In this manuscript, an immense sensitive single-core double-slotted square-shaped SPR biosensor externally coated with gold nano films is designed and critically analyzed. The sensor shows a wide range of sensing capacity between 1.41 to 1.49 RIU. Two circular slots are constructed to hold more analytes inside the slots. The sensor shows extremely sensitive behavior with the highest sensitivity of 36000nm/RIU with the maximum amplitude sensitivity of 1380 RIU−1. The maximum resolution of the sensor is 9×10−5 RIU. The sensor parameters are optimized in COMSOL Multiphysics software by observing fabrication tolerance using percentage variations of parameters. The experimental data of the proposed sensor also shows good polynomial fitting and a high figure of merit (FOM). Because of the newer structure, high sensitivity, large detection range, high stability and high resolution the proposed sensor can be a tough candidate in the realm of bio-sensing and biochemical applications.
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