This study aimed to diagnose Sarcina ventriculi in lambs with haemorrhagic abomasitis using histopathological and real-time PCR methods. The material used in this study consisted of 43 abomasum tissues recovered from lambs presenting bleeding, ulcer, gas or a combination of these in the abomasum, that were brought for necropsy to the Department of Pathology of the Veterinary Faculty, Selcuk University. The recovered samples were stored in a 10% formaldehyde solution for histopathological examinations and in Eppendorf tubes at −20 °C for PCR examinations. All the samples were analyzed by histopathological and PCR methods. While S. ventriculi pyruvate decarboxylase (PDC) amplicon was determined by real-time PCR in 17 cases, the packaged form of Sarcina-like bacteria was found microscopically in 3 cases only. In this study, the diagnosis of S. ventriculi in cases of lambs presenting bleeding, ulcers and gas in the abomasum or haemorrhagic abomasitis simultaneously at the necropsy was performed using histopathological and real-time PCR methods; in parallel, the real-time PCR method for the diagnosis of S. ventriculi-derived haemorrhagic abomasitis in lambs was also optimized.
Urothelial carcinoma are malignant tumours originating from the epithelial layer of the urinary bladder. In this instance, a case of infiltrative type urothelial carcinoma in the urinary bladder of a 2-year-old terrier dog was defined histopathologically and immunohistochemically. The material of the presented case consisted of urinary bladder tissue samples that were surgically extirpated from a two-year-old terrier-breed female dog that applied to the Department of Surgery of the Faculty of Veterinary Medicine, Selcuk University with the complaint of hematuria. The tumour brought to the pathology laboratory was 11*10*12 cm in size and had finger-shaped extensions. Its outer surface was rough and hemorrhagic. Tissues were fixed in 10% buffered formalin and paraffin blocks were obtained by going through the necessary routine follow-up procedures. Afterwards, sections were taken and subjected to Hematoxylin-Eosin, Masson's Trichrome and immunohistochemical staining. As a result of the pathological and immunohistochemical examinations of the tumoral tissue samples taken from the urinary bladder of a two-year-old terrier dog, the diagnosis of infiltrative type urothelial carcinoma was reached, and the case was discussed with the information provided by the literature. In addition, immunohistochemically, intense PCNA and VEGF staining has been associated with malignancy.
Helicobacter species such as H. heilmannii, H. pylori, H. felis, H. bizzozeronii and H. salomonis have been identified in cats and dogs, and research suggesting that these species may be zoonotic agents and has been studied intensified in recent years. The aim of this study was to reveal the presence, comparing the histopathological findings and Real-time PCR results of H. felis, H. heilmannii, and H. pylori in the stomach and liver tissues taken during the necropsies of owned, stray or shelter dogs. The material of the study consisted of stomach and liver tissues taken from 35 dogs that died for different reasons and were brought for necropsy. DNA copies of H. heilmannii were detected by Real-time PCR in the liver samples of 30 dogs using H. heilmannii-specific primers. In the case of gastric samples, Real-time PCR detected H. heilmannii in 13 cases, H. pylori in 3 cases, both H. heilmannii and H. pylori in 13 cases, and H. felis, H. heilmannii and H. pylori in 3 cases. Microscopically, neutrophil leukocyte infiltration, epithelial degeneration, fibrosis and oedema in the lamina propia, and lymphoplasmacytic cell infiltration were determined in the stomachs. In the Hemotoxylin Eosin staining of the sections, 5 cases and 14 cases in the Warthin–Starry staining were found positive for Helicobacter-like microorganisms. Microscopically, dissociation of the remark cords and hydropic degeneration in hepatocytes, and focal mononuclear cell infiltrations in some sections were determined in the livers. In conclusion, with this study, it was understood that Real-time PCR analyzes are very useful in the diagnosis of H. felis, H. heilmannii, and H. pylori. However, histopathological examinations are necessary to associate the presence of bacteria with the development of the disease.
Bakır zehirlenmesi, bakırın vücuda alınımı ve atılımı arasındaki dengesizlikten kaynaklanan patolojik bir durumdur. Akut zehirlenme genellikle antihelmintik ilaçlar, mineral karışımları veya aşırı miktarda çözünür bakır tuzlarının uygulanmasından sonra görülür. Sunulan bu çalışmanın materyalini 900 toklu oluşturmaktadır. Hasta hayvanlarda abdominal sancı, ishal, hemoglobinüri, ikterus ve ölüm bulunmaktaydı. Yapılan nekropsiler sonucu deri altı yağ dokusu, serozalar ve organların etrafındaki yağ dokuda şiddetli sarılık belirlendi. Karaciğerin koyu kahve ya da sarımtırak renkte ve kolay parçalandığı görüldü. Safra keseleri dolgun, safra koyu renkte ve granüler yapıdaydı. Böbreklerin oldukça şişkin ve koyu siyah renkte olduğu görüldü. İdrar keseleri hemoglobinüriye bağlı olarak koyu kırmızı renkte idrar içermekteydi. Histopatolojik muayenede hepatositlerde hidropik dejenerasyon, vakuoler dejenerasyon, yağlanma, multifokal nekrozlar ve dissosiasyon, hepatositlerin sitoplazmasında sarı kahve renkte granüllerin yanı sıra hepatosit sitoplazmaları ve safra kanalcıklarında sarımsı-yeşil renkte safra pigmentlerine rastlandı. Portal alanlarda mononükleer hücre infiltrasyonları ile safra kanalı artışı belirlendi. Karaciğer kesitlerine Rodaninle yapılan boyamalarda hepatositlerin sitoplazmalarında kırmızımsı renkte pozitif boyanmalar tespit edildi, Böbreklerde tubul epitellerinde şişkinlik ve vakuoler dejenerasyon, bilurubin pigmenti dikkati çekti. Sunulan bu çalışmada koyunlarda görülen ve yem katkısından kaynaklandığı belirlenen akut bakır zehirlenmesinin klinik, toksikolojik ve patolojik bulgularının ortaya konulması ve son yıllarda oldukça sık karşılaşılan yem katkısı kaynaklı zehirlenme olaylarına dikkat çekilmesi amaçlanmıştır.
Infectious bursal disease (IBD) is a viral disease that causes significant economic losses in young chickens, characterized by lymphoid depletion and inflammation in the bursa Fabricius (BF). The incidence of the disease shows an increasing trend all over the world. Active and passive immunization is very important as well as strict hygiene measures in combating outbreaks. However, the fact that live-attenuated vaccines (mild, intermediate, hot) used for this purpose cause immunosuppression because of bursal damage is seen as an important limitation. In this study, it was aimed to histopathologically investigate the effects of commercial IBD vaccines originating from WF2512 (intermediate plus/hot, orally with drinking water) on BF under routine broiler rearing conditions. For this, BFs of 55 Ross 308 hybrid breed chickens (50 test, 5 controls) from five different broiler farms were used. In addition to standard vaccines, the IBD vaccine was given on day 15, and five samples from each farm were obtained 10 days later (25th day). After the first sampling, the second BF sampling was performed at the age of 38 days. Histopathological bursal lesion score was applied to evaluate the effectiveness of the vaccine. Accordingly, it was determined that the bursal lesion score, which increased slightly to moderately in the first samples, decreased in the second samples (27-61%). This was accepted as an indication that the bursal damage, which increased with IBD vaccine administration, diminishes over time and that histological regeneration was increased.
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