Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The objective of this research was to identify QTL and single nucleotide polymorphism (SNP) markers associated with ASB resistance in recombinant inbred lines (RIL) derived from a cross between BAT 477 and NY6020-4 common bean. One hundred and twenty-six F6:7 RIL were phenotyped for ASB in the greenhouse. Disease severity was scored on a scale of 1–9. Genotyping was performed using whole genome resequencing with 2x common bean genome size coverage, and over six million SNPs were obtained. After being filtered, 72,017 SNPs distributed on 11 chromosomes were used to conduct the genome-wide association study (GWAS) and QTL mapping. A novel QTL region of ~4.28 Mbp from 35,546,329 bp to 39,826,434 bp on chromosome Pv03 was identified for ASB resistance. The two SNPs, Chr03_39824257 and Chr03_39824268 located at 39,824,257 bp and 39,824,268 bp on Pv03, respectively, were identified as the strongest markers associated with ASB resistance. The gene Phvul.003G175900 (drought sensitive, WD repeat-containing protein 76) located at 39,822,021 – 39,824,655 bp on Pv03 was recognized as one candidate for ASB resistance in the RIL, and the gene contained the two SNP markers. QTL and SNP markers may be used to select plants and lines for ASB resistance through marker-assisted selection (MAS) in common bean breeding.
Common bean (Phaseolus vulgaris L.) breeding lines (BL) UPR‐Mp‐34 (Reg no. CV‐313, PI 701379) and UPR‐Mp‐42 (Reg no. CV‐314, PI 701380) were developed at the University of Puerto Rico. UPR‐Mp‐34 was derived from A 195/‘PC 50’//SEA 5, and UPR‐Mp‐42 was developed from BAT 477/NY6020‐4//PRA154 common bean populations. Both BL have intermediate levels of resistance (mean scores, 4–6) to two inoculations of the PRI21 Macrophomina phaseolina (Tassi) Goidanich isolate (the causal agent of ashy stem blight) compared with BAT 477, SEA 5, TARS‐MST1, and XAN 176, which mostly had susceptible scores (7–9) in the greenhouse and field evaluations. UPR‐Mp‐34 has an indeterminate growth habit Type III, has a cream seed (average weight, 26.5 g per 100 seeds), initiates flowering at 32 d after planting, and reaches harvesting maturity at 86 d. Mean seed yield was 730 and 340 kg ha−1 in Isabela and Lajas, respectively. UPR‐Mp‐42 possesses black seed (23.8 g per 100 seeds), has a determinate growth habit Type I, initiates flowering at 30 d, and matures at 76 d. Mean seed yield was 750 kg ha−1 in both locations. The intermediate levels of ashy stem blight resistance from both BL could be combined with other sources of resistance and introgressed into common bean cultivars from different market classes.
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