Kentucky bluegrass (KB, Poa pratensis) is one of the most widely used cool-season turfgrass species, but it is sensitive to drought stress. Molecular studies in KB are hindered by its large and complex genome structure. In this study, a comparative transcriptomic study was conducted between a short and long period of water deficiency. Three transcriptome libraries were constructed and then sequenced by using leaf RNA samples of plants at 0, 2, and 16 h after PEG6000 treatment. A total of 199,083 differentially expressed genes (DEGs) were found. The Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed that DEGs were enriched in “Plant hormone signal transduction” and “MAPK signaling pathway-Plant”. Some key up-regulated genes, including PYL, JAZ, and BSK, were involved in hormone signaling transduction of abscisic acid, jasmonic acid, and brassinosteroid and possibly these genes play important roles in coping with drought stress in KB. Furthermore, our results showed that the concentrations of ABA, JA and BR increased significantly with the extension of the drought period. The specific DEGs encoding functional proteins, kinase and transcription factors, could be valuable information for genetic manipulation to promote drought tolerance of KB in the future.

Myomectomy during CS was a safe and an effective procedure.

Since its first application toward displacement measurements in the early-1960s, laser feedback interferometry has become a fast-developing precision measurement modality with many kinds of lasers. By employing the frequency-shifted optical feedback, microchip laser feedback interferometry has been widely researched due to its advantages of high sensitivity, simple structure, and easy alignment. More recently, the laser confocal feedback tomography has been proposed, which combines the high sensitivity of laser frequency-shifted feedback effect and the axial positioning ability of confocal microscopy. In this paper, the principles of a laser frequency-shifted optical feedback interferometer and laser confocal feedback tomography are briefly introduced. Then we describe their applications in various kinds of metrology regarding displacement measurement, vibration measurement, physical quantities measurement, imaging, profilometry, microstructure measurement, and so on. Finally, the existing challenges and promising future directions are discussed.

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