïîñóõè òà çàñîëåííÿ ´ðóíò³â, ùî äàñòü çìîãó çá³ëüøèòè ïëîù³ ï³ä íèì ó ðàéîíàõ ç íåñïðèÿòëèâèìè êë³ìàòè÷íèìè óìîâàìè [4, 5]. Ïîñ...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.