Background/Aim. The studies of hydroxyapatite (HAp) and growth factors as the materials used for direct pulp capping have produced conflicting results for both the issue of inflammmatory response and the issue of calcified bridge formation. Calcium hydroxyapatite/poly(lactide-co-glycolide) is a bioresorbable polymer with demonstrated good characteristics as the carrier for bone morphogenetic protein (BMP) necessary in bone tissue regeneration. The role of growth factors in dental tissue reparation (in both reactionary and reparative dentinogenesis) represents the new foundation and provides a different approach to dental pulp treatment. Growth factors-TGF-beta 1directly induce morphological and functional differentiation of neodontoblasts. The aim of this experimental study was to investigate the effect of calcium hydroxyapatite/poly(lactide-coglycolide) HAp/PLGA and growth factors (TGF-β1) in the formation of a calcified tissuedentine bridgeon the teeth of our experimental model. Methods. Rodent (rabbit) teeth were used as the animal model. After the trepanation of pulp space with sterile steel drills, the pulp was capped with calcium hydroxyapatite/poly(lactide-co-glycolide) HAp/PLGA (experimental group I; n=60); calcium hydroxyapatite/poly(lactide-co-glycolide) HAp/PLGA combined with TGF-β1 growth factor (experimental group II; n=60), and there was a control group of intact teeth (n=20). The experiment was performed in general anesthesia. The animals were kept alive for 1, 3 and 6 months. The extracted teeth were adequately prepared for scanning electron microscopy.Results. Scanning electron microscopy (SEM) demonstrated that the number of teeth with calcified tissue in the form of dental bridges in the HAp/PLGA+TGF-β1 group was statistically significantly greater 6 months (66.67%) than 3 months after the treatment (26.67%), at the statistical significance level of p<0.05. Conclusion. Direct pulp capping covers the artificially exposed dental pulp and makes possible the formation of a dentine bridge (a tubular structure composed of reparative dentine) in the period of 3 months.
Background / Aim. Hydroxyapatite (HAp) is one of the most commonly used calcium phosphate bioceramics with osteoconductive properties. Growth factors (ТGF-β) are capable of directly inducing morphological and functional differentiation of neodontoblasts. The aim of this paper was to investigate the effectiveness of biomaterials in combination with growth factors in the creation of new dentine and obturation of the root canal apex in the teeth of our experimental model. Methods. Rodent (rabbit) teeth were used as the experimental animal model. After pulp removal with a pulp extirpator in vital pulpectomy, the biomaterial was applied using a Lentulo spiral in the apex portion at the level of the physiological foramen apicale. The experiment was performed in general anesthesia. Animals were kept alive for 3, 6 and 12 months. The extraxted teeth were prepared for scanning electron microscopy (SEM) observation. Results. Using SEM, it was found that the number of teeth with newly created dentine and apex canal obturation was greater 12 months after the treatment. Conclusion. In our experimental groups, apex obturation of the dental root canal with newly created dentine took place.
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