We investigate continuous observation of dynamic phenomena through quantitative phase microscopy. We conduct imaging studies using optically computed phase microscopy, a novel imaging technology developed in our lab, to the best of our knowledge. Inevitably, continuous phase imaging is affected by phase wrapping artifacts, which affects correct quantification of sample dynamics. To address this issue, we develop a 3D unwrapping method that exploits data correlation in space as well as in time. We validate our 3D phase unwrapping method using simulated data. We further validate 3D phase unwrapping using experimental data and demonstrate quantitative phase imaging that accurately characterizes sample dynamics. We image the nanoscale motion of the sample actuated by a piezo transducer (PZT). We calculate the displacement using 3D unwrapped phase, and the result is consistent with the known motion of the PZT. We also image live cells that were detaching from the substrate of the petri dish. The optical path length calculated using 3D unwrapped phase increases as the dry mass of the cell becomes more concentrated during the detachment process.
The capability to image subtle mechanical motion at cellular and sub-cellular scales can be used to study how extracellular particles interact with cultured cells and, more generally, how cells interact with their environment. However, current technologies need to provide sufficient spatial resolution, temporal resolution, and motion sensitivity to image cellular and sub-cellular motion in the en face plane. To address this unmet need, we investigate a full-field Doppler phase microscopy (FF-DPM) technology based on an innovative optical computation strategy that enables depth-resolved imaging and phase quantification. In this study, we validated the motion tracking (displacements and velocities) capability of FF-DPM by imaging samples actuated by a piezo transducer (PZT). We demonstrated FF-DPM imaging of magnetic particles under different conditions with different motion characteristics. Our results show that free particles (suspended in a cell culture medium) had a significantly larger magnitude of motion than particles adhered to a cell. The key innovation of this study is the use of an optical computation strategy to perform depth-resolved phase quantification and Doppler measurement. The FF-DPM will have a significant impact, as it provides a unique capability to quantitatively measure subtle motion for models based on cultured cells.
Background and aims: To investigate the usefulness of IL-6、TNF-α、Protein C(PC) and Thromboelastography (TEG) to serve as a predictor of portal vein thrombosis (PVT) in patients with liver cirrhosis. At the same time, we explored the clinical significance of the above indicators in the disease progression. Methods: A total of 123 patients with liver cirrhosis were recruited from May 2021 to December 2021, according to the imaging findings. They were divided into the PVT group (n=52) and the non-PVT group (n=71). Furthermore, patients with PVT were divided into plasma transfusion groups (n=13) and non-plasma transfusion groups (n=39). The basic general information, past medical history, laboratory, and imaging examination data were collected and analyzed. Results: In univariate analysis, there was no significant difference in IL-6, PC, R, Angle, MA, and CI between the two groups (P > 0.05). TNF-α in the PVT group was significantly lower than that in the non-PVT group (P =0.001). K in the PVT group was significantly higher than that in the non-PVT group (P =0.031) There was no significant difference in IL-6, TNF-α, PC, or TEG between different Child-Pugh classification groups (P>0.05). There was no significant difference in TEG between the plasma transfusion group and the non-plasma transfusion group. In Binary logistic regression analysis, TNF-α (OR=0.9881,95%CI=0.971,0.990,P<0.001) ,K(OR=1.28,95%=1.053,1.569,P=0.014) ,APTT (OR=0.753,95%CI=0.656,0.865,P<0.001) , diameter of portal vein (OR=1.310,95%CI=1.108,1.549,P=0.002)and the history of splenectomy or embolism (OR=7.565,95%CI=1.514,37.799,P=0.014)were related to the formation of PVT. Conclusions: TNF-α, K, APTT, the diameter of the portal vein and the history of splenectomy or embolism were the related factors of PVT formation, but IL-6 was not related to the formation of PVT.
In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial resolution in phase imaging. We evaluated the performance of complex-OCPM imaging using resolution targets and live cells. Our results show that the complex-OCPM system achieves quantitative phase imaging with sub-cellular resolution on label-free cells.
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