Marine microalgae can be used as sustainable protein sources in many fields with positive effects on human and animal health. DAPTMGY is a heptapeptide isolated from Isochrysis zhanjiangensis which is a microalga. In this study, we evaluated its anti-photoaging properties and mechanism of action in human immortalized keratinocytes cells (HaCaT). The results showed that DAPTMGY scavenged reactive oxygen species (ROS) and increase the level of endogenous antioxidants. In addition, through the exploration of its mechanism, it was determined that DAPIMGY exerted anti-photoaging effects. Specifically, the heptapeptide inhibits UVB-induced apoptosis through down-regulation of p53, caspase-8, caspase-3 and Bax and up-regulation of Bcl-2. Thus, DAPTMGY, isolated from I. zhanjiangensis, exhibits protective effects against UVB-induced damage.
Microalgae
are important biological sources of marine active peptides
and renewable biological resources. Isochrysis zhanjiangensis has been widely used in biological ultrafiltration membranes and
aquaculture. However, there are relatively few studies on its component
structure and diverse activities. In this study, the mechanism of
action of previously isolated pentapeptides (AYP, Ala-Tyr-Ala-Pro-Glu)
on inflammation and tumor angiogenesis was evaluated. The results
showed that AYP could effectively inhibit the invasion and migration
of human umbilical vein endothelial cells (HUVECs) and HT1080 cells
by downregulating the expression of MMP-2/-9, independent of cytotoxicity.
Especially after 100 μM AYP treatment, the ability to inhibit
migration was about 67.7% ± 1.9 for HT1080 cells and 63.6% ±
1.3 for HUVECs, respectively. In addition, the activity of iNOS and
COX-2 was decreased by inhibiting the oversecretion of VEGF in HT1080
cells induced by CoCl2 and the activation of VEGFR-2 in
HUVECs and by regulating PI3K/AKT and Ras/MAPK signaling pathways.
It can prevent inflammation and block tumor angiogenesis. Therefore,
AYP is expected to become a drug or functional food to prevent and
treat tumor angiogenesis.
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