Chemoattractants monocyte protein-1 (MCP-1) is a low molecular weight cytokine, secreted by many cells of the body. The synthesis of MCP-1 can induce the lipopolysaccharides of bacteria, a range of cytokines. It is believed that MCP-1 regulates the migration and infiltration of tissue by monocytes, T-lymphocytes memory, natural killer cells, is involved in differentiation of naive T-helper cells. There is evidence oncogenic and antitumor activity of MCP-1 at different stages of tumor progression. The aim of this work was the determination and comparison of serum level of MCP-1 by infection with Helicobacter (H.) pylori in precancerous conditions and gastric cancer.Material and methods.Surveyed with the informed consent 204 patients with precancerous diseases of the stomach, 40 patients with gastric cancer and 40 healthy volunteers. Patients at esophagogastroduodenoscopy conducted the fence of material for target biopsy for histological and microbiological studies. Blood on the immunoassay climbed in the morning on an empty stomach from the cubital vein in a volume of 5 ml in a vial without preserving agent, the serum was separated by centrifugation for 10 minutes. By ELISA in the serum of patients determined the level of MCP-1, titer of total antibodies to the cytotoxin-associated protein of H. pylori. The obtained data statistically processed.Results. The amount of MCP-1 in serum of the patients significantly exceeded the data of healthy individuals in all groups of comparison. Values of MCP-1 during exacerbation of gastric ulcer was higher than patients with exacerbation of chronic not atrophic and focal atrophic gastritis. The concentration of MCP-1 > 280 pg/ml was detected in 17.6% of patients with pre-cancerous conditions of the stomach. When ulceration process there was a strong direct correlation of the severity of its course and the number of MCP-1 in serum. Serum level of MCP-1 in 5.0% of patients with gastric cancer was at the upper limit of normal values, and 77.5% of patients exceeded it. Indicator in gastric cancer significantly prevailed over the values in precancerous conditions, was correlated with the tumor stage. The level of MCP-1 in serum of the examined healthy volunteers, patients with exacerbation of chronic gastritis, gastric ulcer disease, polyposis, gastric cancer was correlated with the titers of total antibodies to the cytotoxin-associated protein of H. pylori.Conclusion. Determination of serum level of MCP-1 in precancerous conditions and gastric cancer is diagnostically valuable. Comparison of indicator values with the titers of total antibodies to the cytotoxin- associated protein of H. pylori prognostically significant, as it allows to assess the intensity of the induction signal to production of a cytokine.
Резюме. Ульцерогенез слизистой оболочки гастродуоденальной зоны является сложным много этапным процессом, фазы его контролируются взаимодействием и каскадной активацией про и про тивовоспалительных цитокинов. В данном обзоре представлен анализ научных данных о влиянии цитокинов на ульцерозный и репаративный процессы, определен спектр их диагностических и тера певтических возможностей. Исследование цитокинового статуса больных язвенной болезнью желуд ка и двенадцатиперстной кишки и, в ряде случаев, генов цитокинов позволит прогнозировать течение заболевания, эффективность базисной и эрадикационной терапии, проводить коррекцию лечения.
Helicobacter pylori is a unique microorganism capable of long-term colonization of the gastric mucosa, induction of the inflammatory process, antigenic mimicry and immune evasia. Flagella proteins, adhesins, invasive and aggressive enzymes, cytotoxin-associated protein, vacuolating cytotoxin can have a damaging effect on stomach epithelial cells. Recognition of molecular patterns of Helicobacter pylori by stomach cell receptors initiates activation of adapter proteins, protein kinases and transcription factors, leading to the production of proinflammatory cytokines, infiltration by neutrophilic granulocytes, absorption and killing of microorganisms by phagocytes with presentation of antigens to lymphocytes, while the activity and completeness of phagocytosis remain at a low level. Activation of CD8+-, CD16+- lymphocytes is accompanied by cytotoxic effect on both Helicobacter pylori and epithelial cells of the gastric mucosa. Weak immunogenicity of Helicobacter pylori antigens limits the production of anti-Helicobacter antibodies. Thus, activation of immune factors, in most cases, does not lead to complete elimination of the pathogen, but can aggravate the pathomorphological changes of the gastric epithelium.
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