Fungal phytotoxic secondary metabolites are poisonous substances to plants produced by fungi through naturally occurring biochemical reactions. These metabolites exhibit a high level of diversity in their properties, such as structures, phytotoxic activities, and modes of toxicity. They are mainly isolated from phytopathogenic fungal species in the genera of Alternaria, Botrytis, Colletotrichum, Fusarium, Helminthosporium, and Phoma. Phytotoxins are either host specific or non-host specific phytotoxins. Up to now, at least 545 fungal phytotoxic secondary metabolites, including 207 polyketides, 46 phenols and phenolic acids, 135 terpenoids, 146 nitrogen-containing metabolites, and 11 others, have been reported. Among them, aromatic polyketides and sesquiterpenoids are the main phytotoxic compounds. This review summarizes their chemical structures, sources, and phytotoxic activities. We also discuss their phytotoxic mechanisms and structure–activity relationships to lay the foundation for the future development and application of these promising metabolites as herbicides.

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Soil wind erosion is a global problem that leads to increasingly serious regional land degradation, where the need for windbreak and sand fixation services (WSFS) is substantial. Inner Mongolia plays an important role in global semiarid and arid areas and the severe land degradation resulting from soil wind erosion warrants an urgent solution. However, the mechanism of influence of various driving factors on windbreak and sand fixation services is still not fully studied. In this paper, the revised wind erosion equation (RWEQ) model was used to synthesize the monthly spatiotemporal dynamics of soil wind erosion modulus (SWEM) and WSFS in Inner Mongolia from January 2000 to February 2020 from a semi-monthly scale. The influencing factors of WSFS were examined from both natural and anthropogenic aspects. Results show that over the past 20 years, the average SWEM in Inner Mongolia was 118.06 t ha−1 yr−1, the areas with severe wind erosion were mainly concentrated in the desert areas in the southwest of Inner Mongolia, and the forests in the northeast suffered less soil wind erosion. Meanwhile, the average WSFS was 181.11 × 108 t yr−1, with the high-value areas mainly located in major deserts, sandy land, and the area bordering Mongolia in the north and the low-value areas mainly located in the densely forested northeast and the Gobi Desert in the northwest. Both SWEM and WSFS showed a clear downward trend and a certain periodicity over the past 20 years. WSFS showed two peaks a year (April and October). Among the natural factors, precipitation and NDVI showed a significant correlation with WSFS and were identified as the main driving factors of WSFS, whereas temperature had no significant correlation. Among the anthropogenic factors, farming and animal husbandry intensity and GDP showed a positive correlation with WSFS, whereas population showed a negative correlation. These four types of factors were identified as socio-economic factors that drive WSFS. Meanwhile, WSFS did not show any significant correlation with the administrative area. Land use change contributed to a large proportion of WSFS change, thereby suggesting that the intensity of human activities is another central driver of WSFS.

Ustilaginoidins are a class of bis-naphtho-γ-pyrone mycotoxins produced by the pathogen Villosiclava virens of rice false smut, which has recently become one of the most devastating diseases in rice-growing regions worldwide. In this research, the nanobody phage display library was established after an alpaca was immunized with the hemiustilaginoidin F-hapten coupled with bovine serum albumin (BSA). Heterologous antigen selection and combing trypsin with competition alternant elution methods were performed for nanobody screening. Two nanobodies, namely, Nb-B15 and Nb–C21, were selected for the establishment of indirect competitive enzyme-linked immunosorbent assays (ic-ELISAs). For Nb–B15 and Nb-C21, their IC50 values were 11.86 μg/mL and 11.22 μg/mL, and the detection ranges were at 3.41–19.98 μg/mL and 1.17–32.13 μg/mL, respectively. Two nanobodies had a broad spectrum to quantify the contents of total ustilaginoidins in rice samples according to cross-reactivity. The recognition mechanisms of Nb-B15 and Nb-C21 against ustilaginoidin A were elucidated by molecular modeling and docking. The key amino acid sites for the binding of Nb–B15 or Nb–C21 to ustilaginoidin A were mainly located in the FR1 and CDR1 regions. As Nb-B15 was superior to Nb–C21 in the aspects of protein expression, ELISA titer, and tolerance to organic solvents, it was selected for application in the detection of actual contaminated rice samples. The total ustilaginoidin contents of rice samples were analyzed by Nb–B15-based ic–ELISA and HPLC-DAD, between which the results were found to be consistent. The developed immunoassay based on the nanobody from the alpaca can be employed as a rapid and effective method for detection of total utilaginoidins in contaminated rice samples.

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