A B S T R A C TInfluenza viruses with multiple subtypes have highly virulent in humans, of which influenza hemagglutinin (HA) is the major viral surface antigen. Simultaneous and automated detection of multiple influenza HA are of great importance for early-stage diagnosis and operator protection. Herein, a magnetism and size mediated microfluidic platform was developed for point-of-care detection of multiple influenza HA. With multiplex microvalves and computer program control, the detection process showed high automation which had a great potential for avoiding the high-risk virus exposure to the operator. Taking advantage of magnetism and size mediated multiple physical fields, multiple influenza HA could be simultaneous separation and detection depended on different-size magnetic beads. Using high-luminance quantum dots as reporter, this assay achieved high sensitivity with a detection limit of 3.4 ng/mL for H7N9 HA and 4.5 ng/mL for H9N2 HA, and showed excellent specificity, anti-interference ability and good reproducibility. These results indicate that this method may propose new avenues for early detection of multiple influenza subtypes.
This paper is based on the investigation and interview methods to probe on multimedia English teaching from the students' angle of view and cognitive psychology. The author achieves results after conducting a preliminary comparison into the relationships between traditional English teaching and multimedia English teaching by investigation and interview.
In this study, a vocabulary cognition experiment is designed with cross-language startup paradigm from the cognitive perspective to analyze the time course of English vocabulary semantic accessibility of college students, the influence of words and speech information on English semantic accessibility, and the relationship between English learning and Chinese learning and their mutual influence on the cognitive process. The experiment uses event-related potential technology to record the sensory and cognitive processes of the brain online in a continuous and real time manner. The components of the scalp area are separated to determine the cortical areas activated by the mental activity, thus determining the internal nerve source of the explicit behavior. This study discusses English vocabulary learning from the perspective of cognitive linguistics, mainly to understand learners' cognitive characteristics and cognitive process of English vocabulary learning. In view of this phenomenon, it is proposed that learners should establish the cognitive schema of English vocabulary to form the semantic network of English vocabulary, overcome the negative transfer of mother tongue, and achieve vocabulary cognition through a great deal of English input and mastery of context knowledge. This study complements the research on vocabulary cognition of college students, and the results provide theoretical guidance for college students' English learning and language teaching.
Different from traditional Internet communication manner, the Blog has its own advantages and features. It can increase communication between teachers and students, expand the field of study, the blog used in the teaching of English writing helps improve students' English writing ability, students should be encouraged to use the blog wisely to improve their English writing.
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