Limitation of 3D construction ability, complex preparation process, and developing customer demands have promoted efforts to find low-cost, rapid prototyping, and operation simple methods to produce novel functional devices in the near future. Amongst various techniques, 3D-printed technology is a promising candidate for the fabrication of biosensors and biomedical detection devices with a wide variety of potential applications. This review offers four important 3D printing techniques towards biosensors and biomedical detection devices and its applications. The principle and printing process of 3D-printed technologies will be generalized and the printing performance of many 3D printer will be compared. Despite the limitation of 3D-printed resolution, these 3D-printed technologies have already shown promising applications in many biosensors and biomedical detection devices such as 3D-printed microfluidic devices, 3D-printed optical devices, 3D-printed electrochemical devices and 3D-printed integrated devices. Some of the most representative examples will also be discussed here, demonstrating that the 3D-printed technology can rationally design biosensor and biomedical detection devices and achieve important applications in microfluidic, optical, electrochemical and integrated devices.

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