Radioresistance is an important cause of cancer treatment failure. Circular RNAs (circRNAs) play crucial roles in cancer development, including the radioresistance. This research aimed to determine the function and related mechanism of circ_0086720 in the radioresistance of non-small cell lung cancer (NSCLC). The expression of circ_0086720, miR-375, and Spindlin 1 (SPIN1) was measured using a quantitative real-time polymerase chain reaction (qRT-PCR). Cell survival fraction was analyzed using colony formation assay, and cell apoptosis was monitored b y flow cytometry assay. The activities of caspase3 and caspase9 were assessed using the corresponding commercial kits. The ed by western blot. Bioinformatics analysis was performed to predict the targets of circ_0086720 and miR-375. Dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, and RNA pull-down assay were conducted to validate the interaction between miR-375 and circ_0086720 or SPIN1. The animal model was constructed to ascertain the role of circ_0086720 in vivo. The expression of circ_0086720 and SPIN1 was increased in the radioresistant NSCLC tissues, while miR-375 expression was decreased. The circ_0086720 knockdown sensitized NSCLC cells to the radiation to further inhibit cell survival and induce cell apoptosis. Circ_0086720 targeted miR-375 and suppressed miR-375 expression, and miR-375 bound to SPIN1 to impair SPIN1 expression. miR-375 deficiency or SPIN1 overexpression could attenuate circ_0086720 knockdown-mediated radiosensitivity. The circ_0086720 knockdown also enhanced radiosensitivity to further block tumor growth in vivo. To conclude, circ_0086720 downregulation enhanced the sensitivity of NSCLC to radiation by regulating the miR-375/SPIN1 axis, contributing to the improvement of the radiotherapies in NSCLC.
This work aimed to explore the application value of intelligent region of interest (ROI) detection algorithm in evaluating renal dynamic imaging information data of renal function in patients with chronic heart failure (CHF). 52 patients with CHF were selected as the research objects and grouped according to the level of renal function into an experimental group (14 cases) and an observation group (38 cases). In addition, 10 healthy people were selected as the research objects, as the control group. Different calculation methods of glomerular filtration rate (GFR) were used to evaluate the renal function of patients, and the ROI detection algorithm-assisted delineation was compared with traditional manual delineation. It was found that the GFR of the experimental group calculated by different methods was 52.58 mL/min/1.73m2 and 43.77 mL/min/1.73m2, respectively, which was significantly lower than that of the observation group ( P < 0.05 ), and that in both groups was lower than that of the control group ( P < 0.05 ). In addition, the unilateral renal function values of the two auxiliary delineations were both 13.61 mL/min/1.73 m2, while the results of the two manual delineations were significantly different ( P < 0.05 ). Therefore, renal dynamic imaging based on the ROI detection algorithm to evaluate the renal function of CHF patients showed a great improvement in effectiveness, sensitivity, stability, and efficiency compared with the traditional manual delineation, which was worthy of clinical promotion.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.