Stripe rust resistance transferred from Thinopyrum intermedium into common wheat was controlled by a single dominant gene, which mapped to chromosome 1B near Yr26 and was designated YrL693. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a highly destructive disease of wheat (Triticum aestivum). Stripe rust resistance was transferred from Thinopyrum intermedium to common wheat, and the resulting introgression line (L693) exhibited all-stage resistance to the widely virulent and predominant Chinese pathotypes CYR32 and CYR33 and to the new virulent pathotype V26. There was no cytological evidence that L693 had alien chromosomal segments from Th. intermedium. Genetic analysis of stripe rust resistance was performed by crossing L693 with the susceptible line L661. F(1), F(2), and F(2:3) populations from reciprocal crosses showed that resistance was controlled by a single dominant gene. A total 479 F(2:3) lines and 781 pairs of genomic simple sequence repeat (SSR) primers were employed to determine the chromosomal location of the resistance gene. The gene was linked to six publicly available and three recently developed wheat genomic SSR markers. The linked markers were localized to wheat chromosome 1B using Chinese Spring nulli-tetrasomic lines, and the resistance gene was localized to chromosome 1B based on SSR and wheat genomic information. A high-density genetic map was also produced. The pedigree, molecular marker data, and resistance response indicated that the stripe rust resistance gene in L693 is a novel gene, which was temporarily designated YrL693. The SSR markers that co-segregate with this gene (Xbarc187-1B, Xbarc187-1B-1, Xgwm18-1B, and Xgwm11-1B) have potential application in marker-assisted breeding of wheat, and YrL693 will be useful for broadening the genetic basis of stripe rust resistance in wheat.
Four red spring wheat (Triticum aestivum L.) lines, L658 (Reg. No. GP-971, PI 672537), L693 (Reg. No. GP-972, PI 672538), L696 (Reg. No. GP-973, PI 672539), and L699 (Reg. No. GP-974, PI 672540), were developed at the Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University of China from the cross 'MY11'/YU25, the latter of which was derived from a cross between common wheat (Triticum aestivum, 2n = 6x = 42, AABBDD) and Thinopyrum intermedium (Host) Barkworth & D.R. Dewey (2n = 6x = 42; JJJ s J s SS). The four wheat lines have high levels of resistance to Fusarium head blight (FHB) (mainly caused by Fusarium graminearum Schwabe) similar to those of the FHB-resistant genotypes 'Sumai 3' and 'Wangshuibai' based on results of experiments conducted in three diferent years in three diferent areas. In addition to FHB resistance, these lines also have strong resistance to both stripe rust (caused by Puccinia striiformis Westend. f. sp. tritici), with infection type (IT) = 0 or 0;, and powdery mildew (caused by Erysiphe graminis f. sp. tritici), with IT = 0. The results obtained in the Henan Province of China also show that these lines have suitable agronomic and morphological traits. These results indicate that the novel resistant wheat lines L658, L693, L696, and L699 could play an important role in wheat genetic improvement, especially for FHB, stripe rust, and powdery mildew resistance.
Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a destructive disease in wheat. A population consisting of 229 F and F plants derived from the cross PI 672538 × L661 was used to evaluate the reactions to FHB. The FHB resistance data distribution in the F population indicates that some quantitative trait loci (QTLs) were controlling the FHB resistance in PI 672538. We further detected two major QTLs (Qfhs-2B, Qfhs-3B) from analysis of the resistance data and the PCR-amplified results using WinQTLCart 2.5 software. Qfhs-2B, flanked by Xbarc55-2B and Xbarc1155-2B, explained more than 11.6% of the phenotypic variation of the percentage of diseased spikelets (PDS), and Qfhs-3B, flanked by Xwmc54-3B and Xgwm566-3B, explained more than 10% of the PDS phenotypic variation in the F population. In addition, Qfhs-3B was different from Fhb1 in terms of the pedigree, inheritance, resistance response, chromosomal location, and marker diagnosis. We also detected QTLs for other disease resistance indices, including the percentage of damaged kernels and 1,000-grain weight, in similar chromosomal regions. Therefore, the FHB resistance of PI 672538 was mainly controlled by two major QTLs, mapped on 2B (FhbL693a) and 3B (FhbL693b). PI 672538 could be a useful germplasm for improving wheat FHB resistance.
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