Summary During a nematode biodiversity survey from 2012 to 2014 in Shenzhen, China, ten nematode populations (SZX1301–SZX1310) of Xiphinema were recovered from rhizosphere of different plants, namely Acacia mangium (SZX1306), A. confuse (SZX1309), Blechnum orientale (SZX1301, SZX1302, SZX1307, SZX1308), Litchi chinensis (SZX1304, SZX1310) in Tianxinshan and Gleichenia linearis (SZX1303, SZX1305) in Yangmeikeng environmental monitoring sites. Morphological and molecular profiles of these populations were determined. Three species of Xiphinema, i.e., X. hunaniense Wang & Wu, 1992, X. brasiliense Lordello, 1951 and X. americanum Cobb, 1913 sensu lato were identified using morphological characters and molecular data of partial 18S and 28S D2–D3 rDNA expansion segments. Four populations (SZX1301–SZX1304) were X. hunaniense, one population (SZX1305) X. brasiliense, and five populations (SZX1306–SZX1310) X. americanum s.l.. Phylogenetic analysis based on sequences of the 28S rDNA D2–D3 expansion segment revealed these three species are all distinct species and supported a close relationship with their corresponding species. This is the first report of X. hunaniense, X. brasiliense and X. americanum s.l. in their hosts except for L. chinensis.
323 SummaryStudies were conducted to characterize morphological and molecular profiles of two isolates of Paratrichodorus porosus (SZ1 and SZ2) which were recovered from Acacia mangium in Tianxinshan and Gleichenia linearis in Yangmeikeng environmental monitoring sites in Shenzhen, China, respectively. Analysis of morphometric, morphological and molecular characters revealed these two Shenzhen isolates are identical to P. porosus. Measurements of both study isolates lie within the ranges for P. porosus. It is typologically characterized by possessing a clearly swollen body cuticle after fixation, an onchiostyle ventrally curved, 46 -58 μm long, a pharyngeal bulb usually with a well developed anterior-dorsal intestinal overlap, a secretoryexcretory pore opening between the nerve ring and anterior end of pharyngeal bulb, 90 -110 μm from the anterior end, a reproductive system with didelphic, amphidelphic, without spermathecae, a pore-like vulva in ventral view and occupying 52.0 % -59.5 % of total body length from anterior end, a short and barrel-shaped vagina with small sclerotizations, a pair of ventromedian advulvar body pores located prevulvar and postvulvar, a rounded tail and a subterminal anus in females. The sequence analysis based on partial rDNA 18S gene and 28S D2/D3 expansion segment confirm its identity as P. porosus. This is the first report of P. porosus associated with A. mangium and G. linearis.
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